CMPB Central Pathology Support

CMPB 中央病理学支持

• 批准号: 7734576
• 负责人: Robert Sills
• 金额: $ 573.93万
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7734576
• 关键词: Arts; Atherosclerosis; Biological Assay; Biological Markers; Blood; Body Fluids; Cardiovascular Diseases; Cells; Clinical; Clinical Pathology; Collaborations; Communities; Consultations; Disease; Gene Expression; Genes; Goals; Growth Factor; Hormones; Image Analysis; Immune; Industry; Inflammatory; Insulin; Kidney Diseases; Laboratories; Lasers; Leptin; Lung diseases; Mediating; Medical Research; Methods; Microdissection; Molecular Machines; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Pathology; Performance; Population; Process; Regulation; Research Personnel; Scanning; Serum; Skeletal Development; Technical Expertise; Testing; Testosterone; Tissues; Troponin T; Vascular Endothelial Growth Factors; Work; base; cytokine; dietary restriction; digital imaging; fatty acid binding protein; fatty acid-binding proteins; instrument; new technology; sample collection

Collaborative and support oriented work has over the past year had an impact in the study, and on the understanding, of a spectrum of diseases and disease processes such as cardiovascular disease (atherosclerosis), inflammatory respiratory disease, obesity and type II diabetes, inflammatory and immune-mediated renal disease, gene expression and gene regulation, axial skeletal development and transgenerational effects of diet restriction. To keep the clinical laboratory up-to-date, new methods and instruments have been obtained. For example, An Aperio Scan Scope has been added for digital imaging and imaging analysis of tissue sections. A CellCut laser microdissection scope (Molecular Machines and Industries) has been incorporated for the targeting and capture of specific cells or cell populations. Additionally, a SECTOR Imager 2400 by Meso Scale Discovery was obtained for the performance of a variety of serum biomarkers, in single- or multi-plex fashion, including, but not limited to, troponin T and I, fatty acid binding protein 3, inflammatory cytokines (e.g., interlukins, TNFa, INFg), growth factors (e.g., VEGF), hormones (e.g., insulin, leptin, etradiol, progeterone, testosterone).

在过去的一年里，协作和支持导向的工作对研究产生了影响，并对一系列疾病和疾病过程的理解产生了影响，如心血管疾病（动脉粥样硬化），炎症性呼吸道疾病，肥胖和II型糖尿病，炎症和免疫介导的肾脏疾病，基因表达和基因调控，中轴骨骼发育和饮食限制的跨代影响。为了使临床实验室与时俱进，已经获得了新的方法和仪器。例如，增加了Aperio扫描范围，用于组织切片的数字成像和成像分析。CellCut激光显微切割镜（Molecular Machines and Industries）已被纳入特定细胞或细胞群的靶向和捕获。另外，获得Meso Scale Discovery的SECTOR Imager 2400用于以单重或多重方式表现多种血清生物标志物，包括但不限于肌钙蛋白T和I、脂肪酸结合蛋白3、炎性细胞因子（例如，白细胞介素，TNF α，INF β），生长因子（例如，VEGF）、激素（例如，胰岛素、瘦素、雌二醇、孕酮、睾酮）。