Role of the cellular microRNA, miR-155, in EBV type III latency signaling

细胞 microRNA miR-155 在 EBV III 型潜伏信号传导中的作用

• 批准号: 8455707
• 负责人: ERIK K FLEMINGTON
• 金额: $ 28.19万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8455707
• 关键词: Acquired Immunodeficiency Syndrome; Address; Apoptosis; B-Cell Activation; B-Cell Lymphomas; Biology; Burkitt Lymphoma; Cells; Cloning; Data Analyses; Development; Elements; Epstein-Barr Virus latency; Gene Expression; Gene Targeting; Genes; Genetic; Growth; Herpesviridae; Hodgkin Disease; Homologous Gene; Human; Human Herpesvirus 4; Immune; Individual; Kaposi Sarcoma; Knock-out; Malignant Neoplasms; Mediating; MicroRNAs; Mus; Mutation; Nasopharynx Carcinoma; Non-Hodgkin' s Lymphoma; Oncogenic; Paper; Patients; Play; Population; Predisposition; Probability; Proteins; Reagent; Role; Signal Transduction; Signal Transduction Pathway; Transforming Growth Factor beta; Untranslated Regions; Validation; base; cancer cell; inhibitor/antagonist; neoplastic cell; programs; response; tumorigenesis

The Epstein Barr virus (EBV) is an oncogenic herpesvirus that is intimately involved in a number of malignancies in humans. The genetic basis of EBV associated oncogenesis is the concerted action of EBV latency associated genes and varying cellular genetic alterations. In immuno-competent individuals only minimal EBV latency gene expression can be tolerated due to the immunogeneticity of several EBV encoded latency gene products. In AIDS patients, however, expression of the full repertoire of latency genes (referred to as type III latency) can sometimes be tolerated and expression of these genes provide many essential elements of tumor cell development. In this setting, fewer cellular genetic alterations are required to give rise to malignant cell populations and this probably partly explains the greatly increased susceptibility of AIDS patients to EBV associated non-Hodgkin's lymphomas. The cellular microRNA, miR-155, is one of the most highly implicated microRNAs in cancer. miR-155 is induced by the EBV type III latency program (but not the type I latency program) suggesting a possible role for miR-155 in modulating type III latency signal transduction. Further evidence that miR-155 signaling is relevant to herpesvirus biology has been provided by Rolf Renne's lab and by Bryan Cullen's lab who both showed recently that the Kaposi's Sarcoma Herpes virus (KSHV) encodes a functional homologue of miR- 155. Two mouse miR-155 knock out papers recently showed that miR-155 is important for B cell activation responses following immune challenge. We hypothesize that induction of miR-155 by EBV type III latency plays a role in facilitating EBV mediated B cell activation and that miR-155 modulates signal transduction pathways that contribute to EBV associated maligancies in AIDS patients.

eb病毒（EBV）是一种致癌的疱疹病毒，与许多疾病密切相关

项目成果

miRNA-mRNA correlation-network modules in human prostate cancer and the differences between primary and metastatic tumor subtypes.

• DOI: 10.1371/journal.pone.0040130
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Zhang W;Edwards A;Fan W;Flemington EK;Zhang K
• 通讯作者: Zhang K

Expanding the conversation on high-throughput virome sequencing standards to include consideration of microbial contamination sources.

扩大有关高通量病毒组测序标准的讨论，以纳入对微生物污染源的考虑。

• DOI: 10.1128/mbio.01989-14
• 发表时间: 2014
• 期刊: mBio
• 影响因子: 6.4
• 作者: Strong,MichaelJ;Lin,Zhen;Flemington,ErikK
• 通讯作者: Flemington,ErikK

Epstein - Barr virus Latent Membrane Protein 1 suppresses reporter activity through modulation of promyelocytic leukemia protein-nuclear bodies.

Epstein-Barr 病毒潜伏膜蛋白 1 通过调节早幼粒细胞白血病蛋白核体来抑制报告基因活性。

• DOI: 10.1186/1743-422x-8-461
• 发表时间: 2011
• 期刊: Virology journal
• 影响因子: 4.8
• 作者: Sides,MarkD;Block,GregoryJ;Chadwick,ReidW;Shan,Bin;Flemington,ErikK;Lasky,JosephA
• 通讯作者: Lasky,JosephA

Microbial contamination in next generation sequencing: implications for sequence-based analysis of clinical samples.

• DOI: 10.1371/journal.ppat.1004437
• 发表时间: 2014-11
• 期刊: PLoS pathogens
• 影响因子: 6.7
• 作者: Strong MJ;Xu G;Morici L;Splinter Bon-Durant S;Baddoo M;Lin Z;Fewell C;Taylor CM;Flemington EK
• 通讯作者: Flemington EK

The sequence structures of human microRNA molecules and their implications.

• DOI: 10.1371/journal.pone.0054215
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Fang Z;Du R;Edwards A;Flemington EK;Zhang K
• 通讯作者: Zhang K