Supramolecular Nanoparticle-Based PET Probes for Pretargeted Tumor Imaging

用于预定位肿瘤成像的超分子纳米颗粒 PET 探针

• 批准号: 8425970
• 负责人: HSIAN-RONG TSENG
• 金额: $ 19.25万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-12-15 至 2014-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8425970
• 关键词: Adopted; Affect; Affinity; Animals; Biological; Cancer Biology; Categories; Characteristics; Chemistry; Collection; Coupling; Data; Diagnostic Imaging; Drug Delivery Systems; Drug Kinetics; Encapsulated; Exhibits; Future; Goals; Histology; Image; Injection of therapeutic agent; Joints; Kinetics; Label; Length; Ligands; Ligation; Liver; Molecular; Motivation; Organ; Outcome; Performance; Permeability; Polymers; Positron; Positron-Emission Tomography; Radiochemistry; Radioisotopes; Radiolabeled; Reaction; Reagent; Reporter; Research; Research Personnel; Solid Neoplasm; Surface Properties; Time; Xenograft procedure; base; controlled release; design; dosage; imaging probe; improved; in vivo; molecular assembly/self assembly; molecular imaging; mouse model; nanoparticle; novel strategies; pre-clinical; public health relevance; radiotracer; small molecule; tumor; uptake; vector

DESCRIPTION (provided by applicant): The long-term objective of this R21 proposal is to develop a new class of nanoparticle (NP)-based positron emission tomography (PET) probes that enable high-performance tumor imaging. We propose to adopt a pretargeted imaging strategy to decouple the NP components from their corresponding radiolabeled reporters. First, a pair of tumor-targeting NP component and radiolabeled reporter with desired PKs will be synthesized separately via rational molecular designs. We will then modulate the interplay between other experimental variables such as injection times and dosage in order to achieve optimal PET imaging outcomes. A prerequisite to successful pretargeted imaging is to accomplish selective and irreversible coupling of the tumor- targeting NP and sequentially injected radiolabeled reporter in vivo. We thus exploit the use of a bioorthogonal conjugation chemistry based on a pair of reactive motifs, i.e., trans-cyclooctene (TCO) and tetrazine (Tz), which have fast reaction kinetics and biological stability. Future progress in PET imaging will involve designing molecular imaging probes that preferentially accumulate in tumors. Aside from small molecule and affinity ligand-based PET imaging probes, NPs exhibiting unique enhanced permeability and retention (EPR) effects represent a new category of PET probes capable of passively targeting leaky vasculature - a universal characteristic observed for most solid tumors. While a variety of NP PET probes have been examined in pre-clinical setting, challenges remain to further improve tumor uptake and reduce nonspecific distribution in other organs. In our molecular design, the TCO motif is covalently attached onto a polymer building block of supra-molecular nanoparticle (SNP). Self-assembly of the molecular building blocks leads to encapsulation of TCO to yield TCO-encapsulated SNP (TCO?SNP) as the tumor-targeting NP component. Further, the radiolabeled reporter is composed of the complementary Tz motif and 18F-tag. In the proposed PET imaging study, TCO?SNP is first administered to an animal. When the TCO?SNPs approach their optimal accumulation in tumor, the radiolabeled reporter is then injected. In vivo bio-orthogonal reaction occurs instantaneously, resulting in high-contras PET imaging. Our joint team has some preliminary data supporting the feasibility of this new class of NP PET imaging probes. We will implement the following two Specific Aims to accomplish our research endeavors, 1) Prepare and select TCO?SNPs and radiolabeled reporters with optimal PKs, and 2) In vivo demonstration of pretargeted PET imaging using pairs of TCO?SNPs and radiolabeled reporters. This proposal brings together the expertise of four research groups (PI and 3 co-investigators) covering the fields of supramolecular chemistry, nanoparticle, radiochemistry, molecular imaging and cancer biology. We envision that the successful demonstration of our proposed research could change current paradigm in oncologic PET imaging, and open up new opportunities for pretargeted drug delivery.

描述（由申请人提供）：该R21提案的长期目标是开发一类新的基于纳米颗粒（NP）的正电子发射断层扫描（PET）探针，使高性能肿瘤成像成为可能。我们建议采用一种预先定位的成像策略，将NP成分与其相应的放射性标记报告分离。首先，通过合理的分子设计，分别合成一对肿瘤靶向NP组分和具有所需PKs的放射性标记报告蛋白。然后，我们将调节其他实验变量（如注射时间和剂量）之间的相互作用，以获得最佳的PET成像结果。成功的预靶向成像的先决条件是实现肿瘤靶向NP与体内顺序注射的放射性标记报告基因的选择性和不可逆偶联。因此，我们利用基于一对反应基序的生物正交偶联化学，即反式环烯（TCO）和四嗪（Tz），它们具有快速的反应动力学和生物稳定性。PET成像的未来进展将包括设计优先在肿瘤中积累的分子成像探针。除了基于小分子和亲和配体的PET成像探针外，具有独特的增强渗透和保留（EPR）效应的NPs代表了一种新型PET探针，能够被动靶向泄漏的血管系统-这是大多数实体肿瘤观察到的普遍特征。虽然各种NP PET探针已经在临床前环境中进行了研究，但进一步提高肿瘤摄取和减少非特异性分布在其他器官中的挑战仍然存在。在我们的分子设计中，TCO基序共价连接到超分子纳米颗粒（SNP）的聚合物构建块上。分子构建块的自组装导致TCO的封装，从而产生TCO封装的SNP (TCO？SNP)作为肿瘤靶向NP成分。此外，放射性标记的报告基因由互补的Tz基序和18f标签组成。在提议的PET成像研究中，TCO？SNP首先用于动物。TCO是多少？snp接近其在肿瘤中的最佳积累，然后注射放射性标记的报告基因。体内生物正交反应瞬间发生，导致PET成像高对比度。我们的联合团队有一些初步数据支持这种新型NP PET成像探针的可行性。我们将实施以下两个具体目标来完成我们的研究工作：1)准备和选择TCO？SNPs和具有最佳PKs的放射性标记报告基因，以及2)使用对TCO？snp和无线电标记的报告。该提案汇集了四个研究小组（PI和3名合作研究人员）的专业知识，涵盖超分子化学，纳米粒子，放射化学，分子成像和癌症生物学领域。我们设想，我们提出的研究的成功演示可能会改变目前肿瘤PET成像的范式，并为预靶向药物递送开辟新的机会。