Bio-inspired stable antimicrobial peptide coatings for dental applications.
Bio-inspired stable antimicrobial peptide coatings for dental applications.
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DOI：
10.1016/j.actbio.2013.06.017
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发表时间：
2013-09
影响因子：
9.7
通讯作者：
Aparicio, Conrado
中科院分区：
文献类型：
--
作者：
Holmberg, Kyle V.;Abdolhosseini, Mahsa;Li, Yuping;Chen, Xi;Gorr, Sven-Ulrik;Aparicio, Conrado
我们开发了一种新型钛涂层，可用于预防牙科和骨科中与感染相关的植入物失效。该涂层含有一种抗菌肽GL 13 K，该肽来源于腮腺分泌蛋白，先前已证明其在溶液中具有杀菌和抑菌作用。我们的特点是产生的物理化学性质，耐降解，对牙龈卟啉单胞菌的活性，并在体外细胞相容性。牙龈卟啉单胞菌是与牙种植体周围炎相关的病原体，牙种植体周围炎是对细菌的炎症反应，导致骨丢失和种植体失败。我们的表面改性获得了均匀的，高度疏水的，和强锚定的GL 13 K涂层，耐机械，热化学和酶降解。GL 13 K涂层具有杀菌作用，因此与对照表面相比，显著减少了活细菌的数量。最后，成骨细胞和人牙龈成纤维细胞的充分增殖证明了GL 13 K涂层的细胞相容性。GL 13 K-生物功能化钛的稳健性、抗微生物活性和细胞相容性使其成为持续抑制细菌生物膜生长的有希望的候选物。这种表面化学为开发多功能生物活性表面提供了基础，以降低患者发病率并提高金属牙科和骨科植入物的长期临床疗效。
We developed a novel titanium coating that has applications for preventing infection-related implant failures in dentistry and orthopedics. The coating incorporates an antimicrobial peptide, GL13K, derived from parotid secretory protein, which has been previously shown to be bactericidal and bacteriostatic in solution. We characterized the resulting physicochemical properties, resistance to degradation, activity against Porphyromonas gingivalis, and in vitro cytocompatibility. P. gingivalis is a pathogen associated with dental peri-implantitis, an inflammatory response to bacteria resulting in bone loss and implant failure. Our surface modifications obtained a homogeneous, highly hydrophobic, and strongly-anchored GL13K-coating that was resistant to mechanical, thermochemical, and enzymatic degradation. The GL13K-coatings had bactericidal effect and thus, significantly reduced the number of viable bacteria compared to control surfaces. Finally, adequate proliferation of osteoblasts and human-gingival-fibroblasts demonstrated the GL13K-coating’s cytocompatibility. The robustness, antimicrobial activity, and cytocompatibility of GL13K-biofunctionalized titanium make it a promising candidate for sustained inhibition of bacterial biofilm growth. This surface chemistry provides a basis for development of multifunctional bioactive surfaces to reduce patient morbidities and improve long-term clinical efficacy of metallic dental and orthopedic implants.
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DOI：
10.1016/j.colsurfb.2013.02.005
发表时间：
2013-07-01
期刊：
Colloids and surfaces. B, Biointerfaces
影响因子：
--
作者：
Chen X;Sevilla P;Aparicio C
通讯作者：
Aparicio C
影响因子：
3.9
作者：
Gorr, Sven-Ulrik;Abdolhosseini, Mahsa;Sotsky, Julie
通讯作者：
Sotsky, Julie
影响因子：
6.7
作者：
Hallstrom, Hadar;Persson, G. Rutger;Renvert, Stefan
通讯作者：
Renvert, Stefan
影响因子：
4.6
作者：
Myshin, HL;Wiens, JP
通讯作者：
Wiens, JP
影响因子：
14
作者：
Kazemzadeh-Narbat, Mehdi;Kindrachuk, Jason;Wang, Rizhi
通讯作者：
Wang, Rizhi