GENE EXPRESSION NERVOUS SYSTEM ATLAS (GENSAT)

该合同的总体目标是：1）继续对小鼠神经系统发育过程中的基因表达模式进行高通量分析; 2）为科学界提供新的小鼠遗传研究工具。该合同使用两种互补和协调的技术（标准放射性原位杂交和BAC转基因报告小鼠），筛选大量探针（例如，数千）的基因产物在相对有限数量（10至20）的神经系统部分上。截面平面由NINDS选择，以包括神经科学界最普遍感兴趣的结构（例如，新皮层、基底神经节、海马、小脑、脊髓等）。在发育的3或4个阶段（早期和晚期胚胎，以及出生后早期）和成年小鼠中分析基因表达的空间位置/模式。由此产生的基因表达图像被数字化并发布在网络可访问的数据库中，所有由合同产生的BAC转基因小鼠都被存放在NIH赞助的小鼠库中。

The overall objective of this contract is to 1) continue the high-throughput analysis of gene expression patterns in the mouse nervous system during development; 2) generate new mouse genetic research tools for the scientific community. Using two complementary and coordinated technologies (standard radiometric in situ hybridization and BAC transgenic reporter mice), the contract screens probes for a large number (e.g., thousands) of gene products on a relatively limited number (10 to 20) of sections of the nervous system. Planes of section are chosen by the NINDS to include the structures of most general interest to the neuroscience community (e.g., neocortex, basal ganglia, hippocampus, cerebellum, spinal cord, etc.). The spatial locations/patterns of gene expression are analyzed at 3 or 4 stages of development (early and late embryonic, and early postnatal) and in adult mice. The resulting images of gene expression are digitized and posted in Web-accessible databases, and all BAC transgenic mice generated by the contract are deposited in an NIH-sponsored mouse repository.