Measurement of body composition in rats and mice

大鼠和小鼠身体成分的测量

• 批准号: 8053557
• 负责人: DANIELLE Renee REED
• 金额: $ 17.61万
• 依托单位: MONELL CHEMICAL SENSES CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8053557
• 关键词: Alcoholism; Alcohols; Animal Model; Animals; Area; Body Composition; Body Weight; Breeding; Calcium; Calories; Chemicals; DEXA; Desire for food; Diabetes Mellitus; Diet; Disease; Eating; Equipment; Equipment Design; Fatty acid glycerol esters; Funding; Genetic; Genetic Variation; Genetically Engineered Mouse; Inflammation; Intake; Measurement; Measures; Methods; Minerals; Mouse Strains; Movement; Mus; Muscle; Nutrient; Obesity; Rattus; Request for Applications; Research; Research Personnel; System; Taste Perception; Tissue Sample; Translational Research; Tube; United States National Institutes of Health; Water; Work; awake; detection of nutrient; genetic manipulation; human disease; meetings; obesity prevention; preference; public health relevance

DESCRIPTION (provided by applicant): Many types of research require accurate measures of body composition. In this application, we are requesting funding to purchase equipment designed to measure body composition (fat, muscle, and water). This equipment will meet the needs of thirteen National Institutes of Health- funded projects conducted by eight investigators at the Monell Chemical Senses Center. Currently, body composition is measured at Monell by chemical analysis, which is slow, or by DEXA, which is limited to mice and requires anesthetization; or we make use of nearby equipment that necessitates sacrifice of animals after measurements are taken. The advantages of the system requested in this application are that (1) it measures composition in tissue samples or in awake animals of several species (by use of animal tubes that restrict movement), (2) it is faster and more accurate than the other methods, and (3) it can measure the animals while they are onsite, without need to sacrifice them afterward. The projects that would benefit from measuring body composition involve the genetics of obesity in mice and rats; alcohol preference among selectively bred strains of mice; the interaction of taste and obesity, food intake, and body weight, especially in mice that are "sweet-liker's"; the appetite for minerals such as calcium; the effect of inflammation on taste; and genetically engineered mice with compromised or enhanced taste function or with deficits in gut nutrient sensing. This requested equipment will enhance the quality of the work done by a large core group of investigators. Translational research in these areas is of critical concern because of the focus on obesity prevention and other diseases of nutrient intake, e.g., alcoholism, excess sweet intake, and diabetes. Public Health Relevance: The body composition of animals can differ because of naturally-occurring genetic variation, genetic manipulation, or other treatments, e.g., a diet high in fat or calories. The fast and accurate measurement of body composition in animals enhances the quality and reliability of experimental results. To that end, we are requesting funds to purchase a Bruker minispec LF110 system that will measure the amount of fat, muscle, and water in animal models of human disease.

描述（由申请人提供）：许多类型的研究需要准确的身体组成措施。在此应用中，我们要求资金购买旨在测量人体成分（脂肪，肌肉和水）的设备。该设备将满足13个国立卫生研究院基金会项目的需求，该项目由Monell Chemical Senses Center的8名调查人员进行。当前，通过化学分析或通过dexa进行的化学分析测量身体成分，该化学分析仅限于小鼠，需要麻醉。或者，我们利用附近的设备，在进行测量后需要牺牲动物。在本应用中要求的系统的优点是（1）它测量组织样品中的组成或几种物种的清醒动物（通过使用限制运动的动物管），（2）它比其他方法更快，更准确，并且（3）它可以在动物的同时进行牺牲，而无需牺牲它们，而无需牺牲它们。测量身体成分受益的项目涉及小鼠和大鼠肥胖的遗传学；选择性繁殖小鼠菌株之间的酒精偏好；味道和肥胖，食物摄入量和体重的相互作用，尤其是在“类似甜美的小鼠”中；矿物质（例如钙）的食欲；炎症对味道的影响；以及具有损害或增强的味觉功能或肠道营养感应缺陷的基因工程小鼠。该要求的设备将提高大型核心调查人员所做的工作质量。这些领域的转化研究是至关重要的，因为重点是预防肥胖症和其他营养摄入的疾病，例如酒精中毒，多余的甜食摄入量和糖尿病。 公共卫生相关性：动物的身体组成可能会有所不同，因为天然存在遗传变异，遗传操纵或其他治疗方法，例如脂肪或卡路里含量高的饮食。快速准确地测量动物中的身体成分可增强实验结果的质量和可靠性。为此，我们要求资金购买Bruker Minispec LF110系统，该系统将测量人类疾病动物模型中的脂肪，肌肉和水的量。