Sonic Hedgehog Dependent Neuron-Astrocyte Crosstalk During Cortical Circuit Assembly

项目摘要 神经元-神经胶质关系在突触形成和神经元的调节中起着关键作用 规格。神经元和星形胶质细胞通过细胞和分子机制 不能很好地理解。这项计划中的实验将揭示分子和细胞 神经源性SHH蛋白协调皮质星形胶质细胞行为的机制 促进大脑中神经回路的形成和功能。在我们的研究中，我们利用大的 补充小鼠遗传工具，使我们能够专门操纵星形胶质细胞中的Shh信号。vbl.使用 尖端的光学和电子显微镜方法，我们将定义 星形胶质细胞和神经元依赖Shh信号，并评估星形胶质细胞如何影响发育 功能电路。总而言之，这些目标将提供一个框架来理解交付、接收 以及发育中的皮质对SHH的细胞反应。

Project Summary Neuronal-glial relationships play a critical role in the regulation of synapse formation and neuronal specification. The cellular and molecular mechanisms by which neurons and astrocytes communicate and coordinate are not well understood. The experiments in this proposal will uncover the molecular and cellular mechanisms by which neuron derived SHH protein can coordinate the cell behaviors of cortical astrocytes to facilitate the formation and function of neural circuits in the brain. In our studies we take advantage of the large complement of mouse genetic tools that allows us to specifically manipulate Shh signaling in astrocytes. Using cutting edge light and electron microscopy approaches we will define how specific interactions between astrocytes and neurons are dependent on Shh signaling and assess how astrocytes influence the development of functional circuits. Collectively these aims will provide a framework for understanding the delivery, reception and cellular response to SHH in the developing cortex.