Accelerator Mass Spectrometry Core

加速器质谱核心

• 批准号: 8464853
• 负责人: Bruce A Buchholz
• 金额: $ 25.03万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8464853
• 关键词: 2-cyclopentyl-5-(5-isoquinolylsulfonyl)-6-nitro-1H-benzo(D)imidazole; Age; Aging; Animal Model; Animals; Biological Assay; Blood Vessels; Canis familiaris; Cardiac; Cardiac Myocytes; Categories; Cells; DNA; Endothelial Cells; Female; Fibroblasts; Gender; Heart; Human; Individual; Instruction; Isotopes; Label; Life; Life Cycle Stages; Liquid substance; Mass Spectrum Analysis; Measurement; Mus; Physiological; Population; Radioactive; Radioisotopes; Sampling; Scintillation Counting; Smooth Muscle Myocytes; Stem cells; Thymidine; Tracer; accelerator mass spectrometry; human male; improved; instrument; stable isotope

PROJECT SUMMARY (See instructions): Core B will be responsible for the analysis of atmospheric 14C by Accelerator Mass Spectrometry (AMS) in samples of DNA collected from female and male human hearts as a function of age. Cardiomyocytes, endothelial cells (ECs), and fibroblasts will be evaluated separately to define their average age and turnover rate. Additionally, the levels of 14C-labeled or 3H-labeled thymidine in the various cardiac cell populations of mice and dogs injected with this isotope during the course of life will be part of the assays to be performed in this core. The delivery of the isotope will allow the measurement of 14C or 3H not only in cardiomyocytes, ECs, and fibroblasts, but also in smaller cell classes including vascular smooth muscle cells (SMCs) and cardiac stem cells (CSCs). By this approach, AMS will provide an independent value of the age and renewal of each cardiac cell category with aging and gender in animals and humans.

项目摘要（请参阅说明）： 核心B将通过加速器质谱法（AMS）对大气14C的分析，该样品在女性和男性人心脏收集的DNA样品中随着年龄的影响。心肌细胞，内皮细胞（EC）和成纤维细胞将分别评估以定义其平均年龄和周转率。此外，在各种心脏细胞种群中的14C标签或3H标记的胸苷的水平 在生命过程中注入这种同位素的小鼠和狗将成为该核心要进行的测定的一部分。同位素的递送将允许在心肌细胞，ECS和成纤维细胞中测量14C或3H，还可以在包括血管平滑肌细胞（SMC）和心脏干细胞（CSC）在内的较小细胞类别中进行测量。通过这种方法，AMS将提供年龄和更新的独立价值 每个心脏细胞类别的衰老和性别，动物和人类。