CORE C: MICROANALYSIS, SEQUENCING AND INFORMATICS

核心 C：微观分析、测序和信息学

• 批准号: 8377051
• 负责人: MYLENE W YAO
• 金额: $ 20.56万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8377051
• 关键词: 3' Untranslated Regions; Biological; Communities; Data Set; Development; Embryo; Genomics; Germ Cells; Informatics; Microfluidics; Molecular Genetics; Protocols documentation; RNA; RNA Sequences; RNA Splicing; Reproductive Biology; Role; Sampling; Techniques; Technology; Universities; Variant; computerized tools; next generation; novel; preimplantation; single cell analysis; stem cell biology

A. Objective The objective of Core C is to develop and disseminate experimental and computational technologies that are critical for studying the molecular genetics and genomics of small biological samples, as exemplified by germ cell or pre-implantation embryo samples. We will take two main directions to accomplish this objective: Aim 1. Develop experimental protocols to apply massively parallel RNA-sequencing (RNA-Seq) to discover novel, splice and other 3'UTR variants and their differential roles in development, with a special emphasis on understanding the post-transcriptional regulatory function of 3'UTR variants. Aim 2. Host and disseminate validated experimental protocols, computational tools and annotated RNA-Seq data sets to make next-generation sequencing, single-cell analysis, and microfluidics techniques accessible to the Reproductive Biology, SCCPRIR, and broader scientific communities.

A.目的 核心C的目标是开发和传播实验和计算技术， 对于研究小生物样品的分子遗传学和基因组学至关重要，例如细菌 细胞或植入前胚胎样品。我们将从两个主要方向来实现这一目标： 目标1。开发实验方案，将大规模并行RNA测序（RNA-Seq）应用于 发现新的剪接和其他3 'UTR变体及其在发育中的不同作用， 特别强调理解3 'UTR变体的转录后调节功能。 目标2.主持和传播经验证的实验方案、计算工具和注释 用于下一代测序、单细胞分析和微流体技术的RNA-Seq数据集 生殖生物学、SCCPRIR和更广泛的科学界可以使用的技术。