Regulatory Mechanisms Controlling Expression of P. gingivalis Surface Structures

控制牙龈卟啉单胞菌表面结构表达的调控机制

基本信息

  • 批准号:
    8448546
  • 负责人:
  • 金额:
    $ 35.62万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2009
  • 资助国家:
    美国
  • 起止时间:
    2009-05-01 至 2015-08-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Periodontitis is a biofilm-mediated disease that afflicts 35% of the adult population in the US, and persistent infections are associated with systemic disease, including cardiovascular disease and stroke. The proliferation of anaerobic bacteria in the subgingival crevice is central to progression of this chronic disease, with Porphyromonas gingivalis being implicated as one of the primary pathogens. We recently reported that a loss in K-antigen capsule synthesis enhanced biofilm formation in P. gingivalis, hence regulation of this virulence factor plays a key role in pathogenicity and biofilm formation. During preliminary studies, we discovered that a gene with high similarity to the DNA binding protein HU (PG0121) is transcribed with the K- antigen capsule synthesis operon and that this "histone-like" regulatory protein controls expression of the capsule operon. We are now poised to determine the regulatory mechanisms controlling expression of K- antigen capsule. The long-term goal of our research is to elucidate the regulatory mechanisms and signals that control the expression of genes involved in modifying the surface properties of P. gingivalis, and to determine how changes in expression of these genes relate to biofilm development and virulence. The objectives of this application are to characterize the role of HU protein in the synthesis of K-antigen capsule and to investigate the role of the two HU subunits (PG0121 and PG1258) in regulating global gene expression using chromatin immunoprecipitation and microarray analysis. The research proposed in this application is significant because understanding the control of surface property changes is a vital link to understanding the switch this bacterium makes from a quiescent state as a commensal to a virulent pathogen. As an outcome of these studies, we will have established how HU a global regulatory protein controls expression of a key virulence factor, K-antigen capsule. This information will lead to a better understanding of the regulatory networks that either direct P. gingivalis to become a virulent pathogen or to continue to lie low and persist. Our results will potentially lead to the development of new therapeutic strategies for modulating biofilm formation by this oral pathogen.
描述(申请人提供):牙周炎是一种生物膜介导的疾病,困扰着美国35%的成年人口,持续感染与系统性疾病有关,包括心血管疾病和中风。牙龈下缝隙中厌氧细菌的增殖是这种慢性疾病进展的核心,牙龈卟啉单胞菌被认为是主要病原体之一。我们最近报道K抗原被膜合成的缺失促进了牙龈假单胞菌生物被膜的形成,因此对这一毒力因子的调节在致病性和生物被膜形成中起着关键作用。在初步研究中,我们发现一个与DNA结合蛋白HU(PG0121)高度相似的基因是由K抗原衣壳合成操纵子转录的,并且这种类似组蛋白的调节蛋白控制着衣壳操纵子的表达。我们现在准备确定控制K抗原胶囊表达的调控机制。我们研究的长期目标是阐明调控牙龈假单胞菌表面特性基因表达的调控机制和信号,并确定这些基因表达的变化与生物膜发育和毒力的关系。本研究的目的是通过染色质免疫沉淀和微阵列分析,研究HU蛋白在K抗原被膜合成中的作用,以及HU的两个亚基(PG0121和PG1258)在调节全球基因表达中的作用。在这一申请中提出的研究具有重要意义,因为了解表面属性变化的控制是理解这种细菌从静止状态作为共生菌向毒力病原体转变的关键环节。作为这些研究的结果,我们将确定HU是如何控制关键毒力因子K抗原胶囊的表达的。这些信息将有助于更好地理解调控网络,这些网络要么引导牙龈假单胞菌成为一种毒力病原体,要么继续保持低调并持续存在。我们的结果可能会导致开发新的治疗策略来调节这种口腔病原体的生物膜形成。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Mary Ellen Davey其他文献

Inhibition of SARS-CoV-2 infection by emPorphyromonas gingivalis/em and the oral microbiome
牙龈卟啉单胞菌/和口腔微生物群对 SARS-CoV-2 感染的抑制作用
  • DOI:
    10.1128/spectrum.00599-24
  • 发表时间:
    2024-08-21
  • 期刊:
  • 影响因子:
    3.800
  • 作者:
    Alexander Bontempo;Alexandra Chirino;Alireza Heidari;Alexandra Lugo;Satoru Shindo;Maria R. Pastore;Riccardo Madonia;Sibel A. Antonson;Cristina Godoy;Frank C. Nichols;Jan Potempa;Mary Ellen Davey;Toshihisa Kawai;Mark J. Cayabyab
  • 通讯作者:
    Mark J. Cayabyab

Mary Ellen Davey的其他文献

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{{ truncateString('Mary Ellen Davey', 18)}}的其他基金

L-Arg availability affects the physiological state of porphyromonas gingivalis.
L-精氨酸的可用性影响牙龈卟啉单胞菌的生理状态。
  • 批准号:
    10649693
  • 财政年份:
    2022
  • 资助金额:
    $ 35.62万
  • 项目类别:
Microbial sphingolipids and suppression of host inflammation in periodontal disease
微生物鞘脂和牙周病宿主炎症的抑制
  • 批准号:
    10435569
  • 财政年份:
    2021
  • 资助金额:
    $ 35.62万
  • 项目类别:
Microbial sphingolipids and suppression of host inflammation in periodontal disease
微生物鞘脂和牙周病宿主炎症的抑制
  • 批准号:
    10314304
  • 财政年份:
    2021
  • 资助金额:
    $ 35.62万
  • 项目类别:
Microbial sphingolipids and suppression of host inflammation in periodontal disease
微生物鞘脂和牙周病宿主炎症的抑制
  • 批准号:
    10640238
  • 财政年份:
    2021
  • 资助金额:
    $ 35.62万
  • 项目类别:
L-Arg availability affects the physiological state of porphyromonas gingivalis
L-精氨酸的可用性影响牙龈卟啉单胞菌的生理状态
  • 批准号:
    10316786
  • 财政年份:
    2015
  • 资助金额:
    $ 35.62万
  • 项目类别:
Regulatory Mechanisms Controlling Expression of P. gingivalis Surface Structures
控制牙龈卟啉单胞菌表面结构表达的调控机制
  • 批准号:
    9986131
  • 财政年份:
    2015
  • 资助金额:
    $ 35.62万
  • 项目类别:
L-Arg Availability Affects the Physiological State of Porphyromonas gingivalis
L-精氨酸可用性影响牙龈卟啉单胞菌的生理状态
  • 批准号:
    8886720
  • 财政年份:
    2015
  • 资助金额:
    $ 35.62万
  • 项目类别:
L-Arg Availability Affects the Physiological State of Porphyromonas gingivalis
L-精氨酸可用性影响牙龈卟啉单胞菌的生理状态
  • 批准号:
    9011518
  • 财政年份:
    2015
  • 资助金额:
    $ 35.62万
  • 项目类别:
Regulatory Mechanisms Controlling Expression of P. gingivalis Surface Structures
控制牙龈卟啉单胞菌表面结构表达的调控机制
  • 批准号:
    9765046
  • 财政年份:
    2015
  • 资助金额:
    $ 35.62万
  • 项目类别:
Regulatory Mechanisms Controlling Expression of P. gingivalis Surface Structures
控制牙龈卟啉单胞菌表面结构表达的调控机制
  • 批准号:
    8963710
  • 财政年份:
    2015
  • 资助金额:
    $ 35.62万
  • 项目类别:

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