Tissue and Pathology Core

人类和小鼠胃肠道组织样本的采集、病理表征和分子分析是该SPORE应用程序所有项目的基础和组成部分。Dana-Farber /哈佛癌症中心（DF/HCC）SPORE胃肠道（GI）癌症组织和病理学核心将继续为SPORE研究人员提供基本和高级病理学服务，以支持每个研究项目以及职业发展和发展项目中概述的研究。组织和病理学中心将通过与GI肿瘤库和DF/HCC病理学标本库的互动收集、储存和提供组织标本，进行组织学分析并提供人食管、结肠、胰腺和GI间质瘤（GIST）的解释性病理学分析。核心将确保使用适当分类的GI癌症组织样本，并提供给所有拟定的免疫组织化学和分子分析。此外，核心致力于使用先进的分子病理学工具表征胃肠道肿瘤。将提供对小鼠和人类胃肠道肿瘤的成功分子分析至关重要的各种服务，包括：实验研究中使用的所有肿瘤样本的组织病理学审查和质量控制分析;组织微阵列的构建，以改善和简化所有免疫组织化学分析;冷冻和福尔马林固定的选择和宏观切片，石蜡包埋（FFPE）组织样本，以确保在实验研究中使用适当的细胞区室;用于免疫组织化学（IHC）应用的抗体优化;在小鼠和人体组织中进行广泛的IHC染色的性能和分析，直接支持项目;从所有组织类型（新鲜冷冻和存档FFPE）中分离核酸提取物，用于分子分析。最后，核心将评估和实施新技术，如胃肠道肿瘤切片的离体孵育及其随后的分子表征，以响应治疗，生物标志物的多重化和复杂的细胞成像技术。

The acquisition, pathologic characterization and molecular analysis of human and mouse GI tissue samples is a fundamental and integral for all projects of this SPORE application. The Dana-Farber / Harvard Cancer Center (DF/HCC) SPORE in Gastrointestinal (GI) Cancer Tissue and Pathology Core will continue to provide basic and advance pathology services to SPORE investigators in support the studies outlined in each of the Research Projects, as well as in Career Development and Developmental Projects. The Tissue and Pathology Core will collect, store and provide tissue specimens, through interactions with GI Tumor banks and the DF/HCC Pathology Specimen Locator, perform histologic analyses and provide interpretive pathologic analysis of human esophageal, colonic, pancreatic and GI stromal tumors (GISTs). The Core will ensure that appropriately classified GI cancer tissue samples are used and supplied for all immunohistochemical and molecular analyses proposed. In addition, the Core is committed to the characterization of gastrointestinal neoplasms using advanced molecular pathology tools. A variety of services critical to successful molecular analysis of mouse and human GI tumors will be provided, including; histopathologic review and quality control analysis of all tumor samples utilized in experimental studies; construction of tissue micorarrays to improve and streamline all immunohistochemical analyses; selection and macrodlssection of frozen and formalin-fixed, paraffin-embedded (FFPE) tissue samples to ensure that appropriate cellular compartments are utilized in experimental studies; antibody optimizations for applications in immunohistochemistry (IHC); performance and analysis of a broad range of IHC stains in mouse and human tissues in direct support of the projects; and isolation of nuclei acid extractions from all tissue types (fresh frozen and archival FFPE) for molecular analyses. Finally, the Core will evaluate and implement novel technologies such as ex vivo incubation of GI tumor sections and their subsequent molecular characterization in response to treatment, multiplexing of biomarkers and sophisticated cellular imaging techniques.