Targeting Cancer Cells with Functionalized Nanoparticle Libraries

用功能化纳米粒子库靶向癌细胞

• 批准号: 8504814
• 负责人: MATTHEW LEVY
• 金额: $ 16.55万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-12 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8504814
• 关键词: Affinity; Animal Model; Avidity; Binding; Biological; Cancer Diagnostics; Capsid Proteins; Cells; Detection; Development; Diagnostic; Disease; Engineering; Evolution; Generations; Goals; Human; In Vitro; Individual; Lead; Libraries; Ligands; Magnetic Resonance Imaging; Magnetism; Methodology; Methods; Microspheres; Modeling; Monitor; Mus; Nucleic Acids; Population; Property; Proteins; Research; Screening for cancer; Serum; Signal Transduction; Surface; System; Techniques; Technology; Testing; Therapeutic; Therapeutic Human Experimentation; Time; Virus; Work; Xenograft procedure; aptamer; base; cancer cell; cancer therapy; nanoparticle; novel; particle; prostate cancer cell; tissue culture; tumor; tumor xenograft

DESCRIPTION (provided by applicant): We propose to use a combination of two powerful techniques: aptamer selection (systematic evolution of ligands by exponential enrichment; SELEX) and in vitro compartmentalization (IVC), to develop a new method of generating libraries of functionalized nanoparticles and that can be screened directly for function. While the goal of this application is to develop a novel system for the direct identification of targeted nanoparticles which localize to human tumors, we will develop a platform technology which extends beyond the detection and treatment of cancer and other diseases and will result in the generation of a novel class of capture agents that will find use in a variety of applications and fields of research.

描述(申请人提供)：我们建议结合使用两种强大的技术：适体选择(通过指数富集法对配体的系统进化；SELEX)和体外隔室(IVC)，以开发一种新的方法来生成功能化纳米粒的文库，并可以直接从功能上进行筛选。虽然这项应用的目标是开发一种新的系统，用于直接识别定位于人类肿瘤的靶向纳米颗粒，但我们将开发一种平台技术，其范围超出癌症和其他疾病的检测和治疗，并将导致一种新型捕获剂的产生，该捕获剂将在各种应用和研究领域中使用。

项目成果

An RNA alternative to human transferrin: a new tool for targeting human cells.

• DOI: 10.1038/mtna.2012.14
• 发表时间: 2012-05-15
• 期刊: Molecular therapy. Nucleic acids