ITC: Isothermal Titration Calorimeter

ITC：等温滴定量热计

• 批准号: 8246922
• 负责人: Robert Thompson Clubb
• 金额: $ 13.59万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246922
• 关键词: Affinity; Alzheimer' s Disease; Binding; Biochemistry; Biogenesis; Biological Process; Communities; Complex; Disease; Dissociation; Doctor of Philosophy; Embryonic Development; Ensure; Entropy; Funding; Generations; Label; Measures; MicroRNAs; Nucleic Acids; Pathogenesis; Proteins; Research; Research Personnel; Scientist; Services; System; Technology; Thermodynamics; Titrations; Trichomonas Infections; United States National Institutes of Health; Wages; amyloid formation; cancer gene expression; enthalpy; experience; instrument; member; research study; stoichiometry

DESCRIPTION (provided by applicant): A group of NIH supported investigators requests funding to purchase an isothermal titration calorimeter (ITC). The instrument will be used to probe the thermodynamics of protein and nucleic acid complexes involved in a number of important biological processes and diseases, including among others: microRNA biogenesis, embryogenesis, bacterial pathogenesis, amyloid formation, trichomoniasis, Alzheimer's disease, cancer and gene expression. The microcalorimeter will be located in the UCLA-DOE Biochemistry Facility where it will be accessible to the entire UCLA research community. This unique technology is a label-free, rapid and reliable means to obtain thermodynamic information about complex formation. The information obtained includes the binding stoichiometry, affinity, enthalpy and entropy. Access to this technology is lacking at UCLA as the campus contains only a single older-generation microcalorimeter whose access by UCLA scientists is limited. The specific instrument we are requesting is an iTC200 produced by MicroCal, Inc. (Northhampton, MA). It represents the state-of-the-art in ITC technology and has unparalleled sensitivity that enables experiments to be performed with up to 7-fold less material as compared to older-generation instruments. The iTC200 is also capable of measuring a broad range of interactions with sub-micromolar to nanomolar dissociation constants. Thus, it is well suited to serve the needs of the broad user community at UCLA. An experienced Ph.D.-level staff member with salary support from UCLA will be responsible for operating and maintaining the instrument. UCLA will also provide funds to service the instrument, which combined with an effective recharge system, will ensure that the microcalorimeter is productively used.

描述（由申请人提供）：一组NIH支持的研究人员要求资助购买等温滴定量热仪（ITC）。该仪器将用于探测蛋白质和核酸复合物的热力学，这些蛋白质和核酸复合物涉及许多重要的生物过程和疾病，其中包括：微小RNA生物发生、胚胎发生、细菌发病机制、淀粉样蛋白形成、滴虫病、阿尔茨海默病、癌症和基因表达。微量热仪将位于加州大学洛杉矶分校能源部生物化学设施，在那里它将是整个加州大学洛杉矶分校的研究社区访问。这种独特的技术是一种无标记，快速和可靠的手段，以获得有关复杂的形成热力学信息。所获得的信息包括结合化学计量、亲和力、焓和熵。加州大学洛杉矶分校缺乏这项技术，因为校园里只有一台老一代的微量热计，加州大学洛杉矶分校的科学家使用它的机会有限。我们要求的特定仪器是MicroCal，Inc.生产的iTC 200。（Northhampton，MA）。它代表了最先进的ITC技术，具有无与伦比的灵敏度，与老一代仪器相比，可以用少7倍的材料进行实验。iTC 200还能够测量亚微摩尔至纳摩尔解离常数的广泛相互作用。因此，它非常适合满足UCLA广大用户社区的需求。经验丰富的博士-一个级别的工作人员与加州大学洛杉矶分校的工资支持将负责操作和维护仪器。加州大学洛杉矶分校还将提供资金，以服务的仪器，这与一个有效的充电系统相结合，将确保微量热计是富有成效的使用。