权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development and regeneration of the zebrafish maxillary barbel

斑马鱼上颌触须的发育和再生

基本信息

批准号：
8460604
负责人：
ELIZABETH E LECLAIR
金额：
$ 2.21万
依托单位：
DE PAUL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-03-01 至 2014-11-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8460604
关键词：
Abnormal Cell Adult Affect Amputation Apoptosis Area Axon B-Lymphocytes Barbus Behavior Biological Biological Assay Biological Models Biomedical Research Blood Vessels Blood capillaries Caliber Candidate Disease Gene Cell Death Cell Proliferation Cell division Cell physiology Cells Cessation of life Cicatrix Collaborations Connective Tissue Data Deposition Development Electroporation Embryo Environment Epithelial Esthesia Extracellular Matrix Eye Face Fertilization Fishes Fluid Balance Funding Genes Genetic Gland Glypican Goals Growth Health Heart Heat-Shock Response Histocompatibility Testing Histocytochemistry Human Hypertrophic Cicatrix Image In Situ Hybridization Injection of therapeutic agent Injury Institution Investigation Keloid Knowledge Label Life Ligands Longevity Lymph Lymphangiogenesis Lymphatic Maintenance Maps Maxilla Mediating Mesenchymal Methods Molecular Molecular and Cellular Biology Natural regeneration Nature Neoplasm Metastasis Nerve Nerve Regeneration Nose Organ Pathology Pathway interactions Pattern Peripheral Peripheral Nerves Physiological Pigments Positioning Attribute Process Protocols documentation Regulation Research Resolution Reverse Transcriptase Polymerase Chain Reaction Rodent Role Sensory Signal Pathway Signal Transduction Signaling Molecule Skin Structure Surveys System Tadpoles Tail Taste Buds Techniques Testing Tissues Training Training Programs Transgenic Organisms Trauma United States National Institutes of Health Vertebrates Vibrissae Wound Healing Xenopus Zebrafish afferent nerve appendage base blastema capillary cell behavior cell type functional restoration in vivo in vivo Model innovation light microscopy melanocyte migration mutant next generation novel novel strategies receptor repaired research study response response to injury senescence skills tumor

项目摘要

DESCRIPTION (provided by applicant): The zebrafish is a vertebrate animal that can regenerate several adult tissue types. These include well-studied structures such as fins, heart and eyes, but also poorly studied ones, such as the whisker-like facial barbels. The zebrafish maxillary barbel (ZMB) contains skin cells, glands, taste buds, connective tissues, nerves, and vascular components. Because the transparent barbells are external, all of the differentiated cell types within are visible and experimentally accessible. Barbels are known to regenerate in other species of fishes, but have not been studied for this capacity in zebrafish. Based on studies to date, we hypothesize that Wnt signaling molecules and glypican co- receptors are critical for both ZMB outgrowth and regeneration after injury. In our first aim, we will document patterns of cell division and cell death during development and regeneration of this appendage, and confirm the existence and function of the barbel lymphatics, which we are the first to describe. In the second aim, we will 1) map the spatial and temporal resolution of non-canonical Wnt ligands and receptors (glypicans and frizzleds) in ZMB tissue, 2) use transgenic fish to test if restoring glypican function restores ZMB outgrowth in a zebrafish mutant, 3) survey if canonical Wnt signaling is required for ZMB budding and regeneration, and 4) test candidate genes in the ZMB using a morpholino approach. Knowledge gained from these investigations will contribute significantly to our understanding of the genetic interactions involved in the development and maintenance of skin and skin appendages. Because the ZMB is an adult organ that regenerates, this study will also illuminate the response to injury of many terminally differentiated cell types, including blood vessels, lymphatics and nerves. Unlike regeneration in the zebrafish caudal fin, regeneration in the ZMB is characterized by abnormal cell proliferation and extracellular matrix deposition, a process that may be relevant to vertebrate wound healing pathologies such as keloids and/or hypertrophic scars. The ability of zebrafish barbel nerves to regrow axons to their taste bud targets may suggest approaches to restore peripheral sensation to traumatized tissues. Finally, the availability within the ZMB of both a capillary loop and a large, accessible lymphatic is an attractive system in which to study the physiological and pathological functions of the vasculature, including fluid balance and tumor metastasis. )

描述（由申请人提供）：斑马鱼是一种脊椎动物，可以再生几种成体组织类型。这些包括研究得很好的结构，如鳍、心脏和眼睛，但也有研究得很少的结构，如胡须状的面部触须。斑马鱼上颌须（ZMB）含有皮肤细胞、腺体、味蕾、结缔组织、神经和血管成分。因为透明的杠铃是外部的，所以内部所有分化的细胞类型都是可见的，并且可以通过实验获得。已知其他鱼类的触须可以再生，但尚未研究过斑马鱼的这种能力。基于迄今为止的研究，我们假设Wnt信号传导分子和磷脂酰肌醇蛋白聚糖共受体对于损伤后的ZMB生长和再生都是关键的。在我们的第一个目标，我们将文件模式的细胞分裂和细胞死亡的发展和再生过程中，这个附属物，并确认的barbel aperhatics的存在和功能，这是我们第一次描述。在第二个目标中，我们将1）绘制ZMB组织中非典型Wnt配体和受体（磷脂酰肌醇蛋白聚糖和卷曲蛋白聚糖）的空间和时间分辨率，2）使用转基因鱼来测试恢复磷脂酰肌醇蛋白聚糖功能是否恢复斑马鱼突变体中的ZMB生长，3）调查ZMB出芽和再生是否需要典型Wnt信号传导，以及4）使用吗啉代方法测试ZMB中的候选基因。从这些研究中获得的知识将大大有助于我们了解皮肤和皮肤附属物的发育和维持中所涉及的遗传相互作用。由于ZMB是一个可以再生的成人器官，因此这项研究还将阐明许多终末分化细胞类型（包括血管、淋巴管和神经）对损伤的反应。与斑马鱼尾鳍中的再生不同，ZMB中的再生的特征在于异常的细胞增殖和细胞外基质沉积，这是一个可能与脊椎动物伤口愈合病理学如瘢痕疙瘩和/或增生性瘢痕相关的过程。斑马鱼触须神经再生轴突到味蕾目标的能力可能表明恢复创伤组织外周感觉的方法。最后，ZMB内的毛细血管环和大的可接近的淋巴管的可用性是一个有吸引力的系统，其中研究脉管系统的生理和病理功能，包括流体平衡和肿瘤转移。)