Molecular Definition of Brain Circuits Controlling Addiction

描述（申请人提供）：NIDA P30“细胞类型介导成瘾行为的分子和表观遗传学研究中心”将为NIH研究人员提供最先进的资源，技术和专业知识，这将显着增强每个参与实验室的计划，并能够开发出对药物滥用机制的调查具有普遍效用的其他先进方法。为了实现这些目标，中心将分为四个核心：行政核心将组织和管理中心的所有计划和财务运作，包括与中心研究人员的外联，管理外部咨询委员会的季度在线会议，以及在现场实验期间接待中心研究人员或其工作人员。BAC基因工程和转基因靶向核心将产生、表征和分发专门的转基因系，用于对成瘾回路细胞类型的高级分子研究;分子和表观遗传学分析核心将提供专门的行为和实验服务，用于在慢性药物治疗、自我给药和停药的背景下进行TRAP翻译分析和表观遗传学作图研究;分子信息学核心将提供设施和专业知识，以帮助研究人员收集，存储和分析将从他们的微阵列，TRAPseq，表观基因组作图和ChlPseq研究中产生的大量数据。该中心还将支持少数试点项目，这些项目旨在开发新技术，以促进发现导致药物滥用的分子机制，或将现有方法扩展到迄今为止难以用现代分子遗传学方法处理的哺乳动物模型系统。 任何参与的实验室，以产生大量的专门的转基因系所需的综合遗传，分子和表观遗传分析成瘾电路;和一个中心，他们的实验室可以应用先进的方法来分析成瘾电路将显着推进他们的研究计划。

DESCRIPTION (provided by applicant): The NIDA P30 "Center for Molecular and Epigenetic Research of Cell Types Mediating Addictive Behaviors" proposed here will provide to NIH investigators state of the art resources, technologies and expertise that will significantly enhance the programs of each of the participating laboratories, and enable development of additional advanced methodologies that are of general utility for the investigation of mechanisms of drug abuse. To meet these objectives, the Center will be organized into four Cores: The Administrative Core will organize and manage all programmatic and fiscal operations of the Center, including outreach to each of the Center investigators, management of quarterly online meetings of the External Advisory Committee, and hosting of Center investigators or their staff during the conduct of on-site experiments. The BAC Recombineering and Transgenic Targeting Core will generate, characterize and distribute specialized transgenic lines for advanced molecular studies of cell types contributing to addiction circuitry; The Molecular and Epigenetic Profiling Core will provide specialized behavioral and experimental services for the conduct of TRAP translational profiling and epigenetic mapping studies in the context of chronic drug treatment, drug self-administration, and drug withdrawal; The Molecular Informatics Core will provide facilities and expertise to help investigators collect, store and analyze the very large amounts of data that will result from their microarray, TRAPseq, epigenome mapping and ChlPseq studies. The Center will also support a small number of Pilot Projects aimed at developing new technologies that will advance discovery of molecular mechanisms that contribute to drug abuse, or extension of existing approaches to mammalian model systems that have been refractory thus far to modern molecular genetic approaches The outstanding scientists participating in the Center share the belief that: it is beyond the capacity of any participating laboratory to generate the large number of specialized transgenic lines required for comprehensive genetic, molecular and epigenetic analysis of addiction circuits; and that a Center in which their laboratories can apply advanced methods for analysis of addiction circuits will significantly advance their research programs.