Proteomics

蛋白质组学

• 批准号: 8567318
• 负责人: KARI B Basso
• 金额: $ 10.08万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8567318
• 关键词: Accounting; Address; Agreement; Area; Back; Basic Science; Bioinformatics; Biological Assay; Biological Markers; Cancer Center Support Grant; Charge; Chemicals; Church; Clinical Investigator; Complex; Complex Mixtures; Diagnosis; Doctor of Philosophy; Environment; Equipment; Evaluation; Experimental Designs; Fees; Fractionation; Funding; Goals; High Pressure Liquid Chromatography; Image; Image Analysis; Instruction; Leadership; Link; Maintenance; Malignant Neoplasms; Mass Spectrum Analysis; Methods; Mission; Post Translational Modification Analysis; Post-Translational Protein Processing; Preparation; Problem Solving; Productivity; Protein Analysis; Proteins; Proteomics; Proteomics Shared Resource; Regimen; Research; Research Personnel; Research Project Grants; Resource Sharing; Sampling; Services; Solid; System; Technical Expertise; Technology; Therapeutic; Training; Training and Education; Two-Dimensional Polyacrylamide Gel Electrophoresis; Wages; advanced system; anticancer research; base; biomedical informatics; cost; cost effective; data mining; design; experience; gel electrophoresis; informatics shared resource; innovation; instrumentation; mass spectrometer; member; operation; programs; protein complex; protein expression; protein protein interaction; research study; tandem mass spectrometry; tool

The mission of the OSUCCC Proteomics Shared Resource (PSR) is to provide instrumentation, scientific expertise and shared research services for the identification of proteins, protein modifications and protein biomarkers to advance the productivity of cancer research of OSUCCC members at cost effective rates. High-quality proteomic analysis requires sophisticated protein separation approaches, image analysis capabilities, a variety of mass spectrometric systems, and advanced bioinformatics capabilities. The PSR is designed to provide affordable and high-quality service in each of these areas. Experienced PSR staff members provide effective services to identify proteins or protein complexes using the current state-of-theart mass spectrometers and supporting equipment Outstanding institutional support and value-added partnerships allow the PSR to offer a complete range of equipment and expertise for ID PAGE, 2D PAGE, multiplex analysis and differential image gel electrophoresis (DIGE), protein complex and post-translational modification analysis and other experimental approaches requiring protein identification. These services are linked to other key OSUCCC Shared Resources such as the Biomedical Informatics Shared Resource as well as PSR-supported bioinformatic tools required for data mining, including Mascot on a 16-node cluster. Kari Green-Church, Ph.D., and a team of highly-trained staff provide established scientific leadership for PSR to give OSUCCC cancer investigators effective technical expertise for proteomic analysis. Consultative services guide OSUCCC researchers seeking to address research questions from chemical and protein biomarker determination to unraveling complex protein-protein interactions from basic science experiments. Since 2004, over $4.0 million of institutional support has been invested in the operation and expansion of the PSR, providing staff salaries and cost matches for new instrumentation User fees generated are effectively leveraged for the operation costs (maintenance agreements, supplies and upgrades). The PSR has shown increased usage by OSUCCC members, now exceeding 48 members representing five of six OSUCCC Scientific Programs. OSUCCC members account for more than 56% of total usage of the PSR. This is a solid increase from 2004 when approximately 30 OSUCCC members used the shared resource and only constituted less than 30% of total usage. Collectively, PSR supports a wide range of services from standard proteomic assays to innovative consultative expertise to enhance cancer-relevant studies. It offers cancer investigators the critical tools and expertise needed for identifying proteins, quantifying protein expression levels, discovery of protein modifications and protein biomarkers We now request CCSG funding forthe PSR that will provide 19.4% of the total support for this important full Shared Resource over the next five years.

OSUCCC蛋白质组学共享资源(PSR)的使命是为识别蛋白质、蛋白质修饰和蛋白质生物标志物提供仪器、科学专业知识和共享研究服务，以成本效益的速度提高OSUCCC成员的癌症研究生产率。 高质量的蛋白质组分析需要复杂的蛋白质分离方法、图像分析能力、各种质谱学系统和先进的生物信息学能力。PSR旨在为上述每个领域提供负担得起的高质量服务。经验丰富的PSR工作人员使用当前最先进的技术提供有效的服务来识别蛋白质或蛋白质复合体 质谱仪和辅助设备杰出的机构支持和增值合作伙伴关系使PSR能够为ID PAGE、2D PAGE、多重分析和差示图像凝胶电泳(DGE)、蛋白质复合体和翻译后修饰分析以及其他需要蛋白质鉴定的实验方法提供全套设备和专业知识。这些服务 链接到其他关键的OSUCCC共享资源，如生物医学信息学共享资源以及PSR支持的数据挖掘所需的生物信息工具，包括16节点集群上的Mascot。卡里·格林-丘奇博士和一支训练有素的团队为PSR提供了公认的科学领导地位，为OSUCCC癌症研究人员提供了蛋白质组分析的有效技术专长。咨询服务指导OSUCCC研究人员寻求解决来自 从基础科学实验中解开复杂蛋白质-蛋白质相互作用的化学和蛋白质生物标志物的测定。自2004年以来，为PSR的运作和扩建投入了400多万美元的机构支助，为新仪器产生的工作人员工资和费用匹配有效地利用了运作费用(维护协议、用品 和升级)。PSR显示OSUCCC成员的使用量增加，目前已超过48名成员，代表OSUCCC六个科学项目中的五个。OSUCCC成员占PSR总使用量的56%以上。与2004年相比，这是一个稳健的增长，当时约有30个OSUCCC成员使用共享资源，仅占总使用量的不到30%。总体而言，PSR支持 广泛的服务，从标准的蛋白质组分析到创新的咨询专业知识，以加强与癌症相关的研究。它为癌症研究人员提供了识别蛋白质、量化蛋白质表达水平、发现蛋白质修饰和蛋白质生物标记物所需的关键工具和专业知识 我们现在请求CCSG为PSR提供资金，这将在未来五年为这一重要的完全共享资源提供19.4%的总支持。