Co-registered photoacoustic and ultrasound imaging for non-invasive ovarian can

联合配准光声和超声成像在非侵袭性卵巢癌检测中的应用 和人物塑造 摘要 我们建议开发一种新的经阴道成像设备，使用共同注册的光声和 超声技术在卵巢癌双模式无创检测中的应用 和原位异常。光声技术提供了肿瘤血管生成的光学对比 以及肿瘤缺氧和超声(US)分辨率，适用于深达3-4厘米的病变。这个 非侵袭性成像卵巢早期血管生成变化和肿瘤缺氧的能力 成像方式将极大地加强对卵巢癌风险女性的护理。 从我们对正常、异常和恶性卵巢组织的体外成像中获得的数据 显示当恶性肿瘤和癌症时有显著不同的血管生成。 将异常卵巢组织与正常卵巢组织进行比较。我们已经共同开发了一个原型-- 注册的光声和美国系统，这使我们能够验证这一新的假设 影像设备将改善目前临床实践对早期无创性诊断的认识 卵巢癌和卵巢异常，将指导高危卵巢癌的外科治疗 病人。 在这项建议中，我们将1)开发一种经阴道光声/超声探头和系统，适用于 活体双模式非侵入性卵巢检测和特征；2)特征正常， 体外异常和癌变卵巢组织的成像结果与组织病理学和病理对照 微血管密度计数和分布以及平均光吸收；3)评估 预防性卵巢切除术患者活体成像的原型探头；4) 监测一组高危绝经前妇女的月经周期，以确定 可能与血管生成变化不同的功能和形态参数的周期性变化 与恶性过程有关。大约60名患者将被招募进行体外试验 在目标2中进行的研究，这将使我们能够验证拟议的 技术。大约40名患者将被招募参加AIM 3的体内研究，这将使我们能够 对该技术进行评估并验证体外实验结果。大约20%到25%的绝经前高- 风险患者将被招募参加AIM 4的体内研究，这将使我们能够评估 这项新技术在绝经前人群中。 该项目的成功完成将为改善目前的临床 实践：它将提供必要的技术，以提高确定是否存在癌症的能力 并避免对相当数量的女性进行手术。

Co-registered photoacoustic and ultrasound imaging for non-invasive ovarian cancer detection and characterization Abstract We propose to develop a new transvaginal imaging device using co-registered photoacoustic and ultrasound technique for dual-modality non-invasive detection and characterization of ovarian cancer and abnormality in situ. The photoacoustic technique provides optical contrast of tumor angiogenesis and tumor hypoxia with ultrasound (US) resolution for deeply seated lesions of up to 3-4 cm. The ability to image early angiogenesis changes as well as tumor hypoxia in the ovary with this non-invasive imaging modality would greatly enhance the care for women at risk for ovarian cancer. Data obtained from our ex vivo imaging of normal, abnormal and malignant ovarian tissue have demonstrated that there is a significantly different angiogenic formation when malignant cancers and abnormal ovarian tissues are compared to normal ovarian tissues. We have developed a prototype co- registered photoacoustic and US system, which allows us to validate the hypothesis that this new imaging device will improve the current clinical practice for non-invasive diagnosis of early stage ovarian cancers and ovarian abnormality and will guide surgical intervention of high-risk ovarian cancer patients. In this proposal, we will 1) develop a transvaginal photoacoustic/US probe and system suitable for in vivo dual-modality non-invasive detection and characterization of the ovary; 2) characterize normal, abnormal and cancerous ovarian tissue ex vivo by comparing imaging results with histopathology and microvessel density counts and distributions as well as average optical absorption; 3) evaluate the prototype probe for in vivo imaging of patients who are scheduled for prophylactic oophorectomy; 4) monitor a group of high-risk premenopausal women during their menstrual cycles to determine the cyclic changes in functional and morphological parameters that may differ from angiogenesis changes associated with the malignant process. Approximately 60 patients will be recruited for the ex vivo study in Aim 2, which will allow us to validate the initial sensitivity and specificity of the proposed technique. Approximately 40 patients will be recruited for the in vivo study in Aim 3, which will allow us to evaluate the technique and validate the ex vivo results. Approximately 20 to 25 premenopausal high- risk patients will be recruited for the in vivo study in Aim 4, which will allow us to assess the efficacy of this new technique within a premenopausal population. The successful completion of the project will provide a means to improve the current clinical practice: it will provide necessary technology to improve the ability to determine if a cancer is present and to avoid surgery in a substantial number of women.