Molecular Definition of Brain Circuits Controlling Addiction

控制成瘾的大脑回路的分子定义

• 批准号: 8551017
• 负责人: NATHANIEL HEINTZ
• 金额: $ 137.68万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2018-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8551017
• 关键词: Addictive Behavior; Advisory Committees; Animal Model; Behavioral; Belief; Biological Models; Brain; Cells; Chronic; Cocaine; Core Facility; Data; Data Analyses; Data Set; Development; Drug abuse; Ensure; Epigenetic Process; Event; Funding; Gene Expression Alteration; Generations; Genetic Models; Individual; Informatics; Investigation; Laboratories; Maps; Mediating; Methodology; Methods; Molecular; Molecular Analysis; Molecular Genetics; National Institute of Drug Abuse; Nature; Nicotine; Opioid; Pharmaceutical Preparations; Pilot Projects; Refractory; Research; Research Personnel; Resources; Role; Scientist; Self Administration; Services; Site; Stimulus; Substance abuse problem; Techniques; Technology; Training; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; Withdrawal; addiction; cell type; comparative; computing resources; drug of abuse; drug withdrawal; epigenome; in vivo; investigator training; meetings; neural circuit; new technology; novel strategies; novel therapeutic intervention; operation; outreach; programs; public health relevance; ranpirnase; recombinase; research study; response; technology development

DESCRIPTION (provided by applicant): The NIDA P30 "Center for Molecular and Epigenetic Research of Cell Types Mediating Addictive Behaviors" proposed here will provide to NIH investigators state of the art resources, technologies and expertise that will significantly enhance the programs of each of the participating laboratories, and enable development of additional advanced methodologies that are of general utility for the investigation of mechanisms of drug abuse. To meet these objectives, the Center will be organized into four Cores: The Administrative Core will organize and manage all programmatic and fiscal operations of the Center, including outreach to each of the Center investigators, management of quarterly online meetings of the External Advisory Committee, and hosting of Center investigators or their staff during the conduct of on-site experiments. The BAC Recombineering and Transgenic Targeting Core will generate, characterize and distribute specialized transgenic lines for advanced molecular studies of cell types contributing to addiction circuitry; The Molecular and Epigenetic Profiling Core will provide specialized behavioral and experimental services for the conduct of TRAP translational profiling and epigenetic mapping studies in the context of chronic drug treatment, drug self-administration, and drug withdrawal; The Molecular Informatics Core will provide facilities and expertise to help investigators collect, store and analyze the very large amounts of data that will result from their microarray, TRAPseq, epigenome mapping and ChlPseq studies. The Center will also support a small number of Pilot Projects aimed at developing new technologies that will advance discovery of molecular mechanisms that contribute to drug abuse, or extension of existing approaches to mammalian model systems that have been refractory thus far to modern molecular genetic approaches The outstanding scientists participating in the Center share the belief that: it is beyond the capacity of any participating laboratory to generate the large number of specialized transgenic lines required for comprehensive genetic, molecular and epigenetic analysis of addiction circuits; and that a Center in which their laboratories can apply advanced methods for analysis of addiction circuits will significantly advance their research programs.

描述(由申请人提供)：NIDA P30“介导成瘾行为的细胞类型的分子和表观遗传学研究中心”将为NIH的研究人员提供最先进的资源、技术和专业知识，这些资源、技术和专业知识将显著增强每个参与实验室的计划，并使更多的先进方法得以开发，这些方法对药物滥用机制的调查具有普遍意义。为实现这些目标，中心将分为四个核心：行政核心将组织和管理中心的所有方案和财政业务，包括与中心的每一名调查员进行接触，管理外部咨询委员会的季度在线会议，以及在进行现场试验期间接待中心的调查员或其工作人员。BAC重组工程和转基因靶向核心将产生、鉴定和分发专门的转基因品系，用于促进成瘾回路的细胞类型的高级分子研究；分子和表观遗传图谱核心将为在慢性药物治疗、药物自我给药和药物戒断背景下进行TRAP翻译图谱和表观遗传学图谱研究提供专门的行为和实验服务；分子信息学核心将提供设施和专业知识，帮助研究人员收集、存储和分析他们的微阵列、TRAPseq、表观基因组图谱和ChlPseq研究产生的大量数据。该中心还将支持少数旨在开发新技术的试点项目，这些新技术将促进发现导致药物滥用的分子机制，或扩展到哺乳动物模型系统的现有方法，这些方法迄今对现代分子遗传学方法难以实现。参与该中心的杰出科学家都认为：它超出了能力范围。 任何参与的实验室都有能力产生大量专门的转基因品系，对成瘾回路进行全面的遗传、分子和表观遗传学分析；他们的实验室可以在其中应用先进方法分析成瘾回路的中心将大大推进他们的研究计划。