Development of laser spectroscopic methods for quantification of 14C

描述(申请人提供)：我们建议开发一种桌面大小的光谱方法来量化毫克大小的生物样品中的14C，直到自然同位素丰度的水平，使用一种名为腔振铃光谱的技术。这一系统的获取和操作成本将大大降低，并最终可能取代目前最先进的技术--加速器质谱仪。这一方法将导致14C分析的新范式，使14C标记的治疗实体的代谢、处置、命运和质量平衡能够进行研究，用于药物开发的潜在毒物，用于风险评估的潜在毒物，以及用于个性化药物的生物标志物的分析。考虑到预期的成本和操作的简单性，这项新技术将比目前可用的更多地获得高灵敏度的14C分析，因为任何生物医学或生物化学实验室都负担得起操作这种仪器的费用。

DESCRIPTION (provided by applicant): We propose to develop a table-top sized spectroscopic method to quantify 14C in milligram sized biological samples at levels down to the natural isotopic abundance, using a technique called Cavity Ring Down Spectroscopy. This system will be much cheaper to acquire and to operate, and could eventually replace the current state of the art technology -- accelerator mass spectrometry. This approach will lead to a new paradigm for 14C analysis in allowing studies to be carried out on the metabolism, disposition, fate and mass balance of 14C-labeled therapeutic entities for drug development, potential toxicants for risk assessment, and analysis of biomarkers for personalized medicine. Given the expected cost and simplicity of operation, this new technique will enable much greater access to high sensitivity 14C analysis than now currently available as any biomedical or biochemistry laboratory could afford to operate such an instrument.