Core B _BIDMC

酷睿B概述 分子生物学核心(核心B)将提供专业和高效的实验室服务 项目1(Saper)、项目2(Scammell)和项目4(Chamberlin)。主要职责 核心B是对小鼠群体的管理和基因分型，并在现场进行 杂交组织化学。核心B还将为就地管理细菌克隆 杂交和病毒载体，并生产新的/修饰的DNA结构作为技术 不断进化。 核心B旨在优化疗效、准确性和专业知识。核心将管理 大型共享小鼠群体，确保可靠、高效的精准供应 每个项目的小鼠基因分型。核心还将集中执行 原位杂交、组织化学和细菌等复杂分子技术 文化，这样项目中的研究人员就可以专注于科学而不是调试 技巧。我们多年使用这些方法的经验最大限度地增加了 在这个PPG中提出的复杂的分子技术将会成功。

Summary of Core B The Molecular Biology Core (Core B) will provide expert and efficient laboratory services to Project 1 (Saper), Project 2 (Scammell) and Project 4 (Chamberlin). The major duties of Core B are management and genotyping of mouse colonies and performing in situ hybridization histochemistry. Core B will also manage bacterial clones for in situ hybridization and viral vectors, and produce new/modified DNA construct as technology evolves. Core B is designed to optimize efficacy, accuracy and expertise. The Core will manage large, shared mouse colonies to ensure a reliable and efficient supply of accurately genotyped mice for each Project. The Core also will centralize the performance of complex molecular techniques such as in situ hybridization histochemistry and bacterial culture so researchers in the Projects can focus on the science instead of debugging techniques. Our years of experience with these methods maximizes the likelihood that the complicated molecular techniques proposed in this PPG will succeed.