Molecular Biology

分子生物学

• 批准号: 8435432
• 负责人: Takatoshi Mochizuki
• 金额: $ 19.42万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8435432
• 关键词: Arousal; Complex; DNA; Ensure; Genotype; Histocytochemistry; In Situ Hybridization; Laboratories; Methods; Molecular; Molecular Biology; Mus; Performance; Plasmids; Research Personnel; Science; Services; Sleep; Sleep Apnea Syndromes; Techniques; Technology; Viral Vector; design; experience; neuromechanism; success

The Molecular Biology Core will provide expert and efficient laboratory services to Project 1 (Saper), Project 3 (Scammell), and Project 5 (Chamberiin). The major duties of Core B are management and genotyping of mouse colonies and performing in situ hybridization histochemistry. Core B will also produce new bacterial clones for in situ hybridization and new viral vectors as technology evolves. Core B is designed to optimize efficiency, accuracy, and expertise. The Core will manage large, shared mouse colonies to ensure a reliable and efficient supply of accurately genotyped mice for each Project. The Core also will centralize the performance of complex molecular techniques such as in situ hybridization histochemistry and bacterial culture so researchers in the Projects can focus on the science instead of debugging techniques Our years of experience with these methods maximizes the likelihood that the complicated molecular techniques proposed in this PPG will succeed.

分子生物学核心将为项目1（Saper），项目3（Scammell）和项目5（Chamberiin）提供专业和高效的实验室服务。核心B的主要职责是管理和小鼠集落的基因分型，并进行原位杂交组织化学。随着技术的发展，核心B还将产生用于原位杂交的新细菌克隆和新的病毒载体。 Core B旨在优化效率、准确性和专业知识。核心将管理大型共享小鼠群，以确保为每个项目提供可靠有效的准确基因分型小鼠。核心还将集中复杂的分子技术，如原位杂交组织化学和细菌培养的性能，使研究人员在项目中可以专注于科学，而不是调试技术，我们多年的经验，这些方法最大限度地提高了可能性，复杂的分子技术提出了这个PPG将成功。