THz imaging of acute burn wounds

急性烧伤创面太赫兹成像

• 批准号: 8512614
• 负责人: WARREN S GRUNDFEST
• 金额: $ 21.2万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-04-10 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8512614
• 关键词: Acute; Animals; Area; Blood; Burn Centers; Burn injury; Calibration; Clinical; Coagulation Process; Companions; Data; Diagnosis; Dyes; Edema; Eye; Feedback; Goals; Hematoxylin and Eosin Staining Method; Hour; Hydration status; Image; Imagery; Imaging Device; Injury; Lasers; Lighting; Literature; Maps; Measurement; Measures; Methods; Modeling; Pathologic; Pathology; Photons; Rattus; Resolution; Scanning; Secondary to; Severities; Staining method; Stains; Surgeon; System; Tactile; Techniques; Thick; Tissues; Visual; Water; Work; absorption; base; blood perfusion; diagnostic accuracy; experience; heat injury; in vivo; prevent; public health relevance; research study; response; tool; wound

DESCRIPTION (provided by applicant): The goal of this proposal is to develop a tissue hydration imaging system based on THz and NIR illumination to spatially and temporally map the hydration state of burn wounds and correlate these measurements with burn severity. Burn severity is typically assessed with visual and tactile feedback from the surgeon and diagnostic accuracy varies widely based on the clinical presentation and the surgeon's experience. In a large number of burn centers complementary assessments are made by burn wound blood perfusion measured with Laser Doppler Imaging (LDI) and vital dye based systems. These techniques are based on blood perfusion and do not provide immediate assessment of burn wound severity due to the evolving response to the burn injury. This limitation prevents the use of these techniques during the first 48 hours following injury leading to a delay in optimal management. Preliminary in vivo results obtained by our group in rats suggest that hydration imaging acquired with THz and NIR illumination may provide information to non-invasively assess burn severity within the first few hours of injury. This information may allow the burn surgeon to rapidly differentiate between partial and full thickness injury. THz and NIR illumination are eye safe, have very low photon energy, and are extremely sensitive to hydration changes secondary to edema which is part of the tissue response to thermal injury. This study will begin with the construction of a scanned beam, THz imaging system capable of non-contact, imaging of a 10 cm x 10 cm square area in less than 30 seconds. A companion NIR imaging system tuned to the peak absorption wavelength of water will be developed and used for comparison. We will also provide additional information regarding wound hydration. Year 2 will encompass extensive animal work in rats (N = 20) and the capability of these imaging systems for burn severity diagnosis will be evaluated against clinical and histopathological diagnosis.

描述（由申请人提供）：本提案的目标是开发一种基于THz和NIR照明的组织水合成像系统，以在空间和时间上映射烧伤伤口的水合状态，并将这些测量结果与烧伤严重程度相关联。烧伤严重程度通常通过外科医生的视觉和触觉反馈进行评估，诊断准确性根据临床表现和外科医生的经验而变化很大。在大量的烧伤中心，通过激光多普勒成像（LDI）和基于活体染料的系统测量烧伤伤口血液灌注来进行补充评估。这些技术基于血液灌注，并且由于对烧伤的反应不断变化而不能提供对烧伤伤口严重程度的即时评估。这种局限性阻止了在受伤后的前48小时内使用这些技术，导致最佳管理的延迟。我们的小组在大鼠中获得的初步体内结果表明，用THz和NIR照明获得的水合成像可以提供信息，以非侵入性地评估烧伤的严重程度在受伤的最初几个小时内。该信息可允许烧伤外科医生快速区分部分厚度损伤和全厚度损伤。THz和NIR照明对眼睛是安全的，具有非常低的光子能量，并且对继发于水肿的水合变化非常敏感，水肿是组织对热损伤的反应的一部分。这项研究将开始与扫描光束，太赫兹成像系统的建设能够非接触，成像的10厘米× 10厘米平方米的面积在不到30秒。一个配套的近红外成像系统调谐到水的峰值吸收波长将被开发和用于比较。我们还将提供有关伤口水化的其他信息。第2年将包括在大鼠（N = 20）中进行广泛的动物研究，并将根据临床和组织病理学诊断评价这些成像系统用于烧伤严重程度诊断的能力。