Evaluation of Salmonella in Symptomatic and Asymptomatic Pets

有症状和无症状宠物中沙门氏菌的评估

• 批准号: 8484749
• 负责人: Timothy S Frana
• 金额: $ 6万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-16 至 2014-09-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8484749
• 关键词: Evaluation; Salmonella; Symptomatic; Asymptomatic; Pets

Project Summary The objective of this study is to determine the prevalence and types of Salmonella in fecal samples from both symptomatic and asymptomatic animals brought to veterinary clinics. Salmonella can be present in many different environments, including water, soil, insects, feces, wild and farm raised animals, raw foods, surfaces and animal feeds. Salmonellosis causes gastrointestinal disease in humans and animals. Symptoms in people include nausea, vomiting, abdominal cramps, diarrhea, fever and headache. Animals may also show signs of gastrointestinal disease, although they may harbor Salmonella bacteria without showing signs of clinical disease. Since 1999, a number of human salmonellosis outbreaks have been correlated with ingestion of Salmonella-contaminated animal feeds in Canada and the United States. What remains generally unknown is the effect that Salmonella contaminated feeds has on the health of pets. How frequently do cases of Salmonellosis occur in pets, and how frequently do they develop a carrier state, where do they harbor the bacteria but fail to exhibit clinical signs of disease? In addition, the various serotypes of Salmonella involved in pet disease have not been adequately characterized. In order to gain further insight into the prevalence of Salmonella infected animals following feed contamination, the FDA Center for Veterinary Medicine (CVM) is seeking proposals that will provide information on the prevalence of Salmonella in fecal samples obtained from diarrheic and non-diarrheic pets seeking veterinary care.. Laboratories will participate in a multi-center study and CVM will coordinate methods among the participants. Sampling protocols and culture methods will be standardized throughout the participating laboratories. Patient fecal samples will be cultured for Salmonella from using well established and validated methods. Serotyping methods will also be standardized among the participants. These isolates will be submitted to CVM upon completion of the study. A minimum of 100 symptomatic and 100 asymptomatic cases will be examined during a 12 month period. Participating laboratories will collaborate with other cooperative agreement laboratories to develop a final standardized protocol for their microbiologic isolations. They will also be required to participate in one round of proficiency testing per year. Further they will be required to report findings quarterly in a standardized format agreed upon by the cooperative laboratories.

项目摘要 本研究的目的是确定沙门氏菌的流行和类型的粪便样本，从两个 有症状和无症状的动物被带到兽医诊所。沙门氏菌可以存在于许多 不同的环境，包括水，土壤，昆虫，粪便，野生和农场饲养的动物，生食，表面 和动物饲料。沙门氏菌病引起人类和动物的胃肠道疾病。患者的症状 包括恶心、呕吐、腹部绞痛、腹泻、发烧和头痛。动物也可能表现出 胃肠道疾病，尽管它们可能含有沙门氏菌，但没有临床症状 疾病自1999年以来，一些人类沙门氏菌病暴发与摄入 沙门氏菌污染的动物饲料在加拿大和美国。目前尚不清楚的是 沙门氏菌污染的饲料对宠物健康的影响。有多频繁 沙门氏菌病发生在宠物身上，它们多久会发展成携带者状态，它们在哪里窝藏沙门氏菌， 细菌，但没有表现出疾病的临床症状？此外，涉及沙门氏菌的各种血清型 宠物疾病还没有得到充分的表征。为了进一步了解 沙门氏菌感染的动物在饲料污染后，FDA兽医中心（CVM） 寻求建议，以提供有关沙门氏菌在粪便样本中的流行情况， 寻求兽医护理的哺乳动物和非哺乳动物宠物。实验室将参与多中心研究 CVM将协调参与者之间的方法。采样方案和培养方法将 在所有参与实验室中进行标准化。将对患者粪便样本进行沙门氏菌培养 使用成熟和有效的方法。血清分型方法也将标准化， 参与者这些分离株将在研究完成后提交给CVM。至少100 将在12个月内对100名有症状的人和100名无症状的人进行检查。参与 实验室将与其他合作协议实验室合作， 进行微生物分离他们还将被要求参加一轮熟练程度测试， 每年测试。此外，还将要求他们每季度以商定的标准格式报告调查结果 合作实验室。