权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Polycystins and Cilia in C. elegans

线虫中的多囊蛋白和纤毛

基本信息

批准号：
8465215
负责人：
MAUREEN M BARR
金额：
$ 31.29万
依托单位：
RUTGERS, THE STATE UNIV OF N.J.
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-01 至 2015-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8465215
关键词：
1-Phosphatidylinositol 4-Kinase Address Affect Afferent Neurons Animals Autosomal Dominant Polycystic Kidney Behavior Biochemical Biological Biological Models Biology Caenorhabditis elegans Candidate Disease Gene Cells Cellular biology Chromosome Mapping Cilia Complex Cystic kidney Defect Development Diffusion Disease Double-Stranded RNA Down-Regulation Electrophysiology (science)End stage renal failure Enzymes Essential Genes Esthesia Excision Genes Genetic Goals Health Hereditary Disease Homologous Gene Human Human Development Human Genetics Image Individual Ion Channel Joubert syndrome Kidney Diseases Laboratories Length Mammalian Cell Mechanics Medical Modeling Molecular Molecular Genetics Molecular Motors Morphogenesis Motor Mus Mutate Mutation Nematoda Neurons Organ Organelles PKD2 protein Partner in relationship Phosphatidylinositols Phosphoric Monoester Hydrolases Play Post-Translational Protein Processing Process Proteins Purkinje Cells RNA Interference Reagent Renal function Reproducibility Research Role Sensory Shapes Signal Pathway Signal Transduction Stimulus System Techniques Testing Transgenic Animals Tubulin Tumor Necrosis Factor Receptor Tumor Necrosis Factor-alpha Ubiquitination Work ciliopathy cilium biogenesis feeding human TNF protein human disease in vivo insight interdisciplinary approach male molecular imaging novel novel strategies polycystic kidney disease 1 protein promoter protein function receptor sensor tool trafficking ubiquitin-protein ligase

项目摘要

DESCRIPTION (provided by applicant): Cilia are cellular organelles that are important for human development, organ function, and sensation. Defects in cilia formation or function results in devastating human diseases or ciliopathies, including autosomal dominant polycystic kidney disease (ADPKD). ADPKD affects 1/400-1000 individuals, often resulting in end- stage renal disease. In humans, mutations in the polycystin-1 or polycystin-2 receptor-channel complex cause ADPKD. Despite the profound medical importance of cilia in human health, how cilia are specialized in form and function remains poorly understood. This proposed research is focused on uncovering fundamental principals of cilia biology not possible in human studies or easily studied in vertebrate model systems. C. elegans is a transparent, multicellular animal with specialized sensory cilia, features that enable in vivo imaging that is unprecedented in its simplicity and reproducibility. We have developed the tools and reagents to study polycystin localization and function in C. elegans. We will employ genetic, molecular, imaging, biochemical, and electrophysiological approaches to address three specific aims. First, we will dissect polycystin signaling pathways. Next, we will determine the molecular details of mechanisms regulating polycystin localization, focusing on the roles of phosphoinositides and tubulin post-translational modifications. Finally, we will identify new genes regulating PKD-2 localization using transgenic animals that are hypersensitive to neuronal RNAi. This multidisciplinary approach will provide a comprehensive picture of the molecules that influence polycystin channel assembly and trafficking, and will provide critical insight to ciliary receptor trafficking in general. Thus these studies will have important implications in human diseases such as ADPKD in which cilia play an important and central role.

描述（由申请人提供）：纤毛是对人类发育、器官功能和感觉非常重要的细胞器。纤毛形成或功能的缺陷导致毁灭性的人类疾病或纤毛病，包括常染色体显性遗传性多囊肾病（ADPKD）。ADPKD影响1/400-1000个体，通常导致终末期肾病。在人类中，多囊蛋白-1或多囊蛋白-2受体通道复合物的突变导致ADPKD。尽管纤毛在人类健康中具有深刻的医学重要性，但纤毛如何在形式和功能上特化仍然知之甚少。这项研究的重点是揭示纤毛生物学的基本原理，这在人类研究中是不可能的，在脊椎动物模型系统中也很容易研究。C.线虫是一种透明的多细胞动物，具有专门的感觉纤毛，这些特征使得能够在体内成像，这在其简单性和可重复性方面是前所未有的。我们已经开发了研究多囊蛋白在C.优雅的我们将采用遗传，分子，成像，生物化学和电生理学的方法来解决三个具体的目标。首先，我们将剖析多囊蛋白信号通路。接下来，我们将确定调节多囊蛋白定位机制的分子细节，重点是磷酸肌醇和微管蛋白翻译后修饰的作用。最后，我们将使用对神经元RNAi过敏的转基因动物来鉴定调节PKD-2定位的新基因。这种多学科的方法将提供一个全面的图片的分子，影响多囊蛋白通道组装和贩运，并将提供关键的洞察力纤毛受体贩运一般。因此，这些研究将对人类疾病如ADPKD具有重要意义，其中纤毛发挥重要和核心作用。