Fluorescence Microscopy and Cell Imaging Shared Resource

作为NCI支持的核心资源，UNM荧光显微镜资源的总体目标是 提供：a)通过用户访问最先进的设施和技术提供支持服务，b)用户 培训和个性化支持或协作以解决与癌症相关的问题，c)创新 通过测试和开发新的探测器和成像技术，d)通过 资源之旅、海报展示、年度开放日、研讨会和网页。 UNM荧光显微镜和细胞成像共享资源提供完整的荧光 显微成像服务，包括离子的比率成像、去卷积、共焦、高光谱 活体和固定样品的常规光学显微成像。手续费与服务费一致 与中等范围的全国范围内的费率和新墨西哥州癌症中心成员受益于20%的共同支付。这个 资源中心位于癌症研究设施二楼的四个房间里。 仪器定期通过墙外(NCRR、NCI)和墙内(UNM SOM)的组合进行升级 和癌症中心)资助。目前的乐器包括直立和倒置的蔡司、尼康和奥林巴斯 配备蔡司LSM 510、蔡司Meta和BioRad Radiance共聚焦扫描头和 TillVision比率测量系统。CRI Nuance、Andor Ixon、Orca和Zeiss数码相机提供低光 宽视场仪器的灵敏度和光谱图像采集。多达八种彩色成像、FRET和 FRAP可以在Zeiss元和多个软件包上执行，从而允许定量评估 所有收集的数据。2009年将安装立体学和旋转磁盘系统。不经常使用的用户可能 请求由其中一名专家工作人员提供全面服务成像。该资源积极参与 通过提供旅游、宣传校外演讲者、新用户培训、年度开放见面会等方式进行教育 以及实际操作的研讨会。使用该资源的癌症中心成员包括29个研究小组， 代表所有四个癌症中心研究项目，占总使用量的60%。增加的价值 癌症中心成员的证据来自22个公共广告、期刊封面图片(2008年)和31个外部图片 在报告期间(2009财年)使用资源的项目(12个NCI、ACS或LLS)。开创性的发现 与致癌病毒进入、G偶联受体功能、DNA损伤和修复、细胞内 信号传递、内吞作用的机制和生物技术的发展都是由该资源实现的。

As a core resource supported by NCI, the overall goals of the UNM Fluorescence Microscopy Resource are to provide: a) support services through user access to state-of-the art facilities and technologies, b) user training and individualized support or collaboration to address cancer relevant questions, c) innovation through the testing and development of new probes and imaging technologies, d) dissemination through resource tours, poster displays, an annual open house, workshops and a web page. The UNM Fluorescence Microscopy and Cell Imaging Shared Resource offers complete fluorescence microscopic imaging services including ratiometric imaging of ions, deconvolufion, confocal, hyperspectral and convenfional light microscopic imaging of live and fixed samples. Fee for service charges are in line with mid-range nation-wide rates and UNM Cancer Center members benefit from a 20% copay. The Resource is centrally located in four rooms on the second floor of the Cancer Research Facility. Instrumentafion is regularly upgraded through a mix of extramural (NCRR, NCI) and intramural (UNM SOM and Cancer Center) funding. Current instruments include upright and inverted Zeiss, Nikon and Olympus microscopes fitted with Zeiss LSM 510, Zeiss META and BioRad Radiance confocal scanheads and TillVision ratiometric system. CRi Nuance, Andor iXon, Orca and Zeiss digital cameras offer low light sensitivity and spectral image collecfion on widefield instruments. Up to eight color imaging, FRET and FRAP can be performed on the Zeiss META and mulfiple software packages allow quantitative evaluafion of all collected data. A stereology and spinning disk system will be installed in 2009. Infrequent users may request full service imaging by one of the expert staff members. The Resource is acfively involved in educafion by providing tours, hosfing extramural speakers, new user training, annual open house meefings and hands-on workshops. Cancer Center members using the Resource encompass 29 research groups, representing all four Cancer Center research programs, and account for >60% total use. Value added for Cancer Center members is evidenced by 22 publicafions, journal cover pictures (CY08) and 31 extramural projects (12 NCI, ACS or LLS) using the Resource in the reporting period (FY09). Seminal discoveries pertinent to oncogenic virus entry, G-coupled receptor function, DNA damage and repair, intracellular signaling, mechanisms of endocytosis, and biotechnology development have been enabled by the Resource.