Taxol Production with a Novel Fermentation Reactor

使用新型发酵反应器生产紫杉醇

• 批准号: 6483793
• 负责人: TODD A MCADAMS
• 金额: $ 13.57万
• 依托单位: RESODYN CORPORATION
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2004-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6483793
• 关键词: Moniliales; bioreactors; biosynthesis; biotechnology; drug design /synthesis /production; fermentation; mass tissue /cell culture; method development; paclitaxel

DESCRIPTION (provided by applicant): The specific innovation proposed for this Phase I project will combine an advanced, novel low-frequency sonic energy technology with the production of fungal secondary metabolites of pharmaceutical importance. The Phase 1 project will demonstrate the use of a novel fermentation reactor to provide improved agitation and aeration during the in vitro cultivation of the endophytic fungus, Penicililum raistrickii, to increase the expression of Taxol. It is proposed to optimize the expression of Taxol by manipulating the fermentation medium and exploiting the unique features of the low frequency sonic technology to ensure effective renewal of the critical medium components to the cell membrane. The impact of the carbohydrate source, nitrogen and phosphate control, addition of pre-cursors, as well as triggers such as yew tree extract and rival fungal extracts, will be investigated. The ultimate objective is a commercially viable route to Taxol based on an optimized strain of Penicillium raistrickii, an optimized fermentation protocol and a high-intensity, low shear fermentation reactor. The overall technical target is to achieve Taxol expression of 10 mg/I.

描述（由申请人提供）：为此提出的具体创新 一期工程将结合联合收割机，一种先进的，新颖的低频声能 真菌次级代谢产物的生产技术 药物的重要性。第一阶段项目将展示使用 提供改进的搅拌和通气的新型发酵反应器 内生真菌Penicilium raistrickii的体外培养， 增加紫杉醇的表达。建议优化的表达， 紫杉醇通过操纵发酵培养基和开发独特的 低频声波技术的特点，以确保有效的更新， 关键的培养基成分到细胞膜上。的影响 碳水化合物源，氮和磷酸盐控制，前体的添加， 以及紫杉树提取物和竞争对手的真菌提取物等触发物， 研究了最终目标是找到一条商业上可行的紫杉醇生产路线 以一株优化的雷氏青霉菌为基础， 发酵方案和高强度、低剪切发酵反应器。的 总的技术目标是实现10 mg/l的紫杉醇表达。