Engineered Nanomaterials: Linking Physical and Chemical Properties to Biology

工程纳米材料：将物理和化学特性与生物学联系起来

• 批准号: 8462273
• 负责人: KENT Ed PINKERTON
• 金额: $ 48.29万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-27 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8462273
• 关键词: Acute; Address; Affect; Asthma; Biological; Biology; Breathing; Caliber; Cell-Mediated Cytolysis; Cells; Charge; Chemistry; Deposition; Engineering; Exposure to; Goals; Histopathology; Hypersensitivity; Image; Inflammation; Inflammatory; Ingestion; Inhalation Toxicology; Investigation; Length; Link; Lung; Lung Inflammation; Measures; Methodology; Modeling; Modification; Monitor; Organ; Organism; Oxidative Stress; Pattern; Positioning Attribute; Principal Investigator; Property; Rattus; Resolution; Respiratory System; Respiratory tract structure; Risk Assessment; Series; Silicon Dioxide; Site; Surface; Techniques; Testing; Toxic effect; Wing; absorption; airway hyperresponsiveness; airway remodeling; arm; burden of illness; cell type; chemical property; cytotoxicity; density; in vitro Model; in vitro testing; in vivo; in vivo Model; insight; macrophage; nanomaterials; nanowire; novel; particle; physical property; programs; research study; response; single walled carbon nanotube; surface coating; uptake

The goal of this program is to systematically explore the influence of physicochemical properties of size, composition, surface lability as well as charge, density, and activity of engineered nanomaterials (ENMs). The goal of this project is to define the effect of these physical/chemical properties on how ENMs interact with the intact organism, including specific target organs and specific cell types within the target organs. We will focus one ofthe major classes of ENMs, the high aspect ratio nanomaterials (HARNMs), including single wall carbon nanotubes (SWNTs) and nanowires (NWs) of various lengths. The importance of size (diameter and length) and coatings (silica as one example) to affect toxicity, retention and translocation will be assessed. HARNM with differing physical/chemical property described above will be employed in a series of systematic examinations of absorption and distribution following inhalation/ingestion experiments. Primary and secondary target organ responses will be monitored, and the influence of HARNM exposure In a model of allergy will be assessed. The overall hypothesis Is that differences in composition, size, diameter and surface coating of HARNMs will modulate the in vivo uptake, distribution and biologic effects of HARNMs in a rat model. This hypothesis will be addressed in four specific aims that will determine the effect of HARNM on 1) deposition, retention and distribution to various organs; 2) respiratory system cytotoxicity, inflammation and airway remodeling; 3) oxidative stress in the respiratory system; and 4) exacerbation of ainway hyperresposiveness in a sensitive model. We will address these aims using a transdisciplinary approach that combines inhalation toxicology, chemistry, histopathology, high resolution imaging and novel methodologies developed at UC Davis that uniquely position us to successfully address the biological effects of these materials. The long term goal is to identify features of these materials that reduce their toxicity and biological effects. RELEVANCE (See instnjctions); To develop and evaluate techniques and approaches to assess the potential disease burden associated with exposures to ENMs. The goal of Project 2 is to define exposure effects of high aspect ratio nanomaterials (single walled carbon nanotubes and nanowires) in an in vivo inhalation model that includes airways hyperresonsiveness.

这个项目的目标是系统地探索尺寸的物理化学性质的影响，