CMPB Central Pathology Support

CMPB 中央病理学支持

• 批准号: 8554221
• 负责人: Robert Sills
• 金额: $ 1069.43万
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8554221
• 关键词: Adult; Anatomy; Atherosclerosis; Autopsy; Biological Assay; Blood; Body Fluids; Cardiovascular Diseases; Cells; Chronic; Clinical; Clinical Pathology; Collaborations; Communities; Consultations; DNA; Disease; Electron Microscopy; Embryo; Freezing; Gene Expression; Gene Expression Regulation; Genetically Engineered Mouse; Genotype; Goals; Histology; Immune; Immunohistochemistry; Incidence; Inflammatory; Kidney Diseases; Kidney Neoplasms; Laboratories; Laboratory Animals; Lasers; Lung diseases; Malignant Neoplasms; Mediating; Medical Research; Methods; Microdissection; Mus; National Institute of Environmental Health Sciences; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Paraffin Embedding; Pathology; Phenotype; Process; Proteins; Proteomics; Protocols documentation; RNA; RNA analysis; Rattus; Research Personnel; Reverse Transcriptase Polymerase Chain Reaction; Services; Skeletal Development; Technical Expertise; Techniques; Testing; Tissue Sample; Tissues; Western Blotting; Work; animal tissue; base; dietary restriction; digital imaging; male; new technology; sample collection

Collaborative and support oriented work has over the past year had an impact in the study, and on the understanding, of a spectrum of diseases and disease processes such as cardiovascular disease (atherosclerosis), inflammatory respiratory disease, obesity and type II diabetes, inflammatory and immune-mediated renal disease, gene expression and gene regulation, axial skeletal development, cancer and transgenerational effects of diet restriction. Additionally, associations between exacerbation of chronic progressive nephropathy in subchronic studies and increased incidence of renal tumors in subsequent 2-year studies was examined for male rats. In addition, the mouse phenotyping group has assisted with the phenotyping a few adult genetically engineered mice projects. While the focus of services provided by this facility has been on the histological assessment with embryonic lethal phenotypes, additional services have been provided in assisting with special necropsy techniques and special applications with regards to RNA isolation. The laser microdissection group has worked with investigators to establish successful protocols for the isolation of protein (western blots and proteomic analysis), RNA from frozen and more recently from laser-microdissected, paraffin-embedded tissue (quantitative RT-PCR) and DNA (embryo genotyping) from laser-microdissected tissue samples. The immunohistochemistry group has worked with investigators to establish immunostaining protocols for a variety of unique tissue markers.

在过去的一年里，以协作和支持为导向的工作对心血管疾病（动脉粥样硬化）、炎症性呼吸系统疾病、肥胖和II型糖尿病、炎症和免疫介导的肾脏疾病、基因表达和基因调控、轴向骨骼发育、癌症和饮食限制的跨代影响等一系列疾病和疾病过程的研究和理解产生了影响。此外，在随后的2年研究中，对雄性大鼠进行了亚慢性研究中慢性进行性肾病的恶化与肾肿瘤发病率增加之间的关联研究。此外，小鼠表型组还协助了一些成年基因工程小鼠项目的表型分析。虽然该设施提供的服务的重点是胚胎致死表型的组织学评估，但在协助特殊尸检技术和RNA分离方面的特殊应用方面也提供了额外的服务。激光显微解剖组与研究人员合作建立了成功的分离蛋白质（western blots和蛋白质组学分析）、冷冻和最近激光显微解剖的RNA、石蜡包埋组织（定量RT-PCR）和激光显微解剖组织样本的DNA（胚胎基因分型）的方案。免疫组织化学小组与研究人员合作建立了各种独特组织标记物的免疫染色方案。