Determination of Dosing Concentrations of Formulated APIs using the MTSA

项目总结(见说明)： 用于评估杀微生物剂预防艾滋病毒传播能力的体外试验通常使用与抗艾滋病毒治疗药物的开发更相关的模型，并使用不敏感的方法在感染后的短时间间隔(3-6天)量化病毒产生。为了克服这些关键的生物检测方法的缺点，我们开发了一种杀微生物剂传播和杀菌实验(MTSA)，以更灵敏和定量地评估细胞培养中存在杀菌化合物时病毒的传播。MTSA定义了完全抑制病毒在细胞培养中的传播、复制和传播所需的杀微生物剂的浓度，因此可以帮助定义在以配方杀微生物剂产品递送时在靶细胞中所需的杀微生物剂的有效浓度。鉴于难以量化提供有效产品所需的杀微生物剂产品的剂量，重要的是了解体外方法学，辅之以适当的体外和体内药代动力学和药效学评价，可能会为 开发过程，并有助于更好地确定适当的给药策略。在杀菌剂开发商中，剂量问题现在通过以下理念得到解决

PROJECT SUMMARY (See instructions): In vitro assays used to evaluate the ability of a microbicide to prevent HIV transmission typically utilize models more relevant to the development of anti-HIV therapeutic agents and quantify virus production at short time intervals following infection (3-6 days) using insensitive methodology. To mitigate the shortcomings of these critical biological assays, we have developed a microbicide transmission and sterilization assay (MTSA) to more sensitively and quantitatively evaluate virus transmission in cell culture in the presence of microbicidal compounds. The MTSA defines the concentration of the microbicide required to totally suppress the transmission, replication, and spread of virus in cell culture and may thus help define the effective concentration of the microbicide required to be present in target cells upon delivery in a formulated microbicide product. In light of the difficulty of quantifying the dose of a microbicide product that is required for the delivery of an effective product, it is important to understand how in vitro methodology, complemented with appropriate ex vivo and in vivo pharmacokinetic and pharmacodynamics evaluations, might inform the development process and serve to better define appropriate dosing strategies. Among microbicide developers, the dosing problem is now addressed through the philosophy of