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Genetic Analysis of Nematode Behavior

线虫行为的遗传分析

基本信息

批准号：
8389585
负责人：
SCOTT W EMMONS
金额：
$ 30.13万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-08-01 至 2014-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8389585
关键词：
Ablation Address Afferent Neurons Animal Behavior Animal Model Animals Architecture Behavior Behavioral Biological Neural Networks Caenorhabditis elegans Cells Characteristics Chemical Synapse Chemicals Communication Complex Connexins Copulation Diagnosis Electrical Synapse Electrons Elements Excitatory Synapse Family Foundations Gap Junctions Genes Genetic Genetic Models Individual Interneurons Invertebrates Mental disorders Nematoda Nervous System Physiology Nervous system structure Neurons Neuropil Neurotransmitter Receptor Neurotransmitters Output Partner in relationship Pathway interactions Pattern Play Process RNA Interference Recurrence Relative (related person)Research Role Sensory Series Synapses System Testing Vulva base cell killing chemical elimination detector feeding genetic analysis inhibitor/antagonist insight killings male mutant nervous system disorder neural circuit neuron component neurotransmission programs public health relevance reconstruction research study sperm cell

项目摘要

DESCRIPTION (provided by applicant): This research seeks to understand how the activities of individual neurons combine together in defined circuits to generate the behavioral output of the nervous system. The behavior to be studied is the copulatory behavior of the nematode Caenorhabditis elegans male. Using reconstruction from serial section electron micrographs, the complete pattern of synaptic connectivity has been determined for the region of the male nervous system that generates copulatory behavior. This neuropil contains the processes of 175 neurons connected by some 8000 synapses (4000 chemical, 4000 electrical). The wiring diagram, which consists of a complex network of interactions, serves as the basis for examining the role of individual neurons in generating behavioral output. C. elegans male copulatory behavior consists of a series of sub-behaviors executed according to sensory input to allow the male to insert hardened spicules into the hermaphrodite vulva and transfer sperm. Neurons have been identified that by their pattern of connectivity appear to be associated with each of these sub-behaviors. We will test our hypothesis for the function of these neurons by eliminating them from the circuit by cell killing. The behavioral deficit that results will indicate the role of the neuron in the circuit. An important question to be addressed is whether the network contains circuits or modules dedicated to specific sub-behaviors or instead have a distributed type of architecture, generating multiple behaviors as alternative modes of function of the whole. To assess the relative importance of chemical and electrical communication within the network, the neurotransmitters utilized will be determined. Chemical neurotransmission will be blocked using mutants or through cell-specific RNAi approaches to reveal the function of the electrical circuit. The wiring diagram contains a repeated network motif in which a sensory neuron and an interneuron are connected both directly and via a pair of interneurons that is common to all, forming a triangular circuit. This motif may have one of several possible influences on the function of the network, depending on whether the common neuron pair is excitatory or inhibitory on the interneuron. In order to determine the functional characteristics of this motif, the neurotransmitter and receptor types in the circuit will be determined and the result of activating or inhibiting the circuit will be determined.

描述（由申请人提供）：本研究旨在了解单个神经元的活动如何在确定的电路中结合在一起，以产生神经系统的行为输出。待研究的行为是秀丽隐杆线虫雄性的交配行为。利用连续切片电子显微图的重建，已经确定了雄性神经系统中产生交配行为的区域的突触连接的完整模式。这个神经系统包含175个神经元的过程，由大约8000个突触连接（4000个化学突触，4000个电突触）。接线图由一个复杂的相互作用网络组成，是研究单个神经元在产生行为输出中的作用的基础。秀丽隐杆线虫的雄性交配行为由一系列根据感官输入执行的子行为组成，这些子行为允许雄性将硬化的针状体插入雌雄同体的外阴并转移精子。神经元的连接模式似乎与这些子行为都有关联。我们将通过杀死细胞将这些神经元从回路中清除，来验证我们对这些神经元功能的假设。行为缺陷的结果将表明神经元在回路中的作用。需要解决的一个重要问题是，该网络是否包含专用于特定子行为的电路或模块，还是具有分布式架构类型，生成多个行为作为整体功能的可选模式。为了评估神经网络中化学和电子通讯的相对重要性，将确定所使用的神经递质。化学神经传递将被阻断使用突变体或通过细胞特异性RNAi方法来揭示电路的功能。接线图包含一个重复的网络主题，其中一个感觉神经元和一个中间神经元直接连接，也通过一对共同的中间神经元连接，形成一个三角形电路。这个基序可能对网络的功能有几种可能的影响之一，这取决于共同神经元对对中间神经元是兴奋性的还是抑制性的。为了确定该基序的功能特征，将确定回路中的神经递质和受体类型，并确定激活或抑制回路的结果。