Light Microscopy Core

光学显微镜核心

• 批准号: 8746877
• 负责人: Julie G Donaldson
• 金额: $ 108.2万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8746877
• 关键词: Cellular biology; Collaborations; Color; Comb animal structure; Core Facility; Custom; Data Analyses; Development; Disease; Equipment; Fluorescence; Fluorochrome; Goals; Home environment; Housing; Human Resources; Image; Imaging Techniques; Institutes; Life; Location; Methodology; Microscope; Microscopy; Mission; National Heart, Lung, and Blood Institute; Paper; Publishing; Reporting; Research; Research Personnel; Resolution; Services; Side; Slide; Techniques; Time; Tissues; Training; United States National Institutes of Health; Work; Writing; cellular imaging; digital; experience; flexibility; image processing; instrument; light microscopy; meetings; multi-photon; operation; programs; reconstruction; research study; response; two-photon

The NHLBI Light Microscopy Core Facility (LMF) facility has been in operation for thirteen years. The NHLBI-LMF consists of three people, Dr. Christian A. Combs (Director), Dr. Daniela A. Malide, and Dr. Xufeng Wu (Deputy Director). The facility consists of twenty microscopes located in several locations in buildings 10 and 50 on the main NIH campus in Bethesda, MD. To date we have helped researchers publish more than 190 papers and assisted almost every NHLBI-DIR Center and Branch in the past thirteen years. In 2011-2012 we have help to publish twenty two papers (at the time of this report). Research conducted in the LMF has been on many disease states, basic cell biology, and on development and implementation of new imaging techniques. The general makeup of the NHLBI-LMF reflects the evolving light microscopy needs of NHLBI-DIR researchers and a commitment to provide a maximal level of assistance to the NHLBI mission. The mission of this facility is to provide state-of-the-art equipment, training, and image processing capabilities to assist NHLBI-DIR researchers in experiments involving light microscopy. Researchers that work in this facility can expect support from core personnel to whatever level suits their research. This can include advanced microscopy techniques like intra-vital two-photon microscopy and super-resolution microscopy to more ordinary wide-field imaging with a color camera Our emphasis is on training users to conduct the experiments themselves, although we are available for collaboration and all manner of assistance (experimental planning, data analysis and image processing, etc.) where required. Over the thirteen years this facility has been in existence we have endeavored to provide a flexible and easy to use facility that meets researchers needs and allows them to conduct their experiments in an efficient manner even if they have had no prior microscopy experience. These goals are met in several ways. First, we have an array of microscopes that offer a wide-range of microscopy techniques. These microscopes and the microscopy techniques available have been chosen and developed in response to the specific needs of researchers in the institute. In addition to the microscopes, we provide a full suite of image processing programs in several image processing workstations. Where image-processing capabilities are lacking in these programs we either write our own image processing programs. In summary this facility operates with state of the art equipment and a dedicated team of imaging professionals to further the research mission of the NHLBI-DIR.

NHLBI光学显微镜核心设施（LMF）设施已经运行了13年。 NHLBI-LMF由三个人组成，Christian A. Combs（Director），Daniela A. Malide和Xufeng Wu博士（副主任）。 该设施由20台显微镜组成，位于马里兰州贝塞斯达NIH主校区10号和50号楼的几个位置。 到目前为止，我们已经帮助研究人员发表了190多篇论文，并在过去的十三年中帮助了几乎所有的NHLBI中心和分支。在2011-2012年，我们帮助发表了22篇论文（在本报告撰写时）。 在LMF进行的研究一直在许多疾病状态，基础细胞生物学，以及新的成像技术的开发和实施。 NHLBI-LMF的总体构成反映了NHLBI-LMF研究人员不断发展的光学显微镜需求，并承诺为NHLBI使命提供最大程度的援助。 该设施的使命是提供最先进的设备，培训和图像处理能力，以协助NHLBI-生物医学研究人员在实验中涉及光学显微镜。在该设施工作的研究人员可以期望得到核心人员的支持，无论他们的研究水平如何。 这可以包括先进的显微镜技术，如活体双光子显微镜和超分辨率显微镜，以更普通的宽视场成像与彩色相机我们的重点是培训用户进行实验本身，虽然我们可以合作和各种方式的援助（实验计划，数据分析和图像处理等）。在必要时。 在过去的13年里，该设施一直存在，我们致力于提供一个灵活和易于使用的设施，满足研究人员的需求，并允许他们以有效的方式进行实验，即使他们没有以前的显微镜经验。 这些目标可以通过几种方式实现。 首先，我们有一系列显微镜，提供广泛的显微镜技术。 这些显微镜和显微镜技术是根据研究所研究人员的具体需求选择和开发的。 除了显微镜，我们还在多个图像处理工作站中提供全套图像处理程序。 在这些程序中缺少图像处理能力的地方，我们可以编写自己的图像处理程序。 总之，该设施使用最先进的设备和一个专门的成像专业人员团队，以进一步推进NHLBI的研究使命。