Evaluation of Salmonella Symptomatic and Asymptomatic Pets: Study for Vet-LRN Pro

有症状和无症状宠物沙门氏菌的评估：Vet-LRN Pro 的研究

• 批准号: 8484751
• 负责人: Jing Cui
• 金额: $ 6万
• 依托单位: OHIO STATE DEPARTMENT OF AGRICULTURE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-16 至 2014-09-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8484751
• 关键词: Evaluation; Salmonella; Symptomatic; Asymptomatic; Pets

FDA CVM VET-LRN PROGRAM (U18) GRANT PROPOSAL Abstract This grant proposal from the Ohio Department of Agriculture (ODA) is in response to a Funding Opportunity Announcement (FOA) from the Food and Drug Administration's (FDA) Center for Veterinary Medicine (CVM) Veterinary Laboratory Response Network Study for the Vet-LRN Program (U18). The purpose of this proposal from ODA's Animal Disease Diagnostic Laboratory (ADDL)'s Bacteriology Section is to study the prevalence of Salmonella in fecal samples from both symptomatic and asymptomatic pets submitted to veterinary clinics in order to determine the potential zoonotic risks of Salmonella infected pets on human health. This proposal will address four Specific Aims: 1. To determine the prevalence of Salmonella in fecal samples from both symptomatic and asymptomatic animals brought to veterinary clinics. 2. To determine antimicrobial resistance patterns for all Salmonella isolates. 3. To determine serotypes for all Salmonella isolates. 4. To determine PFGE patterns for all Salmonella isolates. A successful implementation of the project outlined in the proposal is predicted for many reasons including the extensive experience of the ADDL's professional and technical staff with the isolation and identification of Salmonella. The qualifications of the staff are also presented. As part of the proposal, a detailed view of the laboratory facilities is provided. This grant proposal also addresses the CVM request for sample analysis commitment as well as the laboratory's ability and willingness to complete the proposed research in a timely manner. Several additional documents including the internal Standard Operating Procedures (SOP), Proficiency Testing (PT) results, letters of collaboration, certificate of AAVLD accreditation, and a summary of past achievements are provided along with the project narrative to showcase the laboratory's ability to provide the capacity and capability to conduct the project. We are confident that we can meet the objectives of this project and look forward to the opportunity to work with the FDA and other labs in its initiation.

FDA CVM VET-LRN认证（U18）资助提案 摘要 这项来自俄亥俄州农业部（ODA）的拨款提案是对一项 美国食品药品监督管理局的资助机会公告（FOA） (FDA)兽医中心（CVM）兽医实验室响应网络 Vet-LRN计划（U18） ODA动物疾病诊断实验室的这一建议的目的是 （ADDL）的细菌学组是研究粪便中沙门氏菌的流行情况 从有症状和无症状的宠物中抽取样本，送交兽医诊所 为了确定沙门氏菌感染宠物对人类的潜在人畜共患病风险， 健康该提案将涉及四个具体目标： 1.为了确定沙门氏菌在粪便样本中的流行率， 有症状和无症状的动物被带到兽医诊所。 2.确定所有沙门氏菌分离株的耐药性模式。 3.确定所有沙门氏菌分离株的血清型。 4.确定所有沙门氏菌分离株的PFGE模式。 预计提案中概述的项目将成功实施， 原因包括ADDL的专业和技术的丰富经验， 工作人员进行沙门氏菌的分离和鉴定。工作人员的资格是 并提出作为建议书的一部分， 提供了本赠款提案还解决了CVM的样品分析请求 承诺以及实验室的能力和意愿，以完成拟议的 研究及时。包括内部文件在内的其他文件 标准操作规程（SOP）、能力验证（PT）结果、 合作，AAVLD认证证书，以及过去成就的总结 与项目叙述沿着提供，以展示实验室的能力， 提供开展项目的能力和条件。但有信心 满足本项目的目标，并期待着有机会与 FDA和其他实验室在其启动。