Lectimers: Glycan-anchored scaffold libraries for targeting carbohydrate-binding

Lectimers：用于靶向碳水化合物结合的聚糖锚定支架文库

• 批准号: 8624937
• 负责人: MATTHEW LEVY
• 金额: $ 27.72万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-20 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8624937
• 关键词: Affinity; Avidity; Binding; Biological Markers; Biological Process; Carbohydrates; Cells; Chemicals; DNA; DNA biosynthesis; Development; Diagnostic; Disaccharides; Disease; Ensure; Galactose Binding Lectin; Galectin 1; Galectin 3; Human; Hydroxyl Radical; Individual; Lectin; Libraries; Ligands; Malignant Neoplasms; Methodology; Methods; Modification; Monosaccharides; Nucleic Acids; Peptides; Phase; Play; Polymerase; Polysaccharides; Positioning Attribute; Protein Binding; Protein Family; Proteins; Reading; Reagent; Role; Solid; Specificity; System; Technology; Therapeutic; Therapeutic Human Experimentation; Time; Ursidae Family; Variant; Vertebral column; Virus Diseases; base; cancer type; carbohydrate binding protein; chemical synthesis; cost; flexibility; fluorophore; functional group; human disease; immunoregulation; member; monomer; next generation sequencing; novel; phosphoramidite; population based; public health relevance; scaffold; small molecule; statistics; sugar; tumorigenesis

DESCRIPTION (provided by applicant): We propose to develop a novel platform technology that will produce high-affinity and high-specificity ligands to target carbohydrate binding protein (CBPs), a diverse class of proteins which possess a wide range of biological functions, playing roles cancer tumorigeneisis, immune modulation and viral infection. Our method builds upon advances in sequencing technologies and leverages the natural, low affinity, interactions of monovalent sugars for CBPs combined with the ease of synthesis of nucleic acids. Our glycan-anchored libraries will thus possess many of the attributes of nucleic acids - in particular structural rigidity, ease of synthesis and the ease with which they can be amplified and characterized - but with the enhanced chemical functionality observed in peptides, proteins and small molecule ligands. Because these reagents are based on nucleic acids, the resulting affinity agents can be readily synthesized at relatively low cost and can be easily modified with a variety of fluorophores or chemical moieties for diagnostic, therapeutic and research purposes.

描述（由申请人提供）：我们提议开发一种新型平台技术，该技术将产生高亲和力和高特异性的配体来靶向碳水化合物结合蛋白（CBP），这是一类具有广泛生物学功能的蛋白质，在癌症中发挥作用肿瘤发生、免疫调节和病毒感染。我们的方法建立在测序技术的进步基础上，并利用了天然的、低亲和力的单价糖与CBP的相互作用以及核酸合成的便利性。因此，我们的聚糖锚定文库将具有核酸的许多属性-特别是结构刚性，易于合成以及易于扩增和表征-但具有在肽，蛋白质和小分子配体中观察到的增强的化学功能。因为这些试剂是基于核酸的，所以所得亲和剂可以以相对低的成本容易地合成，并且可以容易地用亲和剂修饰。 用于诊断、治疗和研究目的的各种荧光团或化学部分。