Tissue and Pathology Core

人和小鼠Gl组织的获取、病理特征及分子分析 样品是这个孢子应用的所有项目的基础和组成部分。The Dana-Farber/ 哈佛癌症中心(DF/HCC)胃肠道(Gl)癌组织和病理学核心孢子 继续为孢子研究人员提供基本和高级病理学服务，以支持研究 在每个研究项目以及职业发展和发展中概述 项目。组织和病理核心将通过以下方式收集、存储和提供组织标本 与G1肿瘤库和DF/HCC病理标本定位器相互作用，执行组织学 分析和提供人类食道、结肠、胰腺和胃的解释性病理分析 间质瘤(GIST)。该中心将确保适当分类的G1癌症组织样本 用于所有提出的免疫组织化学和分子分析。此外，核心是 致力于使用先进的分子病理学来表征胃肠道肿瘤 工具。对成功进行小鼠和人类G1肿瘤的分子分析至关重要的各种服务将是 提供，包括：所有使用的肿瘤标本的组织病理学审查和质量控制分析 实验研究；构建组织微阵列以改善和简化所有 免疫组织化学分析.冰冻石蜡包埋福尔马林固定标本的选择和大体解剖 (FFPE)组织样本，以确保适当的细胞隔间在 实验研究.免疫组织化学中应用的抗体优化(IHC).性能 以及对小鼠和人类组织中广泛的IHC染色进行分析，以直接支持该项目； 以及从所有类型的组织(新鲜的、冷冻的和存档的FFPE)中分离核酸提取液用于分子 分析。最后，核心将评估和实施新技术，如体外孵化 GL肿瘤切片及其后续分子特征对治疗、多路转移的反应 生物标志物和复杂的细胞成像技术。

The acquisition, pathologic characterization and molecular analysis of human and mouse Gl tissue samples is a fundamental and integral for all projects of this SPORE application. The Dana-Farber / Harvard Cancer Center (DF/HCC) SPORE in Gastrointestinal (Gl) Cancer Tissue and Pathology Core will continue to provide basic and advance pathology services to SPORE investigators in support the studies outlined in each of the Research Projects, as well as in Career Development and Developmental Projects. The Tissue and Pathology Core will collect, store and provide tissue specimens, through interactions with Gl Tumor banks and the DF/HCC Pathology Specimen Locator, perform histologic analyses and provide interpretive pathologic analysis of human esophageal, colonic, pancreatic and Gl stromal tumors (GISTs). The Core will ensure that appropriately classified Gl cancer tissue samples are used and supplied for all immunohistochemical and molecular analyses proposed. In addition, the Core is committed to the characterization of gastrointestinal neoplasms using advanced molecular pathology tools. A variety of services critical to successful molecular analysis of mouse and human Gl tumors will be provided, including; histopathologic review and quality control analysis of all tumor samples utilized in experimental studies; construction of tissue micorarrays to improve and streamline all immunohistochemical analyses; selection and macrodissection of frozen and formalin-fixed, paraffin-embedded (FFPE) tissue samples to ensure that appropriate cellular compartments are utilized in experimental studies; antibody optimizations for applications in immunohistochemistry (IHC); performance and analysis of a broad range of IHC stains in mouse and human tissues in direct support of the projects; and isolation of nuclei acid extractions from all tissue types (fresh frozen and archival FFPE) for molecular analyses. Finally, the Core will evaluate and implement novel technologies such as ex vivo incubation of Gl tumor sections and their subsequent molecular characterization in response to treatment, multiplexing of biomarkers and sophisticated cellular imaging techniques.