Tissue and Pathology Core

人和小鼠胃肠道组织的获取、病理学特征及分子生物学分析 samples是此SPORE应用程序所有项目的基础和组成部分。达纳法伯/ 哈佛癌症中心（DF/HCC）胃肠道（GI）癌症组织和病理学核心的孢子将 继续为SPORE研究人员提供基础和高级病理学服务，以支持研究 在每个研究项目中概述，以及在职业发展和发展 项目组织和病理学中心将通过以下方式收集、储存和提供组织标本： 与GI肿瘤库和DF/HCC病理学标本库的相互作用，进行组织学检查 分析并提供人类食管、结肠、胰腺和胃肠道的解释性病理分析 间质肿瘤（GIST）。核心将确保适当分类的GI癌组织样品是 用于并提供所有免疫组织化学和分子分析。此外，核心是 致力于使用先进的分子病理学表征胃肠道肿瘤 工具.将提供对小鼠和人GI肿瘤的成功分子分析至关重要的各种服务。 提供，包括;组织病理学审查和质量控制分析中使用的所有肿瘤样本 实验研究;组织微阵列的构建，以改善和简化所有 免疫组织化学分析;冷冻和福尔马林固定，石蜡包埋的 （FFPE）组织样本，以确保在 实验研究;免疫组织化学（IHC）应用的抗体优化;性能 分析小鼠和人体组织中广泛的IHC染色，直接支持项目; 从所有组织类型（新鲜冷冻和存档FFPE）中分离核酸提取物， 分析。最后，核心将评估和实施新的技术，如体外孵育， GI肿瘤切片及其随后响应于治疗的分子表征，多重 生物标志物和复杂的细胞成像技术。

The acquisition, pathologic characterization and molecular analysis of human and mouse Gl tissue samples is a fundamental and integral for all projects of this SPORE application. The Dana-Farber / Harvard Cancer Center (DF/HCC) SPORE in Gastrointestinal (Gl) Cancer Tissue and Pathology Core will continue to provide basic and advance pathology services to SPORE investigators in support the studies outlined in each of the Research Projects, as well as in Career Development and Developmental Projects. The Tissue and Pathology Core will collect, store and provide tissue specimens, through interactions with Gl Tumor banks and the DF/HCC Pathology Specimen Locator, perform histologic analyses and provide interpretive pathologic analysis of human esophageal, colonic, pancreatic and Gl stromal tumors (GISTs). The Core will ensure that appropriately classified Gl cancer tissue samples are used and supplied for all immunohistochemical and molecular analyses proposed. In addition, the Core is committed to the characterization of gastrointestinal neoplasms using advanced molecular pathology tools. A variety of services critical to successful molecular analysis of mouse and human Gl tumors will be provided, including; histopathologic review and quality control analysis of all tumor samples utilized in experimental studies; construction of tissue micorarrays to improve and streamline all immunohistochemical analyses; selection and macrodissection of frozen and formalin-fixed, paraffin-embedded (FFPE) tissue samples to ensure that appropriate cellular compartments are utilized in experimental studies; antibody optimizations for applications in immunohistochemistry (IHC); performance and analysis of a broad range of IHC stains in mouse and human tissues in direct support of the projects; and isolation of nuclei acid extractions from all tissue types (fresh frozen and archival FFPE) for molecular analyses. Finally, the Core will evaluate and implement novel technologies such as ex vivo incubation of Gl tumor sections and their subsequent molecular characterization in response to treatment, multiplexing of biomarkers and sophisticated cellular imaging techniques.