Characterization and genetic analysis of aging in Daphnia
水蚤衰老的特征和遗传分析
基本信息
- 批准号:8529425
- 负责人:
- 金额:$ 26.04万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-09-01 至 2015-05-31
- 项目状态:已结题
- 来源:
- 关键词:AgeAgingAging-Related ProcessAnimal ModelBiological ModelsBiology of AgingCellsCollectionComplexCoupledDaphniaDependencyDevelopmentDominant-Negative MutationEcologyElementsEnvironmentFacultyFoodFresh WaterGene ExpressionGene Expression ProfileGenesGeneticGenetic VariationGenomeGenomicsGoalsIndividualInterventionInvestigationKnowledgeLaboratoriesLifeLife Cycle StagesLinkLongevityMethodsMicroarray AnalysisModelingMolecularOligonucleotidesOrganismParthenogenesisPathway interactionsPatternPerformancePhysiologicalPhysiological ProcessesPopulationProcessQuantitative GeneticsRegulationReproductionResearch PersonnelResourcesSystemSystems BiologyTSC1 geneTechniquesTestingTransgenic OrganismsVariantViralWorkage effectage relatedasexualdietary restrictiongenetic analysisgenetic variantgenome-wideinsightlife historymutantnovelnutritionoverexpressionpressurepromoterpublic health relevanceresponsetool
项目摘要
DESCRIPTION (provided by applicant): This project will develop the microcrustacean Daphnia as a model system for the biology of aging. Daphnia are freshwater microcrustaceans that reproduce through cyclic parthenogenesis, allowing laboratory investigators to generate thousands of genetically identical individuals easily. Biodemographic and physiological methods for quantifying genetic variation of lifespan and rates of aging will be coupled with analyses of genome-wide gene expression and the development of transgenic individuals. The goal is to establish a model system where information on aging can be integrated from molecular, cellular, physiological and demographic approaches and placed in the context of known ecological pressures exerted on aging and on the response to available resources. The proposed work includes A) characterization of aging in TCO, the Daphnia pulex clone whose entire genome has been sequenced, B) characterization of aging in a genetically diverse collection of new isolates from the field, C) characterization of the response to dietary restriction in the TCO clone and new isolates, D) application of whole-genome microarray technology to quantify global patterns of age-dependency in gene expression in TCO and new isolates, E) investigation of the scale and pattern of changes in age-dependent gene expression induced by dietary restriction, F) identification of novel genes and pathways with age- dependent regulation or whose expression is predictive of lifespan, G) development of a technique for heritable transformation of live Daphnia, H) overexpression of key genes involved in pathways known to regulate the effects of dietary restriction on aging, and I) observation of aging and response to dietary restriction in Daphnia clones where key genes have been overexpressed.
PUBLIC HEALTH RELEVANCE: Aging is the progressive decline of bodily faculties as individuals grow older. The development of new model organisms to understand the biology of aging allows for a broader understanding of the genetic mechanisms and consequences of potential interventions, such as dietary restriction, that reduce the negative effects of aging. In this work, a new model organism, Daphnia, is developed through the identification of genetic variants with different rates of aging, identification of genes with age-dependent expression, and determination of the effects of key genes on aging processes.
描述(由申请人提供):该项目将开发微型甲壳动物水蚤作为衰老生物学的模型系统。水蚤是淡水微型甲壳类动物,通过周期性孤雌生殖繁殖,使实验室研究人员能够轻松产生数千个基因相同的个体。用于量化寿命遗传变异和衰老速度的生物人口学和生理学方法将与全基因组基因表达和转基因个体发育的分析相结合。目标是建立一个模型系统,可以从分子、细胞、生理和人口学方法整合有关衰老的信息,并将其置于已知的对衰老施加的生态压力和对可用资源的响应的背景下。拟议的工作包括A)表征TCO(其整个基因组已测序的水蚤克隆)的衰老特征,B)表征来自该领域的遗传多样性新分离株的衰老特征,C)表征TCO克隆和新分离株对饮食限制的反应,D)应用全基因组微阵列技术来量化TCO和新分离株中基因表达的年龄依赖性的全局模式, E) 研究饮食限制引起的年龄依赖性基因表达变化的规模和模式,F) 鉴定具有年龄依赖性调节或其表达可预测寿命的新基因和途径,G) 开发活体水蚤可遗传转化的技术,H) 已知调节饮食限制对衰老影响的途径中涉及的关键基因的过度表达,I) 观察衰老和对饮食限制的反应 在关键基因被过度表达的水蚤克隆中。
公共卫生相关性:衰老是指随着个体年龄的增长,身体机能逐渐衰退。开发新的模型生物体来了解衰老的生物学,可以更广泛地了解遗传机制和潜在干预措施的后果,例如饮食限制,以减少衰老的负面影响。在这项工作中,通过鉴定具有不同衰老速率的遗传变异、鉴定具有年龄依赖性表达的基因以及确定关键基因对衰老过程的影响,开发了一种新的模式生物——水蚤。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jeffry L. Dudycha其他文献
Maternal effects in the model system Daphnia: the ecological past meets the epigenetic future
模型系统水蚤中的母体效应:生态过去与表观遗传未来相遇
- DOI:
10.1038/s41437-024-00742-w - 发表时间:
2025-01-08 - 期刊:
- 影响因子:3.900
- 作者:
Trenton C. Agrelius;Jeffry L. Dudycha - 通讯作者:
Jeffry L. Dudycha
Jeffry L. Dudycha的其他文献
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{{ truncateString('Jeffry L. Dudycha', 18)}}的其他基金
Characterization and genetic analysis of aging in Daphnia
水蚤衰老的特征和遗传分析
- 批准号:
8318132 - 财政年份:2010
- 资助金额:
$ 26.04万 - 项目类别:
Characterization and genetic analysis of aging in Daphnia
水蚤衰老的特征和遗传分析
- 批准号:
8728718 - 财政年份:2010
- 资助金额:
$ 26.04万 - 项目类别:
Characterization and genetic analysis of aging in Daphnia
水蚤衰老的特征和遗传分析
- 批准号:
8133386 - 财政年份:2010
- 资助金额:
$ 26.04万 - 项目类别:
Characterization and genetic analysis of aging in Daphnia
水蚤衰老的特征和遗传分析
- 批准号:
7983053 - 财政年份:2010
- 资助金额:
$ 26.04万 - 项目类别:
MUTATION, PLEIOTROPY AND THE GENETIC STRUCTURE OF AGING
突变、多效性和衰老的遗传结构
- 批准号:
6447538 - 财政年份:2001
- 资助金额:
$ 26.04万 - 项目类别:
MUTATION, PLEIOTROPY AND THE GENETIC STRUCTURE OF AGING
突变、多效性和衰老的遗传结构
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6636712 - 财政年份:2001
- 资助金额:
$ 26.04万 - 项目类别:
MUTATION, PLEIOTROPY AND THE GENETIC STRUCTURE OF AGING
突变、多效性和衰老的遗传结构
- 批准号:
6520594 - 财政年份:2001
- 资助金额:
$ 26.04万 - 项目类别:
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