Resource for the Development of Biomedical Accelerator Mass Spectrometry (AMS)

国家加速器质谱学资源(AMS)成立于1999年，旨在实现 生物医学研究人员在探索基本原理的同时准确量化极低水平的放射性同位素 生物学中的问题。在这次更新中，我们将通过开发完全集成的 高效液相色谱AMS，以提高我们的代谢测量能力，这是我们的合作者所需要的。我们会 发展研究生化途径和细胞过程的方法，低至单细胞水平。 最后，我们将开发和验证AMS在人类翻译研究中的应用方法 这是合作者和服务用户日益增长的需求领域。在整个赠款期限内 我们将继续为研究界提供资源，包括为调查人员提供服务 熟悉AMS，培训调查人员的技术和资源的传播。朝向 这些目标，我们的具体目标是： 1)通过直接连接到分离仪器来增加AMS的吞吐量。 2.)通过将分子同一性与AMS相结合来增加AMS测量的价值和信息含量 从非常小的细胞、动物和人类样本中定量确定用于通径分析的分离株。 3.)使用取样材料中的多个同位素示踪剂对生物系统进行定量。 4.)为协作型和服务型客户提供高吞吐量的精确量化。

The National Resource for Accelerator Mass Spectrometry (AMS) was established in 1999 to enable biomedical researchers to accurately quantify very low levels of radioisotopes while exploring fundamental issues in biology. In this renewal, we will expand our present capabilities by developing a fully integrated HPLC AMS to increase our capabilities for metabolic measurements which our collaborators require. We will develop methods to study biochemical pathways and cellular processes down to the level of the single cell. Finally we will develop and validate methods for the application of AMS in human translational research which is a growing area of demand by collaborators and service users. Throughout the tenure of the grant we will continue to provide a resource to the research community that will include service to investigators familiar with AMS, training of investigators in the technology and dissemination of the Resource. Towards these goals, our specific aims are to: 1.) Increased throughput of AMS through direct coupling to separatory instruments. 2.) Increase the value and information content of AMS measurements by combining molecular identities with quantitation of defined isolates for pathway analysis from very small cellular, animal, and human samples. 3.) Provide quantitation of biological systems using multiple isotopic tracers within sampled materials. 4.) Provide high throughput precision quantitation for collaborative and service clients.