Multifunctional Scaffold Agitation Platform

多功能支架搅拌平台

• 批准号: 8455519
• 负责人: TODD A MCADAMS
• 金额: $ 15.28万
• 依托单位: RESODYN CORPORATION
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-04-01 至 2014-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8455519
• 关键词: Acoustics; Agitation; Biological Assay; Biomass; Capital; Cell Culture Techniques; Cells; Complex; Cost Savings; Cultured Cells; Custom; Data; Devices; Digestion; Effectiveness; Environment; Equipment; Frequencies; Goals; Growth; High temperature of physical object; Hydration status; Infusion Pumps; Investments; Liquid substance; Methods; Movement; Nutrient; Outcome; Oxygen; Patients; Penetration; Performance; Perfusion; Phase; Process; Property; Pump; Recovery; Research Proposals; Seeds; Small Business Innovation Research Grant; Solutions; Stream; System; Techniques; Technology; Therapeutic; Time; Tissue Engineering; Tissues; Translating; Ultrasonography; Vacuum; Vision; Work; base; cell growth; cell injury; cell type; commercial application; cost; culture plates; design; flasks; fluid flow; improved; novel; novel strategies; operation; prototype; public health relevance; rapid technique; research study; restraint; scaffold; sound frequency; tissue support frame; tool

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I project will develop a commercially-viable device that uses micro-scale acoustic streaming (low frequency sound energy) to deliver mixing to the interior of three-dimensional (3D) scaffolds used for tissue engineering and other applications. The enabling advantage of low frequency sound energy is the ability to generate micro-scale mixing in and around scaffolds that can enhance the movement of liquid, molecules, and oxygen within scaffolds without the need for pumps or a costly and inconvenient perfusion apparatus for each scaffold. Preliminary data shows that cells can successfully grow in the presence of the acoustic energy field. Nutrient supply issues and difficulties in homogenously seeding dense tissue engineering scaffolds are issues that need to be overcome in order to successfully produce high quality, repeatable cell cultures in the complex 3D environments that are the mainstay of modern tissue engineering. Many possible solutions to these problems have been examined, including the use of spinner flask, centrifugal, vacuum fixtures, or perfusion for cell seeding. The vision for the approach proposed here is to develop a single agitation platform on which multiple scaffolds can be mounted in well-plate fixtures to deliver similar benefits as those derived from the perfusion approach but without the significant time and capital investment inherent in the perfusion loop approach. When used at low intensities, the proposed device should be able to deliver benefits similar to a perfusion approach, but in a much simpler package. When used at higher intensities in the absence of cells, additional applications and benefits can be delivered in the form of much more rapid methods for the hydration, functionalization, and enzymatic degradation of scaffolds. The goal of this research proposal is to build a robust working prototype device and to evaluate its utility as a very simple solution for the enhancement of scaffold-based cell cultures. The commercial applications of the device would be much broad within the field of tissue engineering, extending to the full range of tissues that have shown to be enhanced by more cumbersome perfusion flow-based systems, as well as to basic operations such as hydrating, functionalizing, and digesting scaffolds.

描述（由申请人提供）：该小型企业创新研究第一阶段项目将开发一种商业上可行的设备，该设备使用微尺度声流（低频声能）将混合传递到用于组织工程和其他应用的三维（3D）支架的内部。低频声能的有利优点是能够在支架内和周围产生微尺度混合，这可以增强液体、分子和氧气在支架内的运动，而不需要用于每个支架的泵或昂贵且不方便的灌注设备。初步数据表明，细胞可以在声能场的存在下成功生长。 营养供应问题和均匀接种致密组织工程支架的困难是需要克服的问题，以便在复杂的3D环境中成功生产高质量，可重复的细胞培养物，这是现代组织工程的支柱。许多可能的解决方案，这些问题已经检查，包括使用转瓶，离心，真空夹具，或灌注细胞接种。这里提出的方法的愿景是开发一个单一的搅拌平台，在该平台上可以将多个支架安装在孔板固定装置中，以提供与灌注方法类似的益处，但没有灌注回路方法中固有的大量时间和资本投资。当在低强度下使用时，所提出的设备应该能够提供类似于灌注方法的益处，但包装要简单得多。当在不存在细胞的情况下以更高强度使用时，可以以用于支架的水合、功能化和酶促降解的更快速方法的形式提供额外的应用和益处。 本研究提案的目标是建立一个强大的工作原型设备，并评估其效用作为一个非常简单的解决方案，以增强基于支架的细胞培养。该装置的商业应用将在组织工程领域内广泛得多，延伸到已显示通过更繁琐的基于灌注流的系统增强的全范围组织，以及基本操作，如水合、功能化和消化支架。