Antibacterial and anti-inflammatory activities of Parotid Secretory Protein
腮腺分泌蛋白的抗菌和抗炎活性
基本信息
- 批准号:8468676
- 负责人:
- 金额:$ 33.43万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-06-22 至 2015-05-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAffectAgglutinationAgglutininsAmino Acid SequenceAnti-Bacterial AgentsAnti-Inflammatory AgentsAnti-inflammatoryBacteriaBindingBiochemicalBiologicalBiological ProcessCellsClinicalComputer SimulationDataDental cariesDiseaseDuctal Epithelial CellEndotoxinsEpithelialEpithelial CellsEquilibriumExhibitsFamilyFoundationsFutureGingivaGingivitisGoalsHost DefenseHumanImmune systemIn VitroInfectionInfection ControlInflammationInflammatoryLocationMajor salivary gland structureMicrobeMicrobial BiofilmsModelingMucosal ImmunityMusOralOral cavityOral healthOral mucous membrane structureParotid GlandPeptide Sequence DeterminationPeptidesPeriodontitisPlayPorphyromonas gingivalisPost-Translational Protein ProcessingProtein BindingProteinsResearchRoleSalivaSalivary GlandsSalivary ProteinsSepsisTNF geneTestingTranslational Researchantimicrobialbactericidal permeability increasing proteinbactericidebasecombatcost effectivecytokinedesignin vitro activityin vivoin vivo Modelinnovationlipopolysaccharide-binding proteinnoveloral infectionoral tissueparotid secretory proteinpathogenprotein expressionpublic health relevancerecombinant peptideresearch studyresponsesecretory proteinsynthetic peptide
项目摘要
DESCRIPTION (provided by applicant): The long-term goal of this project is to determine if Parotid Secretory Protein (PSP; SPLUNC2) is a dual function host-defense protein in the oral cavity. PSP is expressed in salivary glands, oral epithelial cells and saliva but its function is unknown. Expression of PSP is up-regulated by bacteria and TNF and an in silico structural analysis of PSP revealed that it may be similar to bactericidal/permeability increasing protein (BPI) and LPS-binding protein (LBP). Guided by the location of active domains in these two proteins, several PSP peptides were designed and found to exhibit both antibacterial and anti-endotoxin activities in vitro and in vivo. Based on these initial results with PSP peptides and additional preliminary data for intact PSP it is hypothesized that the dual- function antibacterial and anti-inflammatory activities detected for PSP peptides are reflected in the biological activity of the intact PSP protein. Two specific aims will test the proposed functions of PSP. Aim 1 will characterize the antibacterial activity of PSP. PSP peptides and recombinant PSP will be used in biochemical, cell biological and in vivo experiments to determine if PSP exhibits bacteriostatic or bactericidal activity, bacterial agglutination, and inhibition of biofilm formation. Aim 2 will characterize the anti- inflammatory activity of PSP. PSP peptides and recombinant PSP will be used in biochemical, cell biological and in vivo experiments to determine if PSP binds LPS, blocks the action of LPS in vitro and protects mice in an LPS-induced sepsis model. If confirmed, the proposed functions of PSP will expand the group of antimicrobial host- defense proteins that are expressed in the oral cavity and participate in the defense of the oral mucosa. Thus, the research proposed here will lay the foundation for future translational research aimed at novel clinical approaches to combat infection and control inflammation.
描述(由申请人提供):该项目的长期目标是确定腮腺分泌蛋白(PSP;SPLUNC2)是否是口腔中的双重功能宿主防御蛋白。 PSP在唾液腺、口腔上皮细胞和唾液中表达,但其功能尚不清楚。 PSP 的表达受到细菌和 TNF 的上调,PSP 的计算机结构分析表明,它可能与杀菌/通透性增加蛋白 (BPI) 和 LPS 结合蛋白 (LBP) 相似。在这两种蛋白质中活性结构域的位置指导下,设计了几种 PSP 肽,并发现它们在体外和体内均表现出抗菌和抗内毒素活性。基于 PSP 肽的这些初步结果和完整 PSP 的额外初步数据,假设检测到的 PSP 肽的双功能抗菌和抗炎活性反映在完整 PSP 蛋白的生物活性中。两个具体目标将测试 PSP 的拟议功能。目标 1 将表征 PSP 的抗菌活性。 PSP肽和重组PSP将用于生化、细胞生物学和体内实验,以确定PSP是否表现出抑菌或杀菌活性、细菌凝集和抑制生物膜形成。目标 2 将表征 PSP 的抗炎活性。 PSP 肽和重组 PSP 将用于生化、细胞生物学和体内实验,以确定 PSP 是否与 LPS 结合、在体外阻断 LPS 的作用并在 LPS 诱导的败血症模型中保护小鼠。如果得到证实,PSP 的拟议功能将扩大在口腔中表达并参与口腔粘膜防御的抗菌宿主防御蛋白组。因此,这里提出的研究将为未来旨在对抗感染和控制炎症的新临床方法的转化研究奠定基础。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Lysine substitutions convert a bacterial-agglutinating peptide into a bactericidal peptide that retains anti-lipopolysaccharide activity and low hemolytic activity.
- DOI:10.1016/j.peptides.2012.03.017
- 发表时间:2012-06
- 期刊:
- 影响因子:3
- 作者:Abdolhosseini M;Nandula SR;Song J;Hirt H;Gorr SU
- 通讯作者:Gorr SU
Human parotid secretory protein is a lipopolysaccharide-binding protein: identification of an anti-inflammatory peptide domain.
- DOI:10.1007/s11010-011-0991-2
- 发表时间:2012-01
- 期刊:
- 影响因子:4.3
- 作者:Abdolhosseini, Mahsa;Sotsky, Julie B.;Shelar, Anuradha P.;Joyce, Paul B. M.;Gorr, Sven-Ulrik
- 通讯作者:Gorr, Sven-Ulrik
A D-enantiomer of the antimicrobial peptide GL13K evades antimicrobial resistance in the Gram positive bacteria Enterococcus faecalis and Streptococcus gordonii.
- DOI:10.1371/journal.pone.0194900
- 发表时间:2018
- 期刊:
- 影响因子:3.7
- 作者:Hirt H;Hall JW;Larson E;Gorr SU
- 通讯作者:Gorr SU
Bio-inspired stable antimicrobial peptide coatings for dental applications.
- DOI:10.1016/j.actbio.2013.06.017
- 发表时间:2013-09
- 期刊:
- 影响因子:9.7
- 作者:Holmberg, Kyle V.;Abdolhosseini, Mahsa;Li, Yuping;Chen, Xi;Gorr, Sven-Ulrik;Aparicio, Conrado
- 通讯作者:Aparicio, Conrado
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
SVEN-ULRIK GORR其他文献
SVEN-ULRIK GORR的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('SVEN-ULRIK GORR', 18)}}的其他基金
Antibacterial and anti-inflammatory activities of Parotid Secretory Protein
腮腺分泌蛋白的抗菌和抗炎活性
- 批准号:
8269569 - 财政年份:2010
- 资助金额:
$ 33.43万 - 项目类别:
Antibacterial and anti-inflammatory activities of Parotid Secretory Protein
腮腺分泌蛋白的抗菌和抗炎活性
- 批准号:
8097392 - 财政年份:2010
- 资助金额:
$ 33.43万 - 项目类别:
Systems Approach to Sjogren's Syndrome Pathogenesis (SASSP)
干燥综合征发病机制的系统方法 (SASSP)
- 批准号:
7530422 - 财政年份:2009
- 资助金额:
$ 33.43万 - 项目类别:
Systems Approach to Sjogren's Syndrome Pathogenesis (SASSP)
干燥综合征发病机制的系统方法 (SASSP)
- 批准号:
7817012 - 财政年份:2009
- 资助金额:
$ 33.43万 - 项目类别:
SELECTIVE AGGREGATION AND SORTING OF SALIVARY PROTEINS
唾液蛋白的选择性聚集和排序
- 批准号:
2908097 - 财政年份:1999
- 资助金额:
$ 33.43万 - 项目类别:
SELECTIVE AGGREGATION AND SORTING OF SALIVARY PROTEINS
唾液蛋白的选择性聚集和排序
- 批准号:
6379803 - 财政年份:1999
- 资助金额:
$ 33.43万 - 项目类别:
SELECTIVE AGGREGATION AND SORTING OF SALIVARY PROTEINS
唾液蛋白的选择性聚集和排序
- 批准号:
6176851 - 财政年份:1999
- 资助金额:
$ 33.43万 - 项目类别:
相似海外基金
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
- 批准号:
BB/Z514391/1 - 财政年份:2024
- 资助金额:
$ 33.43万 - 项目类别:
Training Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
- 批准号:
2312555 - 财政年份:2024
- 资助金额:
$ 33.43万 - 项目类别:
Standard Grant
RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
- 批准号:
2327346 - 财政年份:2024
- 资助金额:
$ 33.43万 - 项目类别:
Standard Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
- 批准号:
ES/Z502595/1 - 财政年份:2024
- 资助金额:
$ 33.43万 - 项目类别:
Fellowship
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
- 批准号:
23K24936 - 财政年份:2024
- 资助金额:
$ 33.43万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
- 批准号:
ES/Z000149/1 - 财政年份:2024
- 资助金额:
$ 33.43万 - 项目类别:
Research Grant
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
- 批准号:
2901648 - 财政年份:2024
- 资助金额:
$ 33.43万 - 项目类别:
Studentship
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
- 批准号:
488039 - 财政年份:2023
- 资助金额:
$ 33.43万 - 项目类别:
Operating Grants
New Tendencies of French Film Theory: Representation, Body, Affect
法国电影理论新动向:再现、身体、情感
- 批准号:
23K00129 - 财政年份:2023
- 资助金额:
$ 33.43万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Protruding Void: Mystical Affect in Samuel Beckett's Prose
突出的虚空:塞缪尔·贝克特散文中的神秘影响
- 批准号:
2883985 - 财政年份:2023
- 资助金额:
$ 33.43万 - 项目类别:
Studentship