Development of an automated noninvasive system for sleep restriction in rodents

开发用于啮齿类动物睡眠限制的自动化无创系统

• 批准号: 8455597
• 负责人: JIDONG FANG
• 金额: $ 42.04万
• 依托单位: AFASCI, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-12 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8455597
• 关键词: Acute; Adoption; Animal Model; Animal Testing; Animals; Anxiety; Automation; Behavior; Behavioral; Books; Boxing; Chronic; Computer software; Computers; Data; Development; Effectiveness; Electrodes; Electroencephalography; Electrophysiology (science); Evaluation; Experimental Designs; Feedback; Floor; Hand; Health; Human; Implant; Intervention; Investigation; Laboratories; Laboratory Animals; Light; Manuals; Marketing; Measures; Mental Health; Methods; Monitor; Motion; Mus; Outcome; Performance; Phase; Physiological; Polysomnography; Production; Protocols documentation; Public Health; Rattus; Records; Research; Rodent; Running; Schedule; Signal Transduction; Sleep; Sleep Deprivation; Sleep Disorders; Sleep Fragmentations; Small Business Innovation Research Grant; Social Behavior; Social Interaction; Societies; Supervision; Symptoms; System; Systems Development; Technology; Testing; Text; Time; Validation; base; cognitive function; commercialization; cooking; cost; drug discovery; feeding; flexibility; improved; innovation; neurobehavioral; novel; object recognition; physical conditioning; preference; programs; prototype; public health relevance; sensor; social; software development; software systems; success; tool; user-friendly

DESCRIPTION (provided by applicant): Sleep deprivation (SD) occurs universally in modern societies and causes significant social and financial harm. Numerous human and animal studies indicate that various forms of SD are deleterious to mental and physical health. Therefore, SD has been recognized as an unmet challenge to public health. There is increased need to study the effects of different forms of SD on physiological functions in animal models. However, better methods are sorely needed. Existing SD systems have low throughput and capacity and are difficult to use and expensive. In our Phase I project, we have developed an automatic sleep monitoring/sleep deprivation system for rodents. Our system is based on floor sensors, the data from which yield automatic sleep scoring. We demonstrated that "hands-off" automatic SD using our method is technically feasible. We have also verified the advantages of our system over other competing systems, namely, that our method is less invasive, more selective, less time-consuming, and easier to use. Based on the success of our Phase I project, our Phase II project will be focused on the commercialization of our research product. The objective of the present project is to develop a unified, non-invasive, high throughput and low cost SD system for mice and rats. Our specific aims are the follows. Aim 1. Development of a full-featured SD system with multiple lines of hardware and software products Aim 1a. Development of Hardware. We will refine, ruggedize, and make more reliable our mouse SD system while simultaneously lowering the costs for mass production. Because rats are used frequently in sleep research we will develop a rat SD system based on the mouse SD system. Aim 1b. Development of software. We will develop software products with the following innovative features: 1) Establish bi-directional information exchange through USB cable to perform three common types of SD: acute SD, sleep fragmentation and chronic sleep restriction; 2) Create a user-friendly interface for flexible experimental design on the host PC; 3) Expand system capability to perform simultaneous studies in as many as 64 rats or mice. 4) Extend the software functionality to perform group statistical analysis including group means, standard errors and data lists in animals from multiple groups of animals, and 5) Extend the software products to both 32-bit and 64-bit Windows XP, Vista and Windows 7 platforms. Aim 2. Evaluation and validation of the SD system, and development of "standardized" SD protocols The validation of the system includes: 1) verification of sleep loss and rebound following SD using EEG evaluation and verification of the robustness and ease of use of the system, 2) investigation of the consequences of SD on animals, especially social recognition/preference tasks, and 3) development of an experimental "cook book" through performing different types of SD to "standardize" SD protocols in order to facilitate comparison results generated by different laboratories.

描述（由申请人提供）：睡眠剥夺（SD）在现代社会普遍存在，并造成重大的社会和经济危害。许多人类和动物研究表明，各种形式的SD对身心健康有害。因此，可持续发展被认为是对公共卫生的一个尚未解决的挑战。在动物模型中，研究不同形式的SD对生理功能的影响的需求越来越大。然而，我们迫切需要更好的方法。现有的SD系统的吞吐量和容量都很低，而且难以使用且价格昂贵。在我们的一期项目中，我们开发了一个啮齿类动物睡眠自动监测/睡眠剥夺系统。我们的系统是基于地板传感器的，它的数据产生自动睡眠评分。我们证明了使用我们的方法的“不干涉”自动SD在技术上是可行的。我们还验证了我们的系统相对于其他竞争系统的优势，即，我们的方法侵入性更小，选择性更强，耗时更短，并且更容易使用。基于我们第一期项目的成功，我们的第二期项目将专注于我们的研究产品的商业化。本项目的目标是开发一种统一、无创、高通量、低成本的小鼠和大鼠SD系统。我们的具体目标如下。目的1。开发一个功能齐全的SD系统，包括多种硬件和软件产品。硬件开发。我们将改进，加固，使我们的鼠标SD系统更可靠，同时降低大规模生产的成本。由于大鼠经常被用于睡眠研究，我们将在小鼠SD系统的基础上开发大鼠SD系统。目标1 b。软件开发。我们将开发具有以下创新功能的软件产品：1)通过USB线建立双向信息交换，实现三种常见的SD：急性SD、睡眠碎片化和慢性睡眠限制；2)在主机上创建用户友好的界面，实现灵活的实验设计；3)扩展系统能力，可在多达64只大鼠或小鼠中同时进行研究。4)扩展软件功能，以执行群体统计分析，包括群体均值，标准误差和数据列表，从多个动物群体的动物。5)扩展软件产品的32位和64位Windows XP， Vista和Windows 7平台。目标2。评估和验证SD系统，制定“标准化”SD协议。系统验证包括：1)通过脑电图评估验证SD后的睡眠损失和反弹，验证系统的稳健性和易用性；2)调查SD对动物的影响，特别是社会识别/偏好任务；3)通过执行不同类型的SD来“标准化”SD协议，以便于不同实验室产生的结果进行比较。