Regulation of Urea Transport by PKC-alpha

PKC-alpha 对尿素运输的调节

• 批准号: 8735034
• 负责人: JEFF M. SANDS
• 金额: $ 4.94万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8735034
• 关键词: Agonist; Amino Acids; Anatomy; Apical; Calcineurin; Carrier Proteins; Cell membrane; Cells; Consensus; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Cytoplasmic Vesicles; Data; Defect; Development; Diabetes Mellitus; Duct (organ) structure; Equilibrium; Future; Goals; Homeostasis; Isoenzymes; Kidney; Kidney Concentrating Ability; Knockout Mice; Location; Mediating; Membrane; Mutant Strains Mice; Nephrogenic Diabetes Insipidus; Permeability; Phosphoric Monoester Hydrolases; Phosphorylation; Phosphorylation Site; Play; Protein Dephosphorylation; Protein Kinase C; Protein Kinase C Alpha; Protein Kinase C Inhibitor; Protein Phosphatase 2A Regulatory Subunit PR53; Proteins; Rattus; Regulation; Role; Serine; Signal Pathway; Site; Sodium; Stimulus; System; Testing; Urea; Urine; Vasopressins; Water; activator 1 protein; apical membrane; aquaporin-2; base; citrate carrier; novel therapeutic intervention; phosphatase inhibitor; translational study; urea transporter; urinary

DESCRIPTION (provided by applicant): Vasopressin regulates urea transport by activating two cyclic AMP (cAMP) dependent signaling pathways: protein kinase A and exchange protein activated by cAMP. This results in increases of both the phosphorylation and apical plasma membrane accumulation of the UT-A1 urea transporter. The hypertonicity present in the inner medulla can act independently of vasopressin as a powerful stimulus of urea transport. Hypertonicity stimulates urea transport by increasing both UT-A1 phosphorylation and plasma membrane accumulation. However, the signaling pathway by which this occurs is unknown. We have new preliminary data showing that hypertonicity stimulates urea permeability via protein kinase C (PKC). We also have preliminary data suggesting that PKC1 is the specific PKC isozyme involved since: 1) hypertonicity activates PKC1 in rat inner medullary collecting ducts (IMCDs); and 2) PKC1 knock-out mice have a urine concentrating defect and a reduction in UT-A1 protein abundance. Thus, maximal stimulation of urea transport and UT-A1 activity in the terminal IMCD requires stimulation by both cAMP and PKC1. Our overall goal is to investigate the regulation of UT-A1 by PKC. We will test the hypothesis that PKC1 stimulates UT-A1 function through changes in UT-A1 phosphorylation. Aim 1 will determine the mechanism by which PKC regulates UT-A1 phosphorylation. Aim 2 will determine the site(s) in UT-A1 and the cellular location of phosphorylation by PKC. Aim 3 will determine the interdependence of PKC and PKA in the regulation of UT-A1 membrane accumulation, activity, phosphorylation, and phosphatase-mediated dephosphorylation. Aim 4 will determine the mechanism for the urine concentrating defect in PKC" knock-out mice. Our proposed studies are highly significant as they are likely to yield new information on mechanisms underlying dysregulation of water homeostasis. Elucidation of non-cAMP mechanisms for increasing urea transport, which in turn would increase urine concentrating ability, could form the basis for future translational studies of novel therapeutic approaches to congenital nephrogenic diabetes insipidus.

性状（由申请方提供）：加压素通过激活两条环腺苷酸（cAMP）依赖性信号通路（蛋白激酶A和cAMP激活的交换蛋白）调节尿素转运。这导致UT-A1尿素转运蛋白的磷酸化和顶端质膜积累增加。存在于内髓质的高渗性可以独立于加压素作为尿素转运的强有力刺激而起作用。高渗通过增加UT-A1磷酸化和质膜积累来刺激尿素转运。然而，发生这种情况的信号通路是未知的。我们有新的初步数据表明，高渗通过蛋白激酶C（PKC）刺激尿素渗透性。我们也有初步的数据表明，PKC 1是特定的PKC同工酶参与，因为：1）高渗激活PKC 1在大鼠内髓集合管（IMCD）;和2）PKC 1敲除小鼠有尿浓缩缺陷和UT-A1蛋白丰度减少。因此，在终末IMCD中，尿素转运和UT-A1活性的最大刺激需要cAMP和PKC 1的刺激。我们的总体目标是研究PKC对UT-A1的调控。我们将检验PKC 1通过改变UT-A1磷酸化来刺激UT-A1功能的假设。目的1将确定PKC调节UT-A1磷酸化的机制。目的2确定UT-A1的位点和PKC磷酸化的细胞定位。目的3将确定PKC和PKA在调节UT-A1膜积累、活性、磷酸化和磷酸酶介导的去磷酸化中的相互依赖性。目的4探讨蛋白激酶C基因敲除小鼠尿浓缩缺陷的机制。我们提出的研究是非常重要的，因为它们可能会产生新的信息的机制失调的水稳态。阐明增加尿素转运的非cAMP机制，这反过来又会增加尿液浓缩能力，可以为未来先天性肾源性尿崩症新治疗方法的转化研究奠定基础。