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Genotyping and DNA/RNA Analysis

基因分型和 DNA/RNA 分析

基本信息

批准号：
8378259
负责人：
DANIELLE Renee REED
金额：
$ 13.66万
依托单位：
MONELL CHEMICAL SENSES CENTER
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8378259
关键词：
Address Algorithms Alleles Animals Area Base Sequence Behavior Binding Biology Chemicals Complementary DNA Complex Copy Number Polymorphism DNA DNA Sequence DNA analysis Data Data Analyses Dinucleotide Repeats Discipline Discrimination Dyes Effectiveness Electrophoresis Engineering Ensure Equipment Fostering Funding Gel Genetic Polymorphism Genetic Variation Genome Genotype Goals Histocompatibility Testing Housing Human Imagery Individual Institutes Knock-out Knowledge Label Laboratories Laboratory Study Light Measurement Measures Messenger RNA Methods Microsatellite Repeats Minor Mission Molecular Biology Mus National Institute on Deafness and Other Communication Disorders Persons Physiology Procedures Proteins Psychophysics Public Health RNA RNA analysis Rattus Relative (related person)Research Research Methodology Research Personnel Research Project Grants Resources Scientific Advances and Accomplishments Sensory Sensory Physiology Sepharose Services Spectrophotometry Structure Students System Taste Blindness Teaching Method Techniques Time Tissues Training Transgenic Mice United States National Institutes of Health Variant Work base cell type college dalton design ds-DNA experience flexibility high school insertion/deletion mutation instrument instrumentation interest meetings polyacrylamide postdoctoral investigator programs prototype repaired research study

项目摘要

The primary expertise of Monell investigators spans a wide range of disciplines, including human sensory physiology and psychophysics (Drs. Breslin, Cowart, Dalton, Lundstrom, Pelchat, Mennella, Wysocki. Yamazaki. and Zhao), mouse and/or rat genefics (Drs. Bachmanov, Reed, and Tordoff), molecular biology (Drs. Brand, Margolskee, and Huang), and animal chemosensory physiology (Drs. Beauchamp, Lowe, Margolskee, and Reisert). Most or all of these research programs use DNA or RNA analysis methods at various fimes, but these methods often lie outside their area of expertise. This Core will serve to remove barriers from the invesfigators' use of these techniques. For instance, transgenic mice used by laboratories that study mouse behavior and physiology need to be genotyped to ensure that they are of the correct group (e.g., knockout or wild-type), but the invesfigator has no training in genotyping methods. Therefore, it is useful for the investigator to have a technician available who has been rigorously trained by experts to perform this work, to make use ofthe equipment available in the Core. Thus, not all people who study genefically engineered mice need to have all the equipment to genotype mice in their own laboratories. Likewise, invesfigators doing human sensory work might like to know their subject's genotype for key sensory polymorphisms (e.g., is the person homozygous for certain alleles associated with bitter taste blindness?). Again, this is often outside the expertise of their laboratory, but by working with this Core they can receive appropriate training and readily collect these data. Thus, this Core will provide a centralized common resource and reservoir of experience in genotyping and DNA/RNA analysis that would be inefficient to replicate in each individual laboratory. The availability of Core services extends the capabilifies of the participafing laboratories beyond what each could do individually.

莫奈尔的主要专长