Reprogramming using small molecules

描述(申请人提供)：四种转录因子(Oct4、Sox2、cMyc和Klf4)的联合活性可以将成体细胞重新编程为诱导的多能干细胞(IPS)。这些重新编程的细胞应该会为移植医学、药物发现和人类疾病研究提供无限的基因定制细胞类型。不幸的是，用于提供重编程因子的方法要么引起了人们对所产生的干细胞未来用途的担忧，要么可能与临床相容的干细胞系的工业化生产不兼容。在这里，我们建议确定小分子的作用机制，我们已经证明，通过增加其效率或允许省略一个或多个重编程因子，可以促进重编程过程。我们的最终目标是朝着鉴定一种化学配方迈进，这种化学配方可以单独将成年细胞重新编程为多能状态。这些重新编程的细胞将不受基因操作的影响，将成为生物医学应用的最佳多潜能细胞。具体目标是：目的1)确定新发现的重编程小分子在重编程过程中取代KLF4的机制；目的2)确定新发现的能够取代Oct4的小重编程分子在重编程过程中的作用机制；目的3)确定我们已经确定的重编程分子是否能够协同作用来取代多个重编程转录因子，从而使我们更接近于采用完全化学的方法进行重编程。

DESCRIPTION (provided by applicant): The combined activity of four transcription factors (Oct4, Sox2, cMyc and Klf4) can reprogram adult cells into induced pluripotent stem (iPS) cells. These reprogrammed cells should provide a limitless supply of genetically tailored cell types for transplantation medicine, drug discovery and the study of human disease. Unfortunately, the methods used to deliver reprogramming factors have either raised concerns regarding the future utility of the resulting stem cells, or may not be compatible with industrial scale production of clinically compatible stem cell lines. Here, we propose to determine the mechanisms of action of small molecules that we have shown can facilitate the reprogramming process by either increasing its efficiency or by allowing the omission of one or more reprogramming factors. Our final goal is to move towards the identification of a chemical formulation that can alone reprogram adult cells to a pluripotent state. These reprogrammed cells would be free of genetic manipulation and would be the optimal pluripotent cells for biomedical applications. Specifically the aims are: aim 1) To determine the mechanisms by which newly identified small reprogramming molecules act to replace Klf4 in the reprogramming process; aim 2) To determine the mechanisms by which newly identified small reprogramming molecules that can replace Oct4 act in the reprogramming process; aim 3) To determine whether the reprogramming molecules that we have identified can act synergistically to replace multiple reprogramming transcription factors, thus moving us closer to a completely chemical method for reprogramming.