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Analytical Imaging Core

分析成像核心

基本信息

批准号：
7925429
负责人：
Hacene Boukari
金额：
$ 1.66万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-01 至 2015-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7925429
关键词：
Address Arts Biological Biological Process Cells Chemicals Clinical Research Commit Complex Computer Systems Development Core Facility Cryoelectron Microscopy Cryoultramicrotomy Data Analyses Data Collection Development Diabetes Mellitus Educational process of instructing Electron Microscopy Equipment and supply inventories Evaluation Experimental Designs Fluorescence Microscopy Freeze Fracturing Freezing Goals Image Imagery Imaging Techniques Imaging technology Individual Label Laboratories Laboratory Research Mediating Metabolic Metals Methodology Microscopy Mission Molecular Molecular Target Molecular and Cellular Biology Negative Staining Obesity Optics Organelles Patients Plant Resins Postdoctoral Fellow Preparation Principal Investigator Proteins Protocols documentation Research Research Personnel Resolution Resource Sharing Resources Sampling Scientist Services Shadowing (Histology)Signal Transduction Pathway Students Techniques Time Training Work base cold temperature cost effective detection of nutrient electron tomography experience graduate student improved in vivo instrumentation light microscopy millisecond new technology nucleic acid structure pressure research study response sample fixation structural biology tool trafficking

项目摘要

The mission of the Analytical Imaging Core Is to assist DRTC investigators in routine and complex imaging technologies for their particular research needs. This is accomplished at several levels. First, the facility has traditionally emphasized teaching new users, on an individual basis, the imaging techniques they require for successful execution of their experiments. Appropriate staff effort is expended to address the needs of these laboratories by assisting them in experimental design, data collection, quantitative analysis and presentation. Secondly, the facility offers customized full service sample preparation for electron microscopy ranging from chemical fixation, embedding in resin and ultrathin sectioning, negative staining, immunogold labeling, critical point drying and metal shadowing. In addition, the facility offers a full range of low temperature techniques for electron microscopy including quick freezing in the millisecond range by plunge, metal mirror or high pressure freezing. Thirdly, the Analytical Imaging Core develops novel technologies and state-of-the-art methodologies to continually address the ongoing needs of the Einstein DRTC research laboratories. To accomplish these goals, the Analytical Imaging Core will: 1) advise investigators, trainees and staff on the best techniques and instrumentation to address their specific research problem; 2) make available to investigators specialized light microscopy instrumentation for the analyses of cellular and molecular biological processes; 3) provide dedicated electron microscopy expertise, service and training for the high resolution visualization of cellular, organelle, protein and nucleic acid structures; 4) provide laboratory training of students, postdoctoral fellows, investigators and technical staff in performing techniques necessary to perform microscopy based analyses; 5) assist in the appropriate data analysis for the evaluation of molecular targets and biological processes; and 6) continue the development of cutting-edge imaging methodologies and instrumentation. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.

分析成像核心的任务是协助DRTC调查人员采用常规和复杂的成像技术，以满足其特定的研究需求。这是在几个级别上完成的。首先，该设施传统上强调向新用户传授他们成功进行实验所需的成像技术。花费适当的工作人员努力满足这些需求通过协助实验室进行实验设计、数据收集、定量分析和陈述。其次，该设施为电子显微镜提供定制的全方位样品制备，从化学固定、树脂包埋和超薄切片、阴性染色、免疫金标记、临界点干燥和金属阴影。此外，该设施还提供全方位的低温技术。用于电子显微镜，包括通过插入、金属镜面或高压冷冻在毫秒范围内快速冻结。第三，分析成像核心开发新技术和最先进的方法，以不断满足爱因斯坦DRTC研究实验室的持续需求。为了实现这些目标，分析成像核心将：1)就解决他们的具体研究问题的最佳技术和仪器向调查人员、受训人员和工作人员提供建议；2)提供研究人员使用专门的光学显微镜仪器进行细胞和分子生物学过程的分析；3)为细胞、细胞器、蛋白质和核酸结构的高分辨率可视化提供专门的电子显微镜专业知识、服务和培训；4)为学生、博士后研究员、研究人员和技术人员提供实验室培训，以执行基于显微镜的分析所需的技术；5)协助进行适当的数据分析评估分子靶标和生物过程；以及6)继续开发尖端成像方法和仪器。所有这些服务都提供给糖尿病研究的新手，以及从事糖尿病相关项目的研究人员，这些项目可以通过使用这一核心的专门知识和设施得到丰富和扩展。