Multifunctional Scaffold Agitation Platform

多功能支架搅拌平台

• 批准号: 8455519
• 负责人: TODD A MCADAMS
• 金额: $ 15.28万
• 依托单位: RESODYN CORPORATION
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-04-01 至 2014-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8455519
• 关键词: Acoustics; Agitation; Biological Assay; Biomass; Capital; Cell Culture Techniques; Cells; Complex; Cost Savings; Cultured Cells; Custom; Data; Devices; Digestion; Effectiveness; Environment; Equipment; Frequencies; Goals; Growth; High temperature of physical object; Hydration status; Infusion Pumps; Investments; Liquid substance; Methods; Movement; Nutrient; Outcome; Oxygen; Patients; Penetration; Performance; Perfusion; Phase; Process; Property; Pump; Recovery; Research Proposals; Seeds; Small Business Innovation Research Grant; Solutions; Stream; System; Techniques; Technology; Therapeutic; Time; Tissue Engineering; Tissues; Translating; Ultrasonography; Vacuum; Vision; Work; base; cell growth; cell injury; cell type; commercial application; cost; culture plates; design; flasks; fluid flow; improved; novel; novel strategies; operation; prototype; public health relevance; rapid technique; research study; restraint; scaffold; sound frequency; tissue support frame; tool

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I project will develop a commercially-viable device that uses micro-scale acoustic streaming (low frequency sound energy) to deliver mixing to the interior of three-dimensional (3D) scaffolds used for tissue engineering and other applications. The enabling advantage of low frequency sound energy is the ability to generate micro-scale mixing in and around scaffolds that can enhance the movement of liquid, molecules, and oxygen within scaffolds without the need for pumps or a costly and inconvenient perfusion apparatus for each scaffold. Preliminary data shows that cells can successfully grow in the presence of the acoustic energy field. Nutrient supply issues and difficulties in homogenously seeding dense tissue engineering scaffolds are issues that need to be overcome in order to successfully produce high quality, repeatable cell cultures in the complex 3D environments that are the mainstay of modern tissue engineering. Many possible solutions to these problems have been examined, including the use of spinner flask, centrifugal, vacuum fixtures, or perfusion for cell seeding. The vision for the approach proposed here is to develop a single agitation platform on which multiple scaffolds can be mounted in well-plate fixtures to deliver similar benefits as those derived from the perfusion approach but without the significant time and capital investment inherent in the perfusion loop approach. When used at low intensities, the proposed device should be able to deliver benefits similar to a perfusion approach, but in a much simpler package. When used at higher intensities in the absence of cells, additional applications and benefits can be delivered in the form of much more rapid methods for the hydration, functionalization, and enzymatic degradation of scaffolds. The goal of this research proposal is to build a robust working prototype device and to evaluate its utility as a very simple solution for the enhancement of scaffold-based cell cultures. The commercial applications of the device would be much broad within the field of tissue engineering, extending to the full range of tissues that have shown to be enhanced by more cumbersome perfusion flow-based systems, as well as to basic operations such as hydrating, functionalizing, and digesting scaffolds.

描述（由申请人提供）：这个小型企业创新研究阶段I项目将开发一种可使用微型声学流（低频声能）的商业可行的设备，以将混合使用用于组织工程和其他应用的三维（3D）支架的内部。低频声音能量的能力是能够在支架内和周围产生微尺度混合，从而可以增强脚手架内液体，分子和氧气的运动，而无需泵，每种支架的昂贵和不便的灌注设备。初步数据表明，在声学场的存在下，细胞可以成功生长。 营养供应问题和同质播种致密组织工程支架的困难是需要克服的问题，以便在复杂的3D环境中成功产生高质量的可重复的细胞培养，这是现代组织工程的主要支柱。已经检查了许多解决这些问题的解决方案，包括使用旋转器瓶，离心，真空固定装置或用于细胞播种的灌注。此处提出的方法的愿景是开发一个单个搅动平台，可以将多个脚手架安装在固定装置中，以提供与灌注方法所获得的相似的好处，但没有灌注环方法中固有的大量时间和资本投资。当以低强度使用时，建议的设备应能够提供类似于灌注方法的收益，但要简单得多。如果在没有细胞的情况下以较高的强度使用时，可以以更快的方法的形式传递其他应用和益处，以进行水化，功能化和酶促降解脚手架的降解。 这项研究建议的目的是建立一个强大的工作原型设备，并评估其用途作为一种非常简单的解决方案，以增强基于脚手架的细胞培养物。该设备的商业应用将在组织工程领域范围很广，扩展到已显示出更繁琐的基于灌注流的系统以及基本操作（例如水化，功能化和消化脚手架）增强的整个组织。