A Correlational Study of THz and MRI Hydration Mapping in In vivo Skin Burns

体内皮肤烧伤中太赫兹和 MRI 水合图的相关性研究

• 批准号: 8912471
• 负责人: WARREN S GRUNDFEST
• 金额: $ 22.3万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-15 至 2017-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8912471
• 关键词: Acute; Animals; Area; Behavior; Burn injury; Care Technology Points; Clinical; Confidence Intervals; Data; Diagnosis; Diagnostic; Early Diagnosis; Functional disorder; Health; Hematoxylin and Eosin Staining Method; Histology; Hour; Hydration status; Image; Imagery; Imaging Techniques; Inflammatory Response; Injury; Lasers; Left; Life; Liquid substance; Logistics; Magnetic Resonance Imaging; Maps; Measurement; Measures; Medical; Modality; Modeling; Monitor; Neighborhoods; Optics; Pathology; Phase; Physiological; Polyhydroxyethyl Methacrylate; Procedures; Property; Rattus; Relaxation; Research; Sample Size; Sensitivity and Specificity; Severities; Skin; Slice; Specificity; Staining method; Stains; System; Tactile; Testing; Thick; Time; Tissues; Visual; Water; Water Movements; Weight; Work; accurate diagnosis; base; blood perfusion; image registration; imaging system; in vivo; in vivo Model; indexing; injured; insight; non-invasive imaging; novel; outcome forecast; rapid detection; research study; response; tool; treatment center; trend; wound

DESCRIPTION (provided by applicant): This proposal aims to compare terahertz (THz) reflectivity maps and MRI hydration indicators, specifically changes in T2 relaxation times, to validate tissue water content as the dominant contrast mechanism in THz burn imaging and study differences in THz imagery of partial and full thickness burn pathophysiology for early and accurate diagnosis of wound severity. Burn extent is most often examined by visual and tactile assessment with successful prognosis in only 50-70% of cases. Several burn treatment centers have implemented Laser Doppler Imaging (LDI) as a complementary diagnostic; however, this procedure relies on measurements of blood perfusion which can be a poor marker immediately following thermal insult. Preliminary in vivo results acquired by our group in rats suggest that ou non-invasive THz imaging system rapidly and accurately generates skin reflectivity imagery of burns; clearly delineating burn wound zones and burn severity within the first few hours of injury. Resultant changes in the optical properties of tissue by burn induction create contrast between injured and uninjured areas evident in acquired reflective THz images. However, THz reflectivity values around the burn region may often be spatially and temporally variable, and these findings do not always correlate with histological analysis. Given its advantages in tissue specificity and water sensitivity, MRI can be used as a supplementary tool to further understand and substantiate what appear to be hydration changes in THz maps of burn pathophysiology and reinforce the clinical potential of medical THz imaging as a point-of-care technology for rapid detection of burn wounds. Recent T2 weighted multi slice multi echo (T2w MSME) 7 Tesla MR and THz parallel imaging studies in in vivo rat skin demonstrate that both modalities can be used to localize the burn region and detect water movement in tissue; T2 relaxation times, a common MR index of hydration, and THz reflectivity measurements of the burn area increase following burn induction. These preliminary results appear to support the sufficiency of the sensitivity and specificity of MRI for measuring tissue water content and the use of this modality to verify the hydration sensing capabilities of THz burn imaging for acute diagnosis of skin burns and monitoring of edematous responses. This study will commence with pilot, parallel MRI and THz in vivo skin imaging experiments, providing the necessary groundwork for large scale in vivo rat studies. Year 2 will encompass extensive work on 20 live rats, a sample size based on a paired t-test with a power of 0.9 and a confidence interval of 0.05. Correlations between these modalities will be assessed by co-registration with histological analysis.

描述（由申请人提供）：本提案旨在比较太赫兹（THz）反射率图和MRI水合指标，特别是T2弛豫时间的变化，以验证组织含水量作为THz烧伤成像的主要对比机制，并研究部分和全层烧伤病理生理学THz成像的差异，以早期准确诊断伤口严重程度。烧伤程度通常通过视觉和触觉评估进行检查，只有50-70%的病例预后良好。几个烧伤治疗中心已实施激光多普勒成像（LDI）作为补充诊断;然而，该程序依赖于血液灌注的测量，这可能是热损伤后立即出现的不良标记。我们的小组在大鼠中获得的初步体内结果表明，非侵入性THz成像系统快速准确地生成烧伤的皮肤反射率图像;在受伤的最初几个小时内清楚地描绘烧伤伤口区域和烧伤严重程度。 烧伤诱导导致的组织光学性质的变化在所获得的反射THz图像中明显地在受伤区域和未受伤区域之间产生对比。然而，烧伤区域周围的THz反射率值可能经常是空间和时间可变的，并且这些发现并不总是与组织学分析相关。鉴于其在组织特异性和水敏感性方面的优势，MRI可用作补充工具，以进一步了解和证实烧伤病理生理学THz图中的水合变化，并加强医学THz成像作为快速检测烧伤伤口的即时技术的临床潜力。最近的T2加权多切片多回波（T2 W MSME）7特斯拉MR和THz并行成像研究在体内大鼠皮肤表明，这两种模式可用于定位烧伤区域和检测组织中的水运动; T2弛豫时间，一个共同的MR水合指数，和THz反射率测量烧伤面积增加烧伤诱导。这些初步结果似乎支持足够的灵敏度和特异性的MRI测量组织含水量和使用这种方式来验证太赫兹烧伤成像的水化传感能力的皮肤烧伤的急性诊断和监测水肿反应。本研究将开始试点，并行MRI和太赫兹在体内皮肤成像实验，提供必要的基础，大规模在体内大鼠研究。第2年将包括对20只活大鼠的广泛研究，样本量基于配对t检验，功效为0.9，置信区间为0.05。将通过与组织学分析的共配准来评估这些模式之间的相关性。