Multiplexed, Rapid, Point of Care Device to Quantify Specific IgE to Common Aller

描述(由申请人提供)：拟议研究的主要目标是开发一种校准的、紧凑的、廉价的、多路复用的、具有结合荧光和无标签能力的适用于医疗诊断领域的护理点传感平台。使用体外记录的过敏原特异性IgE患者的血清和临床证据作为模型系统，我们将开发一个护理点平台，将我们成熟的无标记传感技术与我们的增强型荧光技术-校准荧光增强(CAFE)相结合。我们认为，该平台将更准确和灵敏地检测到针对感兴趣蛋白质的特定抗体的存在。护理点能力将使技术得到有效应用，并加强患者护理。当前测试的局限性在于使用远离护理点的临床或参考实验室中的荧光测量，而捕获感兴趣的蛋白质(例如抗体)的探针尚未校准，并且因芯片而异。此外，目前可用的定量无标签传感器仅限于灵敏度水平超过设备的噪声下限。这项工作将设计一种校准仪器，它结合了双重模式，以改进现有的诊断技术。虽然使用过敏诊断作为模型，但基础技术是模块化的，可以应用于肝病和自身免疫性疾病的免疫诊断。

DESCRIPTION (provided by applicant): The major goal of the proposed research is to develop a calibrated, compact, inexpensive, multiplexed, point- of-care sensing platform with combined fluorescence and label-free capabilities applicable to the field of medical diagnostics. Using sera from individuals with documented in vitro and clinical evidence of allergen- specific IgE as a model system, we will develop a point-of-care platform that combines our established label- free sensing technology with our enhanced fluorescence technology, Calibrated Fluorescence Enhancement (CaFE). We propose that this platform will more accurately and sensitively detect the presence of specific antibodies to proteins of interest. The point-of-care capability will allow efficient technology application and enhance patient care. Limitations of current tests reside in utilizing fluorescence measurements in a clinical or reference laboratory away from the point-of-care, while the probes capturing the proteins (e.g. antibodies) of interest have not been calibrated and vary from chip to chip. In addition, currently available quantitative label-free sensors are limited to sensitivity levels surpassing the noise floor of the device. This work will engineer a calibrated instrument, which incorporates dual modalities to improve upon existing diagnostic technology. While using allergy diagnostics as a model, the underlying technology is modular, and could be applied to immunodiagnostics of liver disease and autoimmune disease.