Biomolecular Nuclear Magnetic Resonance Shared Resource

生物分子核磁共振共享资源

• 批准号: 8465118
• 负责人: MARY C. BECKERLE
• 金额: $ 4.63万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8465118
• 关键词: Advisory Committees; Arts; Biochemistry; Biological Factors; Biomolecular Nuclear Magnetic Resonance; Budgets; Cancer Center; Cancer Center Support Grant; Cell Differentiation process; Cells; Colorado; Communities; Computer software; Data; Data Collection; Developmental Therapeutics Program; Device or Instrument Development; Diagnostic Imaging; Doctor of Philosophy; Drug Delivery Systems; Faculty; Fee-for-Service Plans; Funding; Institutes; Laboratories; Leadership; Maintenance; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Methods; Molecular Structure; NMR Spectroscopy; Natural Product Drug; Nuclear; Nuclear Magnetic Resonance; Nucleic Acids; Organic Chemicals; Peer Review; Play; Polymers; Process; Proteins; Regulation; Research; Research Personnel; Resolution; Resource Sharing; Resources; Role; Sampling; Schedule; Services; Staging; Structure; Supervision; Surveys; System; Techniques; Technology; The Sun; Therapeutic; Time; Training; United States National Institutes of Health; Universities; Utah; Work; analytical method; base; cell growth; computerized data processing; drug development; innovation; instrument; instrumentation; interest; macromolecule; magnetic field; member; nucleic acid structure; polypeptide; professor; programs; repaired; research study; response; satisfaction; small molecule

The Biomolecular Nuclear Magnetic Resonance (BNMR) Shared Resource provides a broad range of NMRbased services for Huntsman Cancer Institute (HCI) members and the entire University of Utah research community. The Resource provides high-level technical support and access to state-of-the-art NMR software and hardware, including spectrometers operating at 400, 500, 600, 800, and 900 MHz for protein/nucleic acid structure determination as well as routine analytical NMR services. The first three instruments are located at the University of Utah in centralized space and the latter two instruments in Colorado-based regional system (Boulder, 800; Denver, 900). The 400 MHz spectrometer is dedicated to small molecule, organic, and natural products NMR applications. The 500 MHz and 600 MHz spectrometers were recently upgraded to increase BNMR Shared Resource capability. The 600 MHz spectrometer is the highest field spectrometer in Utah. The BNMR Shared Resource manages Sun, SGI, and LINUX workstations for data processing, analysis, and structure determination through the Colorado-based regional system. Commonly used software for NMR spectroscopy is provided, including CYANA2.1, FELIX2004, MOLMOL, NMRPIPE, PYMOL, SPARKY, VNMRJ,andXPLOR-NIH. The BNMR Shared Resource is directed by Jack Skalicky, PhD, a Research Assistant Professor in Biochemistry, who provides technical support for the most demanding NMR projects and assists researchers with data collection, processing, and analysis. The Resource has a full-time NMR technician responsible for training new users, performing scheduled maintenance, and recording small-molecule NMR experiments on the 400 and 500 MHz spectrometers. A shared hardware technician provides repairs. The Resource serves members of all HCI laboratory-based scientific Programs (Nuclear Control of Cell Growth and Differentiation; Cell Response and Regulation; Imaging, Diagnostics, and Therapeutics; and Gastrointestinal Cancers) to characterize molecules, particulariy proteins, that play roles in cancer mechanisms. In addition, characterization of small compounds, including natural products and drug-delivery polymers, contributes significantly to the developmental therapeutics activities of HCI. The BNMR Shared Resource is an institutionally managed facility with supervision from both University and HCI leadership. There is a Faculty Advisory Committee and the Resource is reviewed for user satisfaction by annual surveys. The facility is operated on a fee-for-service basis (chargeback). Use of the BNMR Shared Resource by Cancer Center members with peer-reviewed funding is 60 percent. Funds are requested from the CCSG to cover 16 percent ($42,543) of the proposed Resource budget. The BNMR Shared Resource has ample capacity for additional use by Cancer Center members.

生物分子核磁共振（BNMR）共享资源提供了广泛的基于NMR的 为亨斯迈癌症研究所（HCI）成员和整个犹他州大学的研究提供服务 社区该资源提供高水平的技术支持和最先进的NMR软件 和硬件，包括在400、500、600、800和900 MHz下运行的蛋白质/核酸光谱仪 结构测定以及常规分析NMR服务。前三种仪器位于 犹他州大学在集中的空间和后两个仪器在科罗拉多州的区域系统 （Boulder，800; Denver，900）。400 MHz光谱仪专用于小分子、有机和天然 产品NMR应用。500 MHz和600 MHz光谱仪最近进行了升级， BNMR共享资源能力。600 MHz频谱仪是犹他州最高的场频谱仪。的 BNMR共享资源管理Sun、SGI和Linux工作站，用于数据处理、分析和 通过科罗拉多州的区域系统进行结构测定。NMR常用软件 提供了光谱学，包括CYANA2.1，FELIX 2004，MOLMOL，NMRPIPE，PYMOL，SPARKY， VNMRJ和XPLOR-NIH. BNMR共享资源是由杰克Skalicky，博士，研究助理教授在 生物化学，谁提供技术支持，最苛刻的核磁共振项目，并协助研究人员 数据收集、处理和分析。该资源有一名全职核磁共振技术员，负责 培训新用户，执行定期维护，并记录小分子NMR实验， 400和500 MHz光谱仪。共享硬件技术人员提供维修。 该资源为所有HCI实验室科学计划（细胞核控制）的成员提供服务。 生长和分化;细胞反应和调节;成像、诊断和治疗;以及 胃肠道癌症）来表征在癌症中起作用的分子，特别是蛋白质 机制等此外，小化合物的表征，包括天然产物和药物递送 聚合物，显着有助于HCI的发育治疗活性。 BNMR共享资源是一个机构管理的设施，由大学和 HCI领导力。有一个教师咨询委员会和资源审查用户满意度， 年度调查。该设施以按服务收费的方式运营（按存储容量计费）。使用BNMR共享 癌症中心成员的资源与同行评审的资金是60%。请拨资金来自 CCSG支付拟议资源预算的16%（42，543美元）。BNMR共享资源具有 为癌症中心成员提供额外使用的充足容量。