Arf GTP-binding proteins and membrane traffic

Arf6 GTP结合蛋白已被证明在质膜上发挥作用，以协调内体膜运输途径，并维持皮质肌动蛋白细胞骨架。我们继续关注Arf6以及其他Arf蛋白在细胞表面和内膜上的功能。我们还开始研究ARF在其他细胞类型中的功能，特别是T细胞。 我们仍然对Arf蛋白与Rab蛋白协调功能的问题感兴趣。特别是，Arf6和Rab35在招募彼此的差距方面是相互对立的。因此，Arf6的激活导致Rab35的激活减少，而激活的Rab35使Arf6的激活减少。该小组利用这一规定在内体贩运期间促进货物的移交。

The Arf6 GTP-binding protein has been shown to function at the plasma membrane to coordinate an endosomal membrane trafficking pathway and maintain the cortical actin cytoskeleton. We continue to follow functions for Arf6 as well as other Arf proteins at the cell surface and on endosomal membranes. We have also begun to examine functions of Arfs in other cells types in particular T cells. We continue to be interested in the issue of Arf proteins coordinating function with Rab proteins. In particular that Arf6 and Rab35 are antagonistic to each other in recruiting each other's GAPs. Thus activation of Arf6 leads to less activation of Rab35 and active Rab35 diminishes Arf6 activation. The cell uses this regulation to facilitate hand-off of cargo during endosomal trafficking.