Tissue and Pathology Core

组织和病理学核心

• 批准号: 8933245
• 负责人: Massimo Loda
• 金额: $ 24.7万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-31 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8933245
• 关键词: Acids; Advanced Development; Anatomy; Antibodies; Area; Barrett Esophagus; Biological; Biological Assay; Biological Markers; Biopsy; Blood; Blood specimen; Cancer Center; Cathepsins; Cell Nucleus; Clinical Data; Collaborations; Collection; Colorectal; Colorectal Cancer; Commit; Consent; Correlation Studies; Databases; Development; Diagnosis; Disease model; Dysplasia; Dysplasia in Barrett' s Esophagus; Emerging Technologies; Ensure; Esophageal; Evaluation; Fluorescent in Situ Hybridization; Formalin; Freezing; Fresh Tissue; Future; Gastrointestinal Neoplasms; Gastrointestinal Stromal Tumors; Genetic Markers; Goals; Heterogeneity; Histocompatibility Testing; Histologic; Histology; Histopathology; Human; Image; Image Analysis; Imaging Techniques; Imaging technology; Imatinib; Immunohistochemistry; Institution; Institutional Review Boards; Investigation; KRAS2 gene; Link; MEK inhibition; Malignant neoplasm of gastrointestinal tract; Metastatic Neoplasm to the Liver; Methodology; Modeling; Molecular; Molecular Analysis; Mus; Nucleic Acids; Pancreas; Paraffin Embedding; Pathologic; Pathology; Patients; Performance; Process; Protocols documentation; Quality Control; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Sampling; Services; Signal Pathway; Signal Transduction; Specimen; Staining method; Stains; Techniques; Technology; Tissue Banking; Tissue Banks; Tissue Microarray; Tissue Sample; Tissues; Tumor Bank; Vitamin D3 Receptor; Work; Xenograft procedure; career development; cellular imaging; cohort; flexibility; gastrointestinal; human tissue; improved; innovation; insight; laser capture microdissection; molecular pathology; mouse model; mutant; neoplastic; new technology; novel diagnostics; novel strategies; novel therapeutics; pre-clinical; repository; research clinical testing; research study; response; screening; success; tissue preparation; tool; tumor

The acquisition, pathologic characterization and molecular analysis of human and mouse Gl tissue samples is a fundamental and integral for all projects of this SPORE application. The Dana-Farber / Harvard Cancer Center (DF/HCC) SPORE in Gastrointestinal (Gl) Cancer Tissue and Pathology Core will continue to provide basic and advance pathology services to SPORE investigators in support the studies outlined in each of the Research Projects, as well as in Career Development and Developmental Projects. The Tissue and Pathology Core will collect, store and provide tissue specimens, through interactions with Gl Tumor banks and the DF/HCC Pathology Specimen Locator, perform histologic analyses and provide interpretive pathologic analysis of human esophageal, colonic, pancreatic and Gl stromal tumors (GISTs). The Core will ensure that appropriately classified Gl cancer tissue samples are used and supplied for all immunohistochemical and molecular analyses proposed. In addition, the Core is committed to the characterization of gastrointestinal neoplasms using advanced molecular pathology tools. A variety of services critical to successful molecular analysis of mouse and human Gl tumors will be provided, including; histopathologic review and quality control analysis of all tumor samples utilized in experimental studies; construction of tissue micorarrays to improve and streamline all immunohistochemical analyses; selection and macrodissection of frozen and formalin-fixed, paraffin-embedded (FFPE) tissue samples to ensure that appropriate cellular compartments are utilized in experimental studies; antibody optimizations for applications in immunohistochemistry (IHC); performance and analysis of a broad range of IHC stains in mouse and human tissues in direct support of the projects; and isolation of nuclei acid extractions from all tissue types (fresh frozen and archival FFPE) for molecular analyses. Finally, the Core will evaluate and implement novel technologies such as ex vivo incubation of Gl tumor sections and their subsequent molecular characterization in response to treatment, multiplexing of biomarkers and sophisticated cellular imaging techniques.

人和小鼠Gl组织的获取、病理特征和分子分析