Analytical Imaging Core

分析成像核心

• 批准号: 8377569
• 负责人: Hacene Boukari
• 金额: $ 0.84万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8377569
• 关键词: Address; Biological Process; Chemicals; Clinical Research; Commit; Complex; Core Facility; Data Analyses; Data Collection; Development; Diabetes Mellitus; Educational process of instructing; Electron Microscopy; Equipment and supply inventories; Evaluation; Experimental Designs; Freezing; Goals; Image; Imagery; Imaging Techniques; Imaging technology; Individual; Label; Laboratories; Laboratory Research; Metals; Methodology; Microscopy; Mission; Molecular; Molecular Target; Negative Staining; Optics; Organelles; Patients; Plant Resins; Postdoctoral Fellow; Preparation; Principal Investigator; Proteins; Research; Research Personnel; Research Training; Resolution; Resources; Sampling; Scientist; Services; Shadowing (Histology); Students; Techniques; Training; Work; base; cold temperature; cost effective; experience; graduate student; improved; instrumentation; light microscopy; millisecond; new technology; nucleic acid structure; pressure; research study; sample fixation

The mission of the Analytical Imaging Core Is to assist DRTC investigators in routine and complex imaging technologies for their particular research needs. This is accomplished at several levels. First, the facility has traditionally emphasized teaching new users, on an individual basis, the imaging techniques they require for successful execution of their experiments. Appropriate staff effort is expended to address the needs of these laboratories by assisting them in experimental design, data collection, quantitative analysis and presentation. Secondly, the facility offers customized full service sample preparation for electron microscopy ranging from chemical fixation, embedding in resin and ultrathin sectioning, negative staining, immunogold labeling, critical point drying and metal shadowing. In addition, the facility offers a full range of low temperature techniques for electron microscopy including quick freezing in the millisecond range by plunge, metal mirror or high pressure freezing. Thirdly, the Analytical Imaging Core develops novel technologies and state-of-the-art methodologies to continually address the ongoing needs of the Einstein DRTC research laboratories. To accomplish these goals, the Analytical Imaging Core will: 1) advise investigators, trainees and staff on the best techniques and instrumentation to address their specific research problem; 2) make available to investigators specialized light microscopy instrumentation for the analyses of cellular and molecular biological processes; 3) provide dedicated electron microscopy expertise, service and training for the high resolution visualization of cellular, organelle, protein and nucleic acid structures; 4) provide laboratory training of students, postdoctoral fellows, investigators and technical staff in performing techniques necessary to perform microscopy based analyses; 5) assist in the appropriate data analysis for the evaluation of molecular targets and biological processes; and 6) continue the development of cutting-edge imaging methodologies and instrumentation. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.

分析成像核心的使命是协助DRTC研究人员使用常规和复杂的成像技术，以满足其特定的研究需求。这是在几个层次上完成的。首先，该设施传统上强调在个人基础上教授新用户成功执行实验所需的成像技术。适当的工作人员的努力，以满足这些需求， 实验室，协助他们在实验设计，数据收集，定量分析和演示。其次，该设施提供电子显微镜样品制备的定制化全方位服务，包括化学固定、树脂包埋和切片、负染色、免疫金标记、临界点干燥和金属阴影。此外，该设施还提供全方位的低温技术 用于电子显微镜，包括通过插入、金属镜或高压冷冻在毫秒范围内快速冷冻。第三，分析成像核心开发新技术和最先进的方法，以不断满足爱因斯坦DRTC研究实验室的持续需求。为了实现这些目标，分析成像中心将：1）就最佳技术和仪器向研究人员、受训人员和工作人员提供建议，以解决他们的具体研究问题; 2）向 研究人员专门的光学显微镜仪器的细胞和分子生物学过程的分析; 3）提供专门的电子显微镜的专业知识，服务和培训的细胞，细胞器，蛋白质和核酸结构的高分辨率可视化; 4）提供实验室培训的学生，博士后研究员，研究人员和技术人员在执行必要的技术，以执行显微镜为基础的分析; 5）协助进行适当的数据分析， 评估分子靶点和生物过程; 6）继续开发尖端成像方法和仪器。所有这些服务都提供给新的糖尿病研究人员，以及从事糖尿病相关项目的研究人员，这些项目可以通过使用该核心的专业知识和设施来丰富和扩展。