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Core B _BIDMC

核心B_BIDMC

基本信息

批准号：
8794519
负责人：
Takatoshi Mochizuki
金额：
$ 22.1万
依托单位：
BETH ISRAEL DEACONESS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-03-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8794519
关键词：
Antisense RNA Arousal Autoradiography Bacteriophage P1 Brain region Breeding Budgets Choline O-Acetyltransferase Complex DNA Digoxigenin Emulsions Engineering Ensure Film Funding Future Genes Genetic Recombination Genotype Glutamate Transporter Goals Histocytochemistry Hypercapnia In Situ Hybridization Label Laboratories Maintenance Messenger RNA Methods Microinjections Microscopy Mission Molecular Molecular Biology Mus Mutant Strains Mice Neurons Pattern Performance Phenotype Plasmids Preparation Production Progress Reports Protocols documentation Publications RNA Probes Reagent Recombinants Research Personnel Scheme Science Series Services Signal Transduction Sleep Apnea Syndromes Techniques Technology Tissues Type I DNA Topoisomerases Viral Viral Vector Work adeno-associated viral vector base brain tissue cost design experience improved mRNA Expression mutant mouse model neurochemistry neuromechanism novel promoter recombinase response skills success tool vesicular GABA transporter

项目摘要

Summary of Core B The Molecular Biology Core (Core B) will provide expert and efficient laboratory services to Project 1 (Saper), Project 2 (Scammell) and Project 4 (Chamberlin). The major duties of Core B are management and genotyping of mouse colonies and performing in situ hybridization histochemistry. Core B will also manage bacterial clones for in situ hybridization and viral vectors, and produce new/modified DNA construct as technology evolves. Core B is designed to optimize efficacy, accuracy and expertise. The Core will manage large, shared mouse colonies to ensure a reliable and efficient supply of accurately genotyped mice for each Project. The Core also will centralize the performance of complex molecular techniques such as in situ hybridization histochemistry and bacterial culture so researchers in the Projects can focus on the science instead of debugging techniques. Our years of experience with these methods maximizes the likelihood that the complicated molecular techniques proposed in this PPG will succeed.

酷睿B概述分子生物学核心(核心B)将提供专业和高效的实验室服务项目1(Saper)、项目2(Scammell)和项目4(Chamberlin)。主要职责核心B是对小鼠群体的管理和基因分型，并在现场进行杂交组织化学。核心B还将为就地管理细菌克隆杂交和病毒载体，并生产新的/修饰的DNA结构作为技术不断进化。核心B旨在优化疗效、准确性和专业知识。核心将管理大型共享小鼠群体，确保可靠、高效的精准供应每个项目的小鼠基因分型。核心还将集中执行原位杂交、组织化学和细菌等复杂分子技术文化，这样项目中的研究人员就可以专注于科学而不是调试技巧。我们多年使用这些方法的经验最大限度地增加了在这个PPG中提出的复杂的分子技术将会成功。