Spinal Mechanisms Underlying SCI-Induced Pain: Implications for Targeted Therapy

SCI 引起的疼痛的脊柱机制：对靶向治疗的影响

• 批准号: 8447411
• 负责人: SUSAN G DORSEY
• 金额: $ 67.03万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-21 至 2017-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447411
• 关键词: Address; Affect; American; Americas; Animal Model; Animals; Antibodies; Astrocytes; Behavior; Bilateral; Binding; Biological Assay; Brain-Derived Neurotrophic Factor; Cell Cycle; Cell Surface Receptors; Cells; Chronic; Cognitive deficits; Contusions; Cyclin-Dependent Kinases; Cyclins; Data; Development; Diabetes Mellitus; Diffuse; Drug Targeting; Early treatment; Effectiveness; Enterobacteria phage P1 Cre recombinase; Epidemic; Epitopes; Event; Functional disorder; Genes; Genetic; Glial Fibrillary Acidic Protein; Growth Factor Receptors; Healthcare; Heart Diseases; Hour; Hyperalgesia; ITGAM gene; In Vitro; Injury; Institute of Medicine (U.S.); Intervention; Knock-in Mouse; Knockout Mice; Length; Location; Malignant Neoplasms; Mediating; Medicine; Messenger RNA; Microarray Analysis; Microglia; Modeling; Molecular Target; Motor; Mus; Neuronal Plasticity; Neurons; Neuropathy; Neurotrophic Tyrosine Kinase Receptor Type 2; Nociception; Pain; Pathway interactions; Patients; Peptides; Phosphotransferases; Population; Process; Productivity; Protein Isoforms; Public Health; RNA Splicing; Recovery; Recovery of Function; Regulation; Reporting; Research; Resistance; Role; Seminal; Sensory; Signal Transduction; Simulate; Spinal; Spinal Cord; Spinal Cord Plasticity; Spinal Injuries; Spinal cord injured survivor; Spinal cord injury; Spinal cord injury patients; Stimulus; Symptoms; Testing; Thermal Hyperalgesias; Time; Tissue-Specific Gene Expression; Transgenic Mice; Traumatic Brain Injury; Tropomyosin; Up-Regulation; Western Blotting; Work; allodynia; c-myc Genes; cdc Genes; cell type; central sensitization; chronic pain; conventional therapy; cost; dorsal horn; drug development; experience; flavopiridol; improved; in vivo; inhibitor/antagonist; mechanical allodynia; motor function improvement; mouse model; nestin protein; neuroinflammation; neuron apoptosis; neuronal excitability; new therapeutic target; novel; novel therapeutic intervention; pain receptor; pre-clinical; promoter; receptor; receptor function; response; response to injury; roscovitine; spinal cord injury pain; spontaneous pain; therapeutic target

DESCRIPTION (provided by applicant): Chronic pain is a public health epidemic in the U.S., affecting more than 116 million people and costing greater than $600 billion per year to treat. Spinal cord injury (SCI) results not only in debilitating motor, sensory and cognitive deficits, bu also in a chronic, severe and often unrelenting pain that is largely resistant to conventional treatments (SCI-PAIN). Occurring in as many as 85% of SCI patients, pain starts weeks or months after the original insult, and includes increased pain with noxious stimulation (hyperalgesia), pain in response to previously innocuous stimuli (allodynia), and spontaneous pain. This unremitting pain can be diffuse, bilateral, and usually extends to locations caudal to the spinal injury. The delayed expression of SCI- PAIN and the diffuse localization of painful symptoms suggest that the pathophysiology reflects more than the direct effects at the denervated spinal segments. Indeed, these features of SCI-PAIN strongly suggest the occurrence of maladaptive plasticity in the spinal dorsal horn. An important focus for drug development has been to identify new therapeutic targets/molecules that participate in the spinal cord plasticity associated with the persistence of SCI-PAIN. One promising new therapeutic target, Brain-derived Neurotrophic Factor (BDNF), modulates nociception in the spinal cord. BDNF exerts its effects on nociceptive processing by binding to its full-length, cell surface receptor tropomyosin-related kinase B (trkB.FL) and initiating intracellular signaling. In addition to trkB.FL, the trkB locus also produces a widely-expressed alternatively-spliced truncated isoform, trkB.T1, but the function of this receptor isoform in nociception is largely unknown. TrkB.T1 is upregulated in several non-pain and pain related pathological states and we have reported that the genetic deletion of this receptor in mouse provides significant protection from the development of thermal hyperalgesia and mechanical allodynia across several models of chronic pain. Crucial to this proposal, we have preliminary data showing that trkB.T1 deletion results in significantly improved locomoter recovery and reduced allodynia in a moderate contusion injury mouse model of SCI developed by our group. We conducted differential gene expression studies to examine the potential transcriptional mechanisms regulating these improvements, and found that upregulation of key cell cycle genes correlating with neuronal apoptosis after experimental SCI, are not upregulated in the trkB.T1 null spinal cord. These results suggest that trkB.T1 may be an exciting new molecular target. In this study, we will systematically evaluate, using in vitro and in vivo approaches, whether trkB.T1 regulation of cell cycle genes contributes to SCI-PAIN and determine whether targeting cell cycle genes or trkB.T1, separately or in combination for enhanced effectiveness, can be utilized to develop novel therapeutic interventions to reduce or ameliorate SCI-PAIN.

描述（由申请人提供）：慢性疼痛是美国公共卫生流行病，影响超过1.16亿人，每年花费超过6000亿美元用于治疗。脊髓损伤（SCI）不仅导致衰弱的运动、感觉和认知缺陷，而且还导致慢性、严重和经常持续的疼痛，这种疼痛在很大程度上对传统治疗（SCI- pain）有抵抗力。在多达85%的SCI患者中，疼痛在最初的损伤发生数周或数月后开始，包括有害刺激（痛觉过敏）引起的疼痛增加，对先前无害刺激（异常性疼痛）的疼痛反应，以及自发疼痛。这种持续的疼痛可以是弥漫性的，双侧的，通常延伸到脊髓损伤的尾端。SCI- PAIN的延迟表达和疼痛症状的弥漫性定位表明，病理生理学反映的不仅仅是对失神经脊髓节段的直接影响。事实上，SCI-PAIN的这些特征强烈提示脊髓背角存在适应性不良的可塑性。药物开发的一个重要焦点是确定参与与SCI-PAIN持续性相关的脊髓可塑性的新的治疗靶点/分子。脑源性神经营养因子（BDNF）是一个很有前景的新治疗靶点，它可以调节脊髓中的伤害感受。BDNF通过与其全长细胞表面受体原肌球蛋白相关激酶B （trkB.FL）结合并启动细胞内信号传导，在伤害性加工中发挥作用。除了trkB。在FL中，trkB位点也产生广泛表达的选择性剪接截断异构体trkB。T1，但这种受体异构体在伤害感觉中的功能在很大程度上是未知的。TrkB。T1在几种非疼痛和疼痛相关的病理状态中上调，我们已经报道了该受体的基因缺失在几种慢性疼痛模型中对热痛觉过敏和机械异常性疼痛的发展提供了显著的保护。对这个提议至关重要的是，我们有初步数据显示trkB。在我们组建立的中度挫伤小鼠模型中，T1缺失显著改善了运动恢复，减少了异常性疼痛。我们进行了差异基因表达研究，以研究调节这些改善的潜在转录机制，发现与实验性脊髓损伤后神经元凋亡相关的关键细胞周期基因的上调在trkB中没有上调。T1空脊髓。这些结果表明trkB。T1可能是一个令人兴奋的新分子靶点。在本研究中，我们将使用体外和体内方法系统地评估trkB是否。细胞周期基因的T1调控有助于SCI-PAIN，并确定是靶向细胞周期基因还是靶向trkB。单独或联合使用T1可用于开发新的治疗干预措施，以减少或改善SCI-PAIN。