Arf GTP-binding proteins and membrane traffic

Arf GTP 结合蛋白和膜运输

• 批准号: 9354305
• 负责人: Julie G Donaldson
• 金额: $ 18.39万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9354305
• 关键词: Actins; Cell membrane; Cell surface; Cells; Cytoskeleton; GTP-Binding Proteins; Goals; Hand; Laboratories; Membrane; Membrane Protein Traffic; Organelles; Pathway interactions; Proteins; Recruitment Activity; Regulation; Sorting - Cell Movement; Structure; T-Lymphocyte; Vesicle; cell type; interest; protein function; trafficking

The Arf6 GTP-binding protein has been shown to function at the plasma membrane to coordinate an endosomal membrane trafficking pathway and maintain the cortical actin cytoskeleton. We continue to follow functions for Arf6 as well as other Arf proteins at the cell surface and on endosomal membranes. We have also begun to examine functions of Arfs in other cells types in particular T cells. We continue to be interested in the issue of Arf proteins coordinating function with Rab proteins. In particular that Arf6 and Rab35 are antagonistic to each other in recruiting each other's GAPs. Thus activation of Arf6 leads to less activation of Rab35 and active Rab35 diminishes Arf6 activation. The cell uses this regulation to facilitate hand-off of cargo during endosomal trafficking.

Arf 6 GTP结合蛋白已被证明在质膜上起作用，以协调内体膜运输途径并维持皮质肌动蛋白细胞骨架。我们继续跟踪Arf 6以及其他Arf蛋白在细胞表面和内体膜上的功能。我们还开始研究Arfs在其他细胞类型，特别是T细胞中的功能。 我们继续对Arf蛋白与Rab蛋白的协调功能的问题感兴趣。特别是Arf6和Rab35在募集彼此的GAP方面彼此拮抗。因此，Arf 6的活化导致Rab35的较少活化，并且活性Rab35减少Arf 6活化。细胞利用这种调节来促进内体运输期间货物的传递。

项目成果

ARF family G proteins and their regulators: roles in membrane transport, development and disease.

• DOI: 10.1038/nrm3117
• 发表时间: 2011-06
• 期刊: Nature reviews. Molecular cell biology

Membrane trafficking heats up in Pavia. Golgi Meeting on Membrane Trafficking in Global Cellular Responses.

帕维亚的膜贩运活动升温。

• DOI: 10.1038/embor.2008.248
• 发表时间: 2009
• 期刊: EMBO reports
• 影响因子: 7.7
• 作者: Donaldson,JulieG;McPherson,PeterS
• 通讯作者: McPherson,PeterS

Clathrin-independent endocytosis: a unique platform for cell signaling and PM remodeling.

• DOI: 10.1016/j.cellsig.2008.06.020
• 发表时间: 2009-01
• 期刊: CELLULAR SIGNALLING
• 影响因子: 4.8
• 作者: Donaldson, Julie G.;Porat-Shliom, Natalie;Cohen, Lee Ann
• 通讯作者: Cohen, Lee Ann

Rab and Arf G proteins in endosomal trafficking and cell surface homeostasis.

Rab 和 Arf G 蛋白在内体运输和细胞表面稳态中的作用。

• DOI: 10.1080/21541248.2016.1212687
• 发表时间: 2016
• 期刊: Small GTPases
• 作者: Donaldson,JulieG;Johnson,DebraL;Dutta,Dipannita
• 通讯作者: Dutta,Dipannita

Phospholipase D in endocytosis and endosomal recycling pathways.

• DOI: 10.1016/j.bbalip.2009.05.011
• 发表时间: 2009-09
• 期刊: Biochimica et biophysica acta
• 作者: Donaldson JG