Accelerator Mass Spectrometry Core

项目总结(见说明)： 核心B将负责通过加速器质谱仪(AMS)对从女性和男性心脏收集的DNA样本中随年龄变化的大气14C进行分析。心肌细胞、内皮细胞和成纤维细胞将分别进行评估，以确定它们的平均年龄和周转率。此外，~(14)C标记或~3H标记的胸腺嘧啶核苷在不同心肌细胞群中的水平 在生命过程中注射这种同位素的小鼠和狗将是在这个核心进行的分析的一部分。同位素的传递不仅可以在心肌细胞、内皮细胞和成纤维细胞中测量14C或~3H，还可以在包括血管平滑肌细胞(SMCs)和心脏干细胞(CSCs)在内的较小细胞类别中测量14C或~3H。通过这种方法，AMS将提供独立的年龄和更新价值 在动物和人类中，每种心脏细胞类别随着年龄和性别的变化而变化。

PROJECT SUMMARY (See instructions): Core B will be responsible for the analysis of atmospheric 14C by Accelerator Mass Spectrometry (AMS) in samples of DNA collected from female and male human hearts as a function of age. Cardiomyocytes, endothelial cells (ECs), and fibroblasts will be evaluated separately to define their average age and turnover rate. Additionally, the levels of 14C-labeled or 3H-labeled thymidine in the various cardiac cell populations of mice and dogs injected with this isotope during the course of life will be part of the assays to be performed in this core. The delivery of the isotope will allow the measurement of 14C or 3H not only in cardiomyocytes, ECs, and fibroblasts, but also in smaller cell classes including vascular smooth muscle cells (SMCs) and cardiac stem cells (CSCs). By this approach, AMS will provide an independent value of the age and renewal of each cardiac cell category with aging and gender in animals and humans.